(Linear) (Six-base) MAPSORT of: KIEE5001.seq Check: 6286 from: 1 to: 3147 KIEE5001.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AccI GT'mk_AC Cuts at: 0 888 3147 Size: 888 2259 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 889 1042 1289 2344 3147 Size: 43 846 153 247 1055 803 Fragments arranged by size: 1055 846 803 247 153 43 AflII C'TTAA_G Cuts at: 0 2188 3147 Size: 2188 959 AflIII A'CryG_T Cuts at: 0 329 3147 Size: 329 2818 AhdI GACnn_n'nnGTC Cuts at: 0 1270 2576 3147 Size: 1270 1306 571 Fragments arranged by size: 1306 1270 571 AleI CACnn'nnGTG Cuts at: 0 2431 3147 Size: 2431 716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1437 3147 Size: 1437 1710 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1358 1390 3147 Size: 1358 32 1757 Fragments arranged by size: 1757 1358 32 AlwNI CAG_nnn'CTG Cuts at: 0 954 1377 1800 3147 Size: 954 423 423 1347 Fragments arranged by size: 1347 954 423 423 ApaLI G'TGCA_C Cuts at: 0 2358 3147 Size: 2358 789 ApoI r'AATT_y Cuts at: 0 32 173 755 1000 1118 1609 1873 1977 Size: 32 141 582 245 118 491 264 104 Cuts at: 1977 2895 3147 Size: 918 252 Fragments arranged by size: 918 582 491 264 252 245 141 118 104 32 AvaI C'yCGr_G Cuts at: 0 2782 3147 Size: 2782 365 BanII G_rGCy'C Cuts at: 0 2060 3147 Size: 2060 1087 BbsI GAAGACnn'nnnn_ Cuts at: 0 2756 3147 Size: 2756 391 BbvCI CC'TCA_GC Cuts at: 0 1240 2116 3147 Size: 1240 876 1031 Fragments arranged by size: 1240 1031 876 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2329 2363 3147 Size: 2329 34 784 Fragments arranged by size: 2329 784 34 BglII A'GATC_T Cuts at: 0 1278 2505 3147 Size: 1278 1227 642 Fragments arranged by size: 1278 1227 642 BlpI GC'TnA_GC Cuts at: 0 2729 2845 3147 Size: 2729 116 302 Fragments arranged by size: 2729 302 116 Bme1580I G_kGCm'C Cuts at: 0 917 2362 3147 Size: 917 1445 785 Fragments arranged by size: 1445 917 785 BmrI ACTGGGnnnn_n' Cuts at: 0 322 3147 Size: 322 2825 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1874 3147 Size: 1874 1273 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 931 2021 2068 2986 3147 Size: 200 731 1090 47 918 161 Fragments arranged by size: 1090 918 731 200 161 47 Bpu10I CC'TnA_GC Cuts at: 0 1240 2116 3147 Size: 1240 876 1031 Fragments arranged by size: 1240 1031 876 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 484 2158 2992 3058 3147 Size: 484 1674 834 66 89 Fragments arranged by size: 1674 834 484 89 66 BsaWI w'CCGG_w Cuts at: 0 3008 3147 Size: 3008 139 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1840 1870 1896 1926 3147 Size: 1840 30 26 30 1221 Fragments arranged by size: 1840 1221 30 30 26 BseYI C'CCAG_C Cuts at: 0 243 1018 2703 3147 Size: 243 775 1685 444 Fragments arranged by size: 1685 775 444 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 135 3147 Size: 135 3012 BsiHKAI G_wGCw'C Cuts at: 0 626 2362 2457 3147 Size: 626 1736 95 690 Fragments arranged by size: 1736 690 626 95 Bsp1286I G_dGCh'C Cuts at: 0 626 917 2060 2362 2457 3147 Size: 626 291 1143 302 95 690 Fragments arranged by size: 1143 690 626 302 291 95 BspEI T'CCGG_A Cuts at: 0 3008 3147 Size: 3008 139 BsrFI r'CCGG_y Cuts at: 0 1655 3147 Size: 1655 1492 BsrGI T'GTAC_A Cuts at: 0 2489 3147 Size: 2489 658 BstEII G'GTnAC_C Cuts at: 0 2798 3147 Size: 2798 349 BstXI CCAn_nnnn'nTGG Cuts at: 0 3074 3147 Size: 3074 73 BstYI r'GATC_y Cuts at: 0 839 957 1035 1278 1494 2256 2484 2505 Size: 839 118 78 243 216 762 228 21 Cuts at: 2505 3147 Size: 642 Fragments arranged by size: 839 762 642 243 228 216 118 78 21 BtgI C'CryG_G Cuts at: 0 1844 3147 Size: 1844 1303 BtsI GCAGTG_nn' Cuts at: 0 1611 3085 3147 Size: 1611 1474 62 Fragments arranged by size: 1611 1474 62 ClaI AT'CG_AT Cuts at: 0 281 3147 Size: 281 2866 DraI TTT'AAA Cuts at: 0 386 1146 1260 3147 Size: 386 760 114 1887 Fragments arranged by size: 1887 760 386 114 EaeI y'GGCC_r Cuts at: 0 512 977 2045 2423 2738 3043 3098 3147 Size: 512 465 1068 378 315 305 55 49 Fragments arranged by size: 1068 512 465 378 315 305 55 49 EarI CTCTTCn'nnn_ Cuts at: 0 19 908 1682 1830 3147 Size: 19 889 774 148 1317 Fragments arranged by size: 1317 889 774 148 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 43 200 889 931 1042 1289 2021 2068 Size: 43 157 689 42 111 247 732 47 Cuts at: 2068 2344 2986 3147 Size: 276 642 161 Fragments arranged by size: 732 689 642 276 247 161 157 111 47 43 42 EcoO109I rG'GnC_Cy Cuts at: 0 432 1374 3147 Size: 432 942 1773 Fragments arranged by size: 1773 942 432 EcoRI G'AATT_C Cuts at: 0 1609 1873 3147 Size: 1609 264 1274 Fragments arranged by size: 1609 1274 264 EcoRV GAT'ATC Cuts at: 0 2251 3147 Size: 2251 896 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2313 3147 Size: 2313 834 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1124 1156 1256 1288 1479 1511 1840 1872 Size: 1124 32 100 32 191 32 329 32 Cuts at: 1872 2137 2169 2248 2280 2562 2594 3147 Size: 265 32 79 32 282 32 553 Fragments arranged by size: 1124 553 329 282 265 191 100 79 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2394 3147 Size: 2394 753 HindIII A'AGCT_T Cuts at: 0 363 1158 3147 Size: 363 795 1989 Fragments arranged by size: 1989 795 363 HpaI GTT'AAC Cuts at: 0 2394 3147 Size: 2394 753 MfeI C'AATT_G Cuts at: 0 1030 3147 Size: 1030 2117 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1638 1673 2053 2180 3147 Size: 1638 35 380 127 967 Fragments arranged by size: 1638 967 380 127 35 MscI TGG'CCA Cuts at: 0 514 979 2047 2425 2740 3045 3100 3147 Size: 514 465 1068 378 315 305 55 47 Fragments arranged by size: 1068 514 465 378 315 305 55 47 MslI CAynn'nnrTG Cuts at: 0 1892 2431 2563 3147 Size: 1892 539 132 584 Fragments arranged by size: 1892 584 539 132 MspA1I CmG'CkG Cuts at: 0 2707 3147 Size: 2707 440 NcoI C'CATG_G Cuts at: 0 1844 3147 Size: 1844 1303 NdeI CA'TA_TG Cuts at: 0 1760 3147 Size: 1760 1387 NspI r_CATG'y Cuts at: 0 333 913 1248 3147 Size: 333 580 335 1899 Fragments arranged by size: 1899 580 335 333 PciI A'CATG_T Cuts at: 0 329 3147 Size: 329 2818 PflMI CCAn_nnn'nTGG Cuts at: 0 954 1377 3147 Size: 954 423 1770 Fragments arranged by size: 1770 954 423 PfoI T'CCnGG_A Cuts at: 0 177 947 1376 3147 Size: 177 770 429 1771 Fragments arranged by size: 1771 770 429 177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 677 709 3147 Size: 677 32 2438 Fragments arranged by size: 2438 677 32 PpuMI rG'GwC_Cy Cuts at: 0 1374 3147 Size: 1374 1773 PshAI GACnn'nnGTC Cuts at: 0 2137 3147 Size: 2137 1010 PsiI TTA'TAA Cuts at: 0 791 1225 3147 Size: 791 434 1922 Fragments arranged by size: 1922 791 434 PstI C_TGCA'G Cuts at: 0 350 1796 3147 Size: 350 1446 1351 Fragments arranged by size: 1446 1351 350 PvuII CAG'CTG Cuts at: 0 2707 3147 Size: 2707 440 SapI GCTCTTCn'nnn_ Cuts at: 0 1682 3147 Size: 1682 1465 SfcI C'TryA_G Cuts at: 0 346 835 1352 1792 3120 3147 Size: 346 489 517 440 1328 27 Fragments arranged by size: 1328 517 489 440 346 27 SmlI C'TyrA_G Cuts at: 0 463 2173 2188 2782 2971 3037 3147 Size: 463 1710 15 594 189 66 110 Fragments arranged by size: 1710 594 463 189 110 66 15 StyI C'CwwG_G Cuts at: 0 72 1490 1844 3147 Size: 72 1418 354 1303 Fragments arranged by size: 1418 1303 354 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2728 3080 3147 Size: 2728 352 67 Fragments arranged by size: 2728 352 67 TatI w'GTAC_w Cuts at: 0 2489 3147 Size: 2489 658 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1896 1928 3147 Size: 1896 32 1219 Fragments arranged by size: 1896 1219 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1597 1842 3147 Size: 1597 245 1305 Fragments arranged by size: 1597 1305 245 XhoI C'TCGA_G Cuts at: 0 2782 3147 Size: 2782 365 XmnI GAAnn'nnTTC Cuts at: 0 1691 3147 Size: 1691 1456 Enzymes that do cut: AccI AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrFI BsrGI BstEII BstXI BstYI BtgI BtsI ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsiI PstI PvuII SapI SfcI SmlI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI BclI BfrBI BglI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI FseI FspI FspAI HaeII KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI