(Circular) (Six-base) MAPSORT of: pFN21AE5001.seq Check: 2441 from: 1 to: 7852 pFN21AE5001 7852 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5662 278 Size: 53 83 186 936 4126 2468 Fragments arranged by size: 4126 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2893 5195 1937 Size: 956 2302 4594 Fragments arranged by size: 4594 2302 956 Acc65I G'GTAC_C Cuts at: 5173 5173 Size: 7852 AclI AA'CG_TT Cuts at: 1563 5979 6352 1563 Size: 4416 373 3063 Fragments arranged by size: 4416 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 2894 3047 3294 4349 5915 7299 7567 Size: 416 846 153 247 1055 1566 1384 268 Cuts at: 7567 1632 Size: 1917 Fragments arranged by size: 1917 1566 1384 1055 846 416 268 247 153 AflII C'TTAA_G Cuts at: 820 1017 4193 820 Size: 197 3176 4479 Fragments arranged by size: 4479 3176 197 AflIII A'CryG_T Cuts at: 1203 2334 6751 7728 1203 Size: 1131 4417 977 1327 Fragments arranged by size: 4417 1327 1131 977 AgeI A'CCGG_T Cuts at: 6683 6683 Size: 7852 AhdI GACnn_n'nnGTC Cuts at: 3275 4581 6581 3275 Size: 1306 2000 4546 Fragments arranged by size: 4546 2000 1306 AleI CACnn'nnGTG Cuts at: 1166 4436 1166 Size: 3270 4582 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3442 1184 Size: 2258 5594 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3363 3395 3363 Size: 32 7820 AlwNI CAG_nnn'CTG Cuts at: 2959 3382 3805 5595 7167 2959 Size: 423 423 1790 1572 3644 Fragments arranged by size: 3644 1790 1572 423 423 ApaLI G'TGCA_C Cuts at: 4363 5909 7065 4363 Size: 1546 1156 5150 Fragments arranged by size: 5150 1546 1156 ApoI r'AATT_y Cuts at: 1493 2037 2178 2760 3005 3123 3614 3878 3982 Size: 544 141 582 245 118 491 264 104 Cuts at: 3982 4900 5161 5387 5539 7648 1493 Size: 918 261 226 152 2109 1697 Fragments arranged by size: 2109 1697 918 582 544 491 264 261 245 226 152 141 118 104 AseI AT'TA_AT Cuts at: 160 1032 6406 160 Size: 872 5374 1606 Fragments arranged by size: 5374 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7852 AvaI C'yCGr_G Cuts at: 1870 1943 4787 5177 1870 Size: 73 2844 390 4545 Fragments arranged by size: 4545 2844 390 73 BamHI G'GATC_C Cuts at: 5182 5549 5182 Size: 367 7485 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5173 6628 618 Size: 325 217 627 3386 1455 1842 Fragments arranged by size: 3386 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4065 5171 1391 Size: 2674 1106 4072 Fragments arranged by size: 4072 2674 1106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4761 928 Size: 971 2862 4019 Fragments arranged by size: 4019 2862 971 BbvCI CC'TCA_GC Cuts at: 3245 4121 3245 Size: 876 6976 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4334 4368 6043 6077 1903 Size: 34 2397 34 1675 34 3678 Fragments arranged by size: 3678 2397 1675 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5744 6954 5744 Size: 1210 6642 BclI T'GATC_A Cuts at: 1548 1548 Size: 7852 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6463 136 Size: 107 122 71 631 605 4791 1525 Fragments arranged by size: 4791 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3283 4510 7847 3283 Size: 1227 3337 3288 Fragments arranged by size: 3337 3288 1227 BlpI GC'TnA_GC Cuts at: 4734 4850 5269 4734 Size: 116 419 7317 Fragments arranged by size: 7317 419 116 Bme1580I G_kGCm'C Cuts at: 1790 2922 4367 5913 7069 1790 Size: 1132 1445 1546 1156 2573 Fragments arranged by size: 2573 1546 1445 1156 1132 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7852 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2327 5583 6541 Size: 416 53 83 399 24 910 3256 958 Cuts at: 6541 442 Size: 1753 Fragments arranged by size: 3256 1753 