################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BcnI BglI BlnI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmBI BsmI BspHI BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FbaI FseI FspI HaeII Hin1I KasI KpnI KspI MamI MluI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 887 GTCTAC GT^[AC][GT]AC AccIII 3008 TCCGGA T^CCGGA AflII 2188 CTTAAG C^TTAAG AflIII 329 ACATGT A^C[GA][CT]GT Alw44I 2358 GTGCAC G^TGCAC ApaLI 2358 GTGCAC G^TGCAC Asp700I 1687 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 2782 CTCGAG C^[CT]CG[GA]G BanII 2056 GGGCTC G[GA]GC[CT]^C BbsI 2749 GAAGAC GAAGAC(2/6) BcgI 2340 GCATCATAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2188 CTTAAG C^TTAAG BpuAI 2749 GAAGAC GAAGAC(2/6) BsaWI 3008 TCCGGA [AT]^CCGG[AT] BseAI 3008 TCCGGA T^CCGGA BsgI 150 CTGCAC GTGCAG(16/14) BsoBI 2782 CTCGAG C^[CT]CG[GA]G Bsp1407I 2489 TGTACA T^GTACA BspDI 280 ATCGAT AT^CGAT BspEI 3008 TCCGGA T^CCGGA BspLU11I 329 ACATGT A^CATGT BsrFI 1655 ACCGGC [GA]^CCGG[CT] BsrGI 2489 TGTACA T^GTACA BstEII 2798 GGTTACC G^GT[ACGT]ACC BstPI 2798 GGTTACC G^GT[ACGT]ACC BstXI 3067 CCATACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 3057 GCAAGC GC[ACGT]^[ACGT]GC Cfr10I 1655 ACCGGC [GA]^CCGG[CT] ClaI 280 ATCGAT AT^CGAT DsaI 1844 CCATGG C^C[GA][CT]GG EcoO65I 2798 GGTTACC G^GT[ACGT]ACC EcoRV 2249 GATATC GAT^ATC HincII 2392 GTTAAC GT[CT]^[GA]AC HindII 2392 GTTAAC GT[CT]^[GA]AC HpaI 2392 GTTAAC GTT^AAC MaeII 885 ACGT A^CGT MfeI 1030 CAATTG C^AATTG MroI 3008 TCCGGA T^CCGGA MspA1I 2705 CAGCTG C[AC]G^C[GT]G MunI 1030 CAATTG C^AATTG NcoI 1844 CCATGG C^CATGG NdeI 1759 CATATG CA^TATG NlaIV 2445 GGGGCC GG[ACGT]^[ACGT]CC PaeR7I 2782 CTCGAG C^TCGAG PpuMI 1373 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 2133 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 2705 CAGCTG CAG^CTG SapI 1687 GAAGAGC GCTCTTC(1/4) SspBI 2489 TGTACA T^GTACA XhoI 2782 CTCGAG C^TCGAG XmnI 1687 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 301 GTAC GT^AC 2490 GTAC AhdI 1265 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2571 GACAGCAAGTC AspEI 1265 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2571 GACAGCAAGTC BbvCI 1239 CCTCAGC CCTCAGC(-5/-2) 2115 CCTCAGC BglII 1278 AGATCT A^GATCT 2505 AGATCT BlpI 2728 GCTGAGC GC^T[ACGT]AGC 2844 GCTTAGC Bpu1102I 2728 GCTGAGC GC^T[ACGT]AGC 2844 GCTTAGC CelII 2728 GCTGAGC GC^T[ACGT]AGC 2844 GCTTAGC CfoI 811 GCGC GCG^C 862 GCGC DraII 431 AGGGCCT [GA]G^G[ACGT]CC[CT] 1373 AGGTCCT Eam1105I 1265 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2571 GACAGCAAGTC EcoO109I 431 AGGGCCT [GA]G^G[ACGT]CC[CT] 1373 AGGTCCT EcoRI 1609 GAATTC G^AATTC 1873 GAATTC FauI 438 CCCGC CCCGC(4/6) 3054 CCCGC HapII 1656 CCGG C^CGG 3009 CCGG HgaI 247 GCGTC GACGC(5/10) 2646 GCGTC HhaI 811 GCGC GCG^C 862 GCGC HinP1I 811 GCGC G^CGC 862 GCGC HindIII 363 AAGCTT A^AGCTT 1158 AAGCTT HpaII 1656 CCGG C^CGG 3009 CCGG MspI 1656 CCGG C^CGG 3009 CCGG MwoI 853 GCTTTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1721 GCAGTAATAGC PflMI 948 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1371 CCAGGTCCTGG PstI 346 CTGCAG CTGCA^G 1792 CTGCAG RsaI 301 GTAC GT^AC 2490 GTAC Van91I 948 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1371 CCAGGTCCTGG XcmI 1590 CCAAACTCTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1835 CCAGCAGGACCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AspHI AvaII BalI BbvI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI NdeII NlaIII NspI PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII