(Linear) (Six-base) MAPSORT of: KIEE1477.seq Check: 9815 from: 1 to: 1053 KIEE1477.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 310 794 1053 Size: 170 140 484 259 Fragments arranged by size: 484 259 170 140 AleI CACnn'nnGTG Cuts at: 0 522 1053 Size: 522 531 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 893 925 1053 Size: 893 32 128 Fragments arranged by size: 893 128 32 ApaLI G'TGCA_C Cuts at: 0 555 1053 Size: 555 498 ApoI r'AATT_y Cuts at: 0 16 547 800 1053 Size: 16 531 253 253 Fragments arranged by size: 531 253 253 16 AvaI C'yCGr_G Cuts at: 0 992 1053 Size: 992 61 BanI G'GyrC_C Cuts at: 0 103 873 1053 Size: 103 770 180 Fragments arranged by size: 770 180 103 BanII G_rGCy'C Cuts at: 0 759 1053 Size: 759 294 BbsI GAAGACnn'nnnn_ Cuts at: 0 298 1053 Size: 298 755 BclI T'GATC_A Cuts at: 0 300 1053 Size: 300 753 BglII A'GATC_T Cuts at: 0 401 1053 Size: 401 652 Bme1580I G_kGCm'C Cuts at: 0 559 952 1053 Size: 559 393 101 Fragments arranged by size: 559 393 101 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 405 679 1053 Size: 405 274 374 Fragments arranged by size: 405 374 274 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 1053 Size: 766 287 BsaI GGTCTCn'nnnn_ Cuts at: 0 393 1053 Size: 393 660 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 668 735 754 836 1053 Size: 668 67 19 82 217 Fragments arranged by size: 668 217 82 67 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 83 1053 Size: 83 970 BsiHKAI G_wGCw'C Cuts at: 0 559 1053 Size: 559 494 BsmI GAATG_Cn' Cuts at: 0 90 1053 Size: 90 963 Bsp1286I G_dGCh'C Cuts at: 0 559 759 952 1053 Size: 559 200 193 101 Fragments arranged by size: 559 200 193 101 BstYI r'GATC_y Cuts at: 0 401 1053 Size: 401 652 BtgI C'CryG_G Cuts at: 0 1014 1053 Size: 1014 39 BtsI GCAGTG_nn' Cuts at: 0 682 1053 Size: 682 371 DraI TTT'AAA Cuts at: 0 261 1053 Size: 261 792 EarI CTCTTCn'nnn_ Cuts at: 0 922 1053 Size: 922 131 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 170 310 766 794 1053 Size: 170 140 456 28 259 Fragments arranged by size: 456 259 170 140 28 EcoRV GAT'ATC Cuts at: 0 309 1053 Size: 309 744 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 285 317 1053 Size: 285 32 736 Fragments arranged by size: 736 285 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 332 706 1053 Size: 332 374 347 Fragments arranged by size: 374 347 332 MslI CAynn'nnrTG Cuts at: 0 522 1053 Size: 522 531 MspA1I CmG'CkG Cuts at: 0 605 1053 Size: 605 448 NcoI C'CATG_G Cuts at: 0 1014 1053 Size: 1014 39 NdeI CA'TA_TG Cuts at: 0 646 1053 Size: 646 407 PflMI CCAn_nnn'nTGG Cuts at: 0 856 1053 Size: 856 197 PfoI T'CCnGG_A Cuts at: 0 406 897 1053 Size: 406 491 156 Fragments arranged by size: 491 406 156 PspXI vC'TCGA_Gb Cuts at: 0 992 1053 Size: 992 61 PvuII CAG'CTG Cuts at: 0 605 1053 Size: 605 448 SexAI A'CCwGG_T Cuts at: 0 855 1053 Size: 855 198 SmlI C'TyrA_G Cuts at: 0 992 1053 Size: 992 61 SspI AAT'ATT Cuts at: 0 362 1053 Size: 362 691 StuI AGG'CCT Cuts at: 0 933 1053 Size: 933 120 StyI C'CwwG_G Cuts at: 0 89 107 1014 1053 Size: 89 18 907 39 Fragments arranged by size: 907 89 39 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 310 1053 Size: 310 743 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 332 364 1053 Size: 332 32 689 Fragments arranged by size: 689 332 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 404 1053 Size: 404 649 XhoI C'TCGA_G Cuts at: 0 992 1053 Size: 992 61 Enzymes that do cut: AcuI AleI AloI ApaLI ApoI AvaI BanI BanII BbsI BclI BglII Bme1580I BplI BpmI BsaI BseRI BsgI BsiHKAI BsmI Bsp1286I BstYI BtgI BtsI DraI EarI Eco57MI EcoRV Hin4I MmeI MslI MspA1I NcoI NdeI PflMI PfoI PspXI PvuII SexAI SmlI SspI StuI StyI TaqII TstI XcmI XhoI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI Tth111I XbaI XmaI XmnI ZraI