958 910 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7852 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3879 3879 Size: 7852 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 2936 4026 4073 4991 6512 1239 Size: 966 731 1090 47 918 1521 2579 Fragments arranged by size: 2579 1521 1090 966 918 731 47 Bpu10I CC'TnA_GC Cuts at: 3245 4121 3245 Size: 876 6976 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2489 4163 4997 5063 5977 6842 7140 2489 Size: 1674 834 66 914 865 298 3201 Fragments arranged by size: 3201 1674 914 865 834 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6515 882 Size: 617 5016 2219 Fragments arranged by size: 5016 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5643 493 Size: 711 4439 2702 Fragments arranged by size: 4439 2702 711 BsaBI GATnn'nnATC Cuts at: 7846 7846 Size: 7852 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5659 6041 6676 275 Size: 53 83 186 936 4126 382 635 1451 Fragments arranged by size: 4126 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5013 6285 6683 6957 7104 7515 5013 Size: 1272 398 274 147 411 5350 Fragments arranged by size: 5350 1272 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3845 3875 3901 3931 1178 Size: 30 165 30 2442 30 26 30 5099 Fragments arranged by size: 5099 2442 165 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7852 BseYI C'CCAG_C Cuts at: 2248 3023 4708 7055 7551 2248 Size: 775 1685 2347 496 2549 Fragments arranged by size: 2549 2347 1685 775 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2140 1163 Size: 573 404 6875 Fragments arranged by size: 6875 573 404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5151 5302 6063 7091 1540 Size: 275 186 3150 151 761 1028 2301 Fragments arranged by size: 3150 2301 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2631 4367 4462 5913 5998 7069 2631 Size: 1736 95 1451 85 1071 3414 Fragments arranged by size: 3414 1736 1451 1071 95 85 BsmI GAATG_Cn' Cuts at: 5363 5456 5363 Size: 93 7759 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2631 2922 4065 4367 4462 5171 5913 Size: 399 841 291 1143 302 95 709 742 Cuts at: 5913 5998 7069 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1143 1071 841 742 709 399 302 291 95 85 BspEI T'CCGG_A Cuts at: 5013 7515 5013 Size: 2502 5350 BspHI T'CATG_A Cuts at: 5741 7471 5741 Size: 1730 6122 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5207 844 Size: 4363 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5739 1394 Size: 429 3916 3507 Fragments arranged by size: 3916 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6347 6521 65 Size: 1183 5099 174 1396 Fragments arranged by size: 5099 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3660 6496 6683 1700 Size: 110 1850 2836 187 2869 Fragments arranged by size: 2869 2836 1850 187 110 BsrGI T'GTAC_A Cuts at: 96 4494 96 Size: 4398 3454 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7852 BssSI C'ACGA_G Cuts at: 5912 6924 5912 Size: 1012 6840 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7852 BstBI TT'CG_AA Cuts at: 6656 6656 Size: 7852 BstEII G'GTnAC_C Cuts at: 1184 4803 1184 Size: 3619 4233 BstXI CCAn_nnnn'nTGG Cuts at: 5079 5079 Size: 7852 BstYI r'GATC_y Cuts at: 1972 2844 2962 3040 3283 3499 4261 4489 4510 Size: 872 118 78 243 216 762 228 21 Cuts at: 4510 5182 5549 5934 5951 7403 7489 7501 7847 Size: 672 367 385 17 1452 86 12 346 Cuts at: 7847 1972 Size: 1977 Fragments arranged by size: 1977 1452 872 762 672 385 367 346 243 228 216 118 86 78 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3849 513 Size: 552 939 1845 4516 Fragments arranged by size: 4516 1845 