(Circular) (Six-base) MAPSORT of: pFN21AE4534.seq Check: 8025 from: 1 to: 6502 pFN21AE4534 6502 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2724 2952 3845 1937 Size: 787 228 893 4594 Fragments arranged by size: 4594 893 787 228 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3713 4565 5949 6217 1632 Size: 2081 852 1384 268 1917 Fragments arranged by size: 2081 1917 1384 852 268 AfeI AGC'GCT Cuts at: 2566 2566 Size: 6502 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 2139 3666 5401 6378 1203 Size: 936 1527 1735 977 1327 Fragments arranged by size: 1735 1527 1327 977 936 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 5231 5231 Size: 6502 AleI CACnn'nnGTG Cuts at: 1166 2482 1166 Size: 1316 5186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3398 1184 Size: 2214 4288 AlwNI CAG_nnn'CTG Cuts at: 3480 4245 5817 3480 Size: 765 1572 4165 Fragments arranged by size: 4165 1572 765 ApaLI G'TGCA_C Cuts at: 2989 4559 5715 2989 Size: 1570 1156 3776 Fragments arranged by size: 3776 1570 1156 ApoI r'AATT_y Cuts at: 1493 3811 4037 4189 6298 1493 Size: 2318 226 152 2109 1697 Fragments arranged by size: 2318 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 2789 2921 3086 3650 3827 1870 Size: 73 846 132 165 564 177 4545 Fragments arranged by size: 4545 846 564 177 165 132 73 BamHI G'GATC_C Cuts at: 2670 3470 3832 4199 2670 Size: 800 362 367 4973 Fragments arranged by size: 4973 800 367 362 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3823 5278 618 Size: 325 217 627 2036 1455 1842 Fragments arranged by size: 2036 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3270 3689 3821 1391 Size: 1879 419 132 4072 Fragments arranged by size: 4072 1879 419 132 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3334 3624 3735 928 Size: 971 1435 290 111 3695 Fragments arranged by size: 3695 1435 971 290 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4693 4727 1903 Size: 34 2756 34 3678 Fragments arranged by size: 3678 2756 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 2757 1548 Size: 1209 5293 BfrBI ATG'CAT Cuts at: 2596 2596 Size: 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 5113 136 Size: 107 122 71 631 605 397 3044 1525 Fragments arranged by size: 3044 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 2040 2877 6497 2040 Size: 837 3620 2045 Fragments arranged by size: 3620 2045 837 BlpI GC'TnA_GC Cuts at: 3919 3919 Size: 6502 Bme1580I G_kGCm'C Cuts at: 1790 2993 4563 5719 1790 Size: 1203 1570 1156 2573 Fragments arranged by size: 2573 1570 1203 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4233 5191 442 Size: 416 53 83 399 24 2816 958 1753 Fragments arranged by size: 2816 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2227 2324 5162 1239 Size: 988 97 2838 2579 Fragments arranged by size: 2838 2579 988 97 Bpu10I CC'TnA_GC Cuts at: 3756 3756 Size: 6502 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4627 5492 5790 4627 Size: 865 298 5339 Fragments arranged by size: 5339 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3314 5165 882 Size: 617 1815 1851 2219 Fragments arranged by size: 2219 1851 1815 617 BsaAI yAC'GTr Cuts at: 493 1204 2142 4293 493 Size: 711 938 2151 2702 Fragments arranged by size: 2702 2151 938 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4309 4691 5326 275 Size: 53 83 186 936 2776 382 635 1451 Fragments arranged by size: 2776 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4935 5333 5607 5754 6165 4935 Size: 398 274 147 411 5272 Fragments arranged by size: 5272 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2975 1178 Size: 30 165 30 1542 30 4705 Fragments arranged by size: 4705 1542 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3239 1186 Size: 2053 4449 BseYI C'CCAG_C Cuts at: 2896 3123 3178 3722 5705 6201 2896 Size: 227 55 544 1983 496 3197 Fragments arranged by size: 3197 1983 544 496 227 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 3184 3575 1163 Size: 573 390 1058 391 4090 Fragments arranged by size: 4090 1058 573 391 390 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2134 3952 4713 5741 1540 Size: 275 186 133 1818 761 1028 2301 Fragments arranged by size: 2301 1818 1028 761 275 186 133 BsiHKAI G_wGCw'C Cuts at: 2312 2993 3270 4563 4648 5719 2312 Size: 681 277 1293 85 1071 3095 Fragments arranged by size: 3095 1293 1071 681 277 85 BsmI GAATG_Cn' Cuts at: 4013 4106 4013 Size: 93 6409 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2993 3270 3689 3821 4563 4648 Size: 399 522 681 277 419 132 742 85 Cuts at: 4648 5719 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 681 522 419 399 277 132 85 BspEI T'CCGG_A Cuts at: 6165 6165 Size: 6502 BspHI T'CATG_A Cuts at: 4391 6121 4391 Size: 1730 4772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3857 844 Size: 3013 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4389 1394 Size: 429 2566 3507 Fragments arranged by size: 3507 2566 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3060 3273 4997 5171 65 Size: 1183 1812 213 1724 174 1396 Fragments arranged by size: 1812 1724 1396 1183 213 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5146 5333 1700 Size: 110 3336 187 2869 Fragments arranged by size: 3336 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2457 96 Size: 2361 4141 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 3392 4562 5574 3392 Size: 1170 1012 4320 Fragments arranged by size: 4320 1170 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6502 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 3118 1184 Size: 1934 4568 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2194 2569 2656 2092 Size: 102 375 87 5938 Fragments arranged by size: 5938 375 102 87 BstYI r'GATC_y Cuts at: 1972 2040 2631 2670 2877 3470 3832 4199 4584 Size: 68 591 39 207 593 362 367 385 Cuts at: 4584 4601 6053 6139 6151 6497 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 593 591 385 367 362 346 207 86 68 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3079 3079 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 2004 2483 2773 3573 3636 513 Size: 552 939 479 290 800 63 3379 Fragments arranged by size: 3379 939 800 552 479 290 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2647 487 Size: 683 56 1421 4342 Fragments arranged by size: 4342 1421 683 56 BtsI GCAGTG_nn' Cuts at: 2477 4018 4812 4832 2477 Size: 1541 794 20 4147 Fragments arranged by size: 4147 1541 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6502 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3507 3542 529 Size: 35 2943 35 3489 Fragments arranged by size: 3489 2943 35 35 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2070 2639 Size: 54 1425 50 275 18 198 42 569 Cuts at: 2639 2646 2658 3949 4838 6467 8 Size: 7 12 1291 889 1629 43 Fragments arranged by size: 1629 1425 1291 889 569 275 198 54 50 43 42 18 12 7 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 2816 3047 3161 3275 4432 1353 Size: 947 516 231 114 114 1157 3423 Fragments arranged by size: 3423 1157 947 516 231 114 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3268 3268 Size: 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2227 2324 3713 4565 5162 5949 6217 Size: 393 595 97 1389 852 597 787 268 Cuts at: 6217 1239 Size: 1524 Fragments arranged by size: 1524 1389 852 787 597 595 393 268 97 EcoO109I rG'GnC_Cy Cuts at: 2062 2287 2907 3083 3477 2062 Size: 225 620 176 394 5087 Fragments arranged by size: 5087 620 394 225 176 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 6502 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2568 5649 1678 Size: 890 3081 2531 Fragments arranged by size: 3081 2531 890 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3000 3032 3347 3379 5143 Size: 32 135 32 1428 32 315 32 1764 Cuts at: 5143 5175 5217 5249 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1764 1428 315 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2545 3436 3846 4092 669 Size: 988 281 607 891 410 246 3079 Fragments arranged by size: 3079 988 891 607 410 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 6378 6378 Size: 6502 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 3448 5616 5800 1305 Size: 1248 895 2168 184 2007 Fragments arranged by size: 2168 2007 1248 895 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 2641 2648 2660 10 Size: 54 1425 343 240 569 7 12 3852 Fragments arranged by size: 3852 1425 569 343 240 54 12 7 MslI CAynn'nnrTG Cuts at: 518 1166 2482 2946 2996 4460 4819 4978 518 Size: 648 1316 464 50 1464 359 159 2042 Fragments arranged by size: 2042 1464 1316 648 464 359 159 50 MspA1I CmG'CkG Cuts at: 1606 2520 2637 3127 3918 4595 5743 5988 1606 Size: 914 117 490 791 677 1148 245 2120 Fragments arranged by size: 2120 1148 914 791 677 490 245 117 NcoI C'CATG_G Cuts at: 513 1065 2004 3573 3636 513 Size: 552 939 1569 63 3379 Fragments arranged by size: 3379 1569 939 552 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NsiI A_TGCA'T Cuts at: 2598 2598 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 2596 3001 3670 3860 5405 1132 Size: 97 1367 405 669 190 1545 2229 Fragments arranged by size: 2229 1545 1367 669 405 190 97 PasI CC'CwG_GG Cuts at: 1348 2314 1348 Size: 966 5536 PciI A'CATG_T Cuts at: 3666 5401 3666 Size: 1735 4767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2667 3463 3610 3712 3749 1108 Size: 1559 796 147 102 37 3861 Fragments arranged by size: 3861 1559 796 147 102 37 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PmlI CAC'GTG Cuts at: 2142 2142 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2287 2907 2287 Size: 620 5882 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2405 2437 2405 Size: 32 6470 PstI C_TGCA'G Cuts at: 830 3854 830 Size: 3024 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 3127 3127 Size: 6502 SacI G_AGCT'C Cuts at: 3270 3270 Size: 6502 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SanDI GG'GwC_CC Cuts at: 2287 2907 2287 Size: 620 5882 SapI GCTCTTCn'nnn_ Cuts at: 3275 3275 Size: 6502 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 4750 6462 4750 Size: 1712 4790 SfcI C'TryA_G Cuts at: 826 1045 2533 2953 3850 4985 5666 5857 826 Size: 219 1488 420 897 1135 681 191 1471 Fragments arranged by size: 1488 1471 1135 897 681 420 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2069 2069 Size: 6502 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 2791 3088 3652 3829 1872 Size: 919 297 564 177 4545 Fragments arranged by size: 4545 919 564 297 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 4606 5507 5769 820 Size: 197 926 2663 901 262 1553 Fragments arranged by size: 2663 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 3794 1517 Size: 2277 4225 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 2796 3573 3636 3941 513 Size: 552 939 87 705 777 63 305 3074 Fragments arranged by size: 3074 939 777 705 552 305 87 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2839 3296 4529 4546 4699 4884 5329 Size: 1255 1259 457 1233 17 153 185 445 Cuts at: 5329 325 Size: 1498 Fragments arranged by size: 1498 1259 1255 1233 457 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 2457 4748 Size: 274 80 33 51 1443 345 135 2291 Cuts at: 4748 6460 96 Size: 1712 138 Fragments arranged by size: 2291 1712 1443 345 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2221 2253 2814 2846 6462 6494 2221 Size: 32 561 32 3616 32 2229 Fragments arranged by size: 3616 2229 561 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 3838 3838 Size: 6502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2092 2536 2569 3067 3464 2092 Size: 444 33 498 397 5130 Fragments arranged by size: 5130 498 444 397 33 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6502 XmaI C'CCGG_G Cuts at: 1870 2789 3086 3650 3827 1870 Size: 919 297 564 177 4545 Fragments arranged by size: 4545 919 564 297 177 XmnI GAAnn'nnTTC Cuts at: 4631 4631 Size: 6502 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI RsrII SacII SexAI SfoI SrfI SwaI