Restriction map for
pFN21AE4451
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BclI BcnI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MamI
MluI MluNI MroI MscI NaeI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 1044 GGCGCC G[GA]^CG[CT]C
AflII 151 CTTAAG C^TTAAG
AflIII 363 ACGTGT A^C[GA][CT]GT
AlwNI 374 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 19 GGGCCC GGGCC^C
AseI 1371 ATTAAT AT^TAAT
AsnI 1371 ATTAAT AT^TAAT
BanI 1044 GGCGCC G^G[CT][GA]CC
BbeI 1044 GGCGCC GGCGC^C
BciVI 708 GTATCC GTATCC(6/5)
BfrI 151 CTTAAG C^TTAAG
BsaAI 362 TACGTG [CT]AC^GT[GA]
BsaHI 1044 GGCGCC G[GA]^CG[CT]C
BsgI 852 CTGCAC GTGCAG(16/14)
BsmAI 848 GTCTC GTCTC(1/5)
BsmFI 433 GGGAC GGGAC(10/14)
BspHI 1220 TCATGA T^CATGA
BsrFI 486 ACCGGC [GA]^CCGG[CT]
BstEII 987 GGTAACC G^GT[ACGT]ACC
BstPI 987 GGTAACC G^GT[ACGT]ACC
BstYI 1324 GGATCT [GA]^GATC[CT]
Cfr10I 486 ACCGGC [GA]^CCGG[CT]
DsaI 1040 CCATGG C^C[GA][CT]GG
EarI 898 GAAGAG CTCTTC(1/4)
EcoO65I 987 GGTAACC G^GT[ACGT]ACC
EcoT14I 1040 CCATGG C^C[AT][AT]GG
HaeIII 20 GGCC GG^CC
HapII 487 CCGG C^CGG
Hin1I 1044 GGCGCC G[GA]^CG[CT]C
HpaII 487 CCGG C^CGG
KasI 1044 GGCGCC G^GCGCC
Ksp632I 898 GAAGAG CTCTTC(1/4)
MflI 1324 GGATCT [GA]^GATC[CT]
MslI 199 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 487 CCGG C^CGG
NarI 1044 GGCGCC GG^CGCC
NcoI 1040 CCATGG C^CATGG
PshBI 1371 ATTAAT AT^TAAT
RcaI 1220 TCATGA T^CATGA
ScaI 861 AGTACT AGT^ACT
SfoI 1044 GGCGCC GGC^GCC
SmlI 151 CTTAAG C^T[CT][GA]AG
StyI 1040 CCATGG C^C[AT][AT]GG
TaqI 1190 TCGA T^CGA
TfiI 1192 GATTC G^A[AT]TC
TthHB8I 1190 TCGA T^CGA
XcmI 1040 CCATGGCGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1324 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 619 GTAGAC GT^[AC][GT]AC
1108 GTAGAC
AlwI 583 GGATC GGATC(4/5)
1324 GGATC
AspI 376 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
972 GACACTGTC
BanII 19 GGGCCC G[GA]GC[CT]^C
787 GGGCTC
BmyI 19 GGGCCC G[AGT]GC[ACT]^C
787 GGGCTC
BsaJI 1040 CCATGG C^C[ACGT][ACGT]GG
1271 CCCAGG
Bsp1286I 19 GGGCCC G[AGT]GC[ACT]^C
787 GGGCTC
BsrI 437 CCAGT ACTGG(1/-1)
1378 CCAGT
BssHII 26 GCGCGC G^CGCGC
95 GCGCGC
BssKI 476 CCTGG ^CC[ACGT]GG
1272 CCAGG
BstNI 476 CCTGG CC^[AT]GG
1272 CCAGG
DdeI 1124 CTCAG C^T[ACGT]AG
1139 CTGAG
DraII 18 AGGGCCC [GA]G^G[ACGT]CC[CT]
19 GGGCCCC
EcoO109I 18 AGGGCCC [GA]G^G[ACGT]CC[CT]
19 GGGCCCC
EcoRII 476 CCTGG ^CC[AT]GG
1272 CCAGG
HphI 835 GGTGA GGTGA(8/7)
473 TCACC
MfeI 244 CAATTG C^AATTG
1316 CAATTG
MspA1I 52 CAGCTG C[AC]G^C[GT]G
696 CAGCTG
MunI 244 CAATTG C^AATTG
1316 CAATTG
MvaI 476 CCTGG CC^[AT]GG
1272 CCAGG
PflFI 376 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
972 GACACTGTC
PleI 376 GACTC GAGTC(4/5)
480 GACTC
PvuII 52 CAGCTG CAG^CTG
696 CAGCTG
ScrFI 476 CCTGG CC^[ACGT]GG
1272 CCAGG
TspRI 468 CACTG CA[GC]TG[ACGT][ACGT]^
974 CACTG
Tth111I 376 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
972 GACACTGTC
XbaI 603 TCTAGA T^CTAGA
1165 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaII BbsI BbvI BfaI
BpuAI BsiYI BslI BstF5I BstUI
Cac8I CfoI Cfr13I DpnII FauI
Fnu4HI FokI HaeII HhaI HinP1I
HinfI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MvnI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I Tru9I TseI Tsp45I
Tsp509I