################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvnI MwoI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tsp45I Tth111I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Alw44I 555 GTGCAC G^TGCAC ApaLI 555 GTGCAC G^TGCAC AspHI 555 GTGCAC G[AT]GC[AT]^C AvaI 992 CTCGAG C^[CT]CG[GA]G AvaII 527 GGACC G^G[AT]CC BanII 755 GAGCCC G[GA]GC[CT]^C BbsI 291 GAAGAC GAAGAC(2/6) BclI 300 TGATCA T^GATCA BglII 401 AGATCT A^GATCT BpmI 745 CTGGAG CTGGAG(16/14) BpuAI 291 GAAGAC GAAGAC(2/6) BsaI 387 GGTCTC GGTCTC(1/5) BsgI 62 GTGCAG GTGCAG(16/14) BsiHKAI 555 GTGCAC G[AT]GC[AT]^C BsmI 84 GAATGC GAATGC(1/-1) BsoBI 992 CTCGAG C^[CT]CG[GA]G BsrI 587 CCAGT ACTGG(1/-1) BstF5I 841 CATCC GGATG(2/0) BstYI 401 AGATCT [GA]^GATC[CT] DraI 259 TTTAAA TTT^AAA DsaI 1014 CCATGG C^C[GA][CT]GG EarI 927 GAAGAG CTCTTC(1/4) EcoRV 307 GATATC GAT^ATC FbaI 300 TGATCA T^GATCA FokI 841 CATCC GGATG(9/13) HapII 786 CCGG C^CGG HpaII 786 CCGG C^CGG Ksp632I 927 GAAGAG CTCTTC(1/4) MflI 401 AGATCT [GA]^GATC[CT] MslI 518 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 603 CAGCTG C[AC]G^C[GT]G MspI 786 CCGG C^CGG NcoI 1014 CCATGG C^CATGG NdeI 645 CATATG CA^TATG PaeR7I 992 CTCGAG C^TCGAG PflMI 850 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 723 GAGTC GAGTC(4/5) PvuII 603 CAGCTG CAG^CTG SexAI 855 ACCTGGT A^CC[AT]GGT SfaNI 285 GCATC GCATC(5/9) SmlI 992 CTCGAG C^T[CT][GA]AG SspI 360 AATATT AAT^ATT StuI 931 AGGCCT AGG^CCT Van91I 850 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 397 CCAAAGATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 992 CTCGAG C^TCGAG XhoII 401 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 892 CCGC CCGC(-3/-1) 976 CCGC BanI 103 GGTGCC G^G[CT][GA]CC 873 GGCACC BbvI 659 GCAGC GCAGC(8/12) 1021 GCAGC BfaI 137 CTAG C^TAG 878 CTAG BsmFI 887 GTCCC GGGAC(10/14) 1038 GTCCC Cac8I 25 GCCAGC GC[ACGT]^[ACGT]GC 974 GCCCGC DpnII 301 GATC ^GATC 402 GATC FauI 891 CCCGC CCCGC(4/6) 975 CCCGC Fnu4HI 659 GCAGC GC^[ACGT]GC 1021 GCAGC HphI 701 TCACC GGTGA(8/7) 960 TCACC ItaI 659 GCAGC GC^[ACGT]GC 1021 GCAGC MaeI 137 CTAG C^TAG 878 CTAG MboI 301 GATC ^GATC 402 GATC NdeII 301 GATC ^GATC 402 GATC NlaIII 11 CATG CATG^ 1015 CATG Sau3AI 301 GATC ^GATC 402 GATC TaqI 593 TCGA T^CGA 993 TCGA TseI 659 GCAGC G^C[AT]GC 1021 GCAGC TspRI 588 CAGTG CA[GC]TG[ACGT][ACGT]^ 683 CACTG TthHB8I 593 TCGA T^CGA 993 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AcsI AluI ApoI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cfr13I DdeI EcoRII EcoT14I HaeIII HinfI MboII MnlI MseI MvaI NlaIV Sau96I ScrFI StyI TfiI Tru9I Tsp509I