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7113 Fragments arranged by size: 7113 683 56 BtsI GCAGTG_nn' Cuts at: 3616 5090 5368 6162 6182 3616 Size: 1474 278 794 20 5286 Fragments arranged by size: 5286 1474 794 278 20 ClaI AT'CG_AT Cuts at: 1554 2286 1554 Size: 732 7120 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7817 DraI TTT'AAA Cuts at: 2391 3151 3265 5156 2391 Size: 760 114 1891 5087 Fragments arranged by size: 5087 1891 760 114 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6859 809 Size: 507 5543 1802 Fragments arranged by size: 5543 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2517 2982 4050 Size: 54 1425 50 275 18 687 465 1068 Cuts at: 4050 4428 4743 5048 5103 5299 6188 7817 8 Size: 378 315 305 55 196 889 1629 43 Fragments arranged by size: 1629 1425 1068 889 687 465 378 315 305 275 196 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5299 1537 Size: 275 3487 4090 Fragments arranged by size: 4090 3487 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2913 3687 3835 5782 1353 Size: 671 889 774 148 1947 3423 Fragments arranged by size: 3423 1947 889 774 671 148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6439 6813 6959 387 Size: 1064 138 4850 374 146 1280 Fragments arranged by size: 4850 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2205 2894 2936 3047 3294 4026 Size: 393 416 157 689 42 111 247 732 Cuts at: 4026 4073 4349 4991 5915 6512 7299 7567 1239 Size: 47 276 642 924 597 787 268 1524 Fragments arranged by size: 1524 924 787 732 689 642 597 416 393 276 268 247 157 111 47 42 EcoO109I rG'GnC_Cy Cuts at: 2437 3379 2437 Size: 942 6910 EcoRI G'AATT_C Cuts at: 3614 3878 5161 5539 3614 Size: 264 1283 378 5927 Fragments arranged by size: 5927 1283 378 264 EcoRV GAT'ATC Cuts at: 4256 4256 Size: 7852 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4318 4318 Size: 7852 FspI TGC'GCA Cuts at: 6358 6358 Size: 7852 HaeII r_GCGC'y Cuts at: 1678 6999 1678 Size: 5321 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3129 3161 3261 3293 3484 Size: 32 135 32 1557 32 100 32 191 Cuts at: 3484 3516 3845 3877 4142 4174 4253 4285 4567 Size: 32 329 32 265 32 79 32 282 Cuts at: 4567 4599 6493 6525 6567 6599 1373 Size: 32 1894 32 42 32 2626 Fragments arranged by size: 2626 1894 1557 329 282 265 191 135 100 79 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4399 5196 5442 669 Size: 988 281 2461 797 246 3079 Fragments arranged by size: 3079 2461 988 797 281 246 HindIII A'AGCT_T Cuts at: 748 2368 3163 748 Size: 1620 795 5437 Fragments arranged by size: 5437 1620 795 HpaI GTT'AAC Cuts at: 4399 5442 4399 Size: 1043 6809 KpnI G_GTAC'C Cuts at: 5177 5177 Size: 7852 MfeI C'AATT_G Cuts at: 3035 5451 3035 Size: 2416 5436 MluI A'CGCG_T Cuts at: 7728 7728 Size: 7852 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3643 3678 4058 4185 6966 7150 1305 Size: 2338 35 380 127 2781 184 2007 Fragments arranged by size: 2781 2338 2007 380 184 127 35 MscI TGG'CCA Cuts at: 10 64 1489 1832 2519 2984 4052 4430 4745 Size: 54 1425 343 687 465 1068 378 315 Cuts at: 4745 5050 5105 10 Size: 305 55 2757 Fragments arranged by size: 2757 1425 1068 687 465 378 343 315 305 55 54 MslI CAynn'nnrTG Cuts at: 518 1166 3897 4436 4568 5810 6169 6328 518 Size: 648 2731 539 132 1242 359 159 2042 Fragments arranged by size: 2731 2042 1242 648 539 359 159 132 MspA1I CmG'CkG Cuts at: 1606 4712 5268 5945 7093 7338 1606 Size: 3106 556 677 1148 245 2120 Fragments arranged by size: 3106 2120 1148 677 556 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3849 513 Size: 552 939 1845 4516 Fragments arranged by size: 4516 1845 939 552 NdeI CA'TA_TG Cuts at: 387 3765 387 Size: 3378 4474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7852 NotI GC'GGCC_GC Cuts at: 5299 5299 Size: 7852 NspI r_CATG'y Cuts at: 1132 1229 2338 2918 3253 5210 6755 1132 Size: 97 1109 580 335 1957 1545 2229 Fragments arranged by size: 2229 1957 1545 1109 580 335 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7852 PciI A'CATG_T Cuts at: 2334 6751 2334 Size: 4417 3435 PflMI CCAn_nnn'nTGG Cuts at: 1108 2959 3382 1108 Size: 1851 423 5578 Fragments arranged by size: 5578 1851 423 PfoI T'CCnGG_A Cuts at: 2182 2952 3381 2182 Size: 770 429 6653 Fragments arranged by size: 6653 770 429 PmeI GTTT'AAAC Cuts at: 5156 5156 Size: 7852 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2682 2714 5921 5953 7434 7466 2682 Size: 32 3207 32 1481 32 3068 Fragments arranged by size: 3207 3068 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3379 3379 Size: 7852 PshAI GACnn'nnGTC Cuts at: 1613 4142 1613 Size: 2529 5323 PsiI TTA'TAA Cuts at: 2796 3230 5422 2796 Size: 434 2192 5226 Fragments arranged by size: 5226 2192 434 PstI C_TGCA'G Cuts at: 830 2355 3801 5204 830 Size: 1525 1446 1403 3478 Fragments arranged by size: 3478 1525 1446 1403 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7852 PvuII CAG'CTG Cuts at: 4712 4712 Size: 7852 SalI G'TCGA_C Cuts at: 1936 5194 1936 Size: 3258 4594 SapI GCTCTTCn'nnn_ Cuts at: 3687 3687 Size: 7852 SbfI CC_TGCA'GG Cuts at: 5204 5204 Size: 7852 ScaI AGT'ACT Cuts at: 6100 7812 6100 Size: 1712 6140 SfcI C'TryA_G Cuts at: 826 1045 2351 2840 3357 3797 5125 5200 6335 Size: 219 1306 489 517 440 1328 75 1135 Cuts at: 6335 7016 7207 826 Size: 681 191 1471 Fragments arranged by size: 1471 1328 1306 1135 681 517 489 440 219 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7852 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7852 SmaI CCC'GGG Cuts at: 1872 5179 1872 Size: 3307 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2468 4178 4193 4787 4976 5042 Size: 197 926 525 1710 15 594 189 66 Cuts at: 5042 5956 6857 7119 820 Size: 914 901 262 1553 Fragments arranged by size: 1710 1553 926 914 901 594 525 262 197 189 66 15 SnaBI TAC'GTA Cuts at: 493 5643 493 Size: 5150 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7852 SphI G_CATG'C Cuts at: 1132 5210 1132 Size: 4078 3774 SspI AAT'ATT Cuts at: 5 52 5776 5 Size: 47 5724 2081 Fragments arranged by size: 5724 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7852 StyI C'CwwG_G Cuts at: 513 1065 2004 2077 3495 3849 5291 513 Size: 552 939 73 1418 354 1442 3074 Fragments arranged by size: 3074 1442 1418 939 552 354 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4733 5085 5879 5896 6049 6234 6679 Size: 1255 3153 352 794 17 153 185 445 Cuts at: 6679 325 Size: 1498 Fragments arranged by size: 3153 1498 1255 794 445 352 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4494 6098 7810 Size: 274 80 33 51 1443 2517 1604 1712 Cuts at: 7810 96 Size: 138 Fragments arranged by size: 2517 1712 1604 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3901 3933 7812 7844 3901 Size: 32 3879 32 3909 Fragments arranged by size: 3909 3879 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7852 XbaI T'CTAG_A Cuts at: 5188 5188 Size: 7852 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3602 3847 3602 Size: 245 7607 XhoI C'TCGA_G Cuts at: 1943 4787 1943 Size: 2844 5008 XmaI C'CCGG_G Cuts at: 1870 5177 1870 Size: 3307 4545 XmnI GAAnn'nnTTC Cuts at: 3696 5981 3696 Size: 2285 5567 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5660 276 Size: 53 83 186 936 4126 2468 Fragments arranged by size: 4126 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI