Restriction map for
pFN21AB0937
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI Hin1I HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 336 GTAC GT^AC
AlwI 309 GATCC GGATC(4/5)
AlwNI 355 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 80 AGCGCT AGC^GCT
AspHI 61 GAGCTC G[AT]GC[AT]^C
AviII 266 TGCGCA TGC^GCA
BalI 209 TGGCCA TGG^CCA
BanI 46 GGCACC G^G[CT][GA]CC
BbvCI 420 CCTCAGC CCTCAGC(-5/-2)
BglI 253 GCCTTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 300 GCTGAGC GC^T[ACGT]AGC
BpmI 12 CTCCAG CTGGAG(16/14)
Bpu1102I 300 GCTGAGC GC^T[ACGT]AGC
BsaAI 403 TACGTG [CT]AC^GT[GA]
BsiEI 87 CGGCCG CG[GA][CT]^CG
BsiHKAI 61 GAGCTC G[AT]GC[AT]^C
BsmFI 27 GTCCC GGGAC(10/14)
BsrI 237 CCAGT ACTGG(1/-1)
BssHII 170 GCGCGC G^CGCGC
BssSI 412 CACGAG CACGAG(-5/-1)
BstYI 308 AGATCC [GA]^GATC[CT]
CelII 300 GCTGAGC GC^T[ACGT]AGC
DraII 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 32 CCGTGG C^C[GA][CT]GG
EagI 87 CGGCCG C^GGCCG
EclXI 87 CGGCCG C^GGCCG
Eco47III 80 AGCGCT AGC^GCT
Eco52I 87 CGGCCG C^GGCCG
EcoNI 348 CCTCTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 356 AGGTCCT [GA]G^G[ACGT]CC[CT]
FspI 266 TGCGCA TGC^GCA
HgaI 315 GACGC GACGC(5/10)
MboII 121 GAAGA GAAGA(8/7)
MflI 308 AGATCC [GA]^GATC[CT]
MluNI 209 TGGCCA TGG^CCA
MscI 209 TGGCCA TGG^CCA
MspA1I 393 CAGCTG C[AC]G^C[GT]G
PaeR7I 64 CTCGAG C^TCGAG
PpuMI 356 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 393 CAGCTG CAG^CTG
RsaI 336 GTAC GT^AC
SacI 61 GAGCTC GAGCT^C
SexAI 353 ACCAGGT A^CC[AT]GGT
SfaNI 203 GCATC GCATC(5/9)
SmaI 138 CCCGGG CCC^GGG
StuI 251 AGGCCT AGG^CCT
XhoI 64 CTCGAG C^TCGAG
XhoII 308 AGATCC [GA]^GATC[CT]
XmaCI 138 CCCGGG C^CCGGG
XmaI 138 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AvaI 64 CTCGAG C^[CT]CG[GA]G
138 CCCGGG
AvaII 357 GGTCC G^G[AT]CC
461 GGTCC
BanII 9 GGGCTC G[GA]GC[CT]^C
61 GAGCTC
BcnI 138 CCCGG CC^[GC]GG
139 CCGGG
BsoBI 64 CTCGAG C^[CT]CG[GA]G
138 CCCGGG
BstF5I 204 CATCC GGATG(2/0)
276 CATCC
DdeI 301 CTGAG C^T[ACGT]AG
421 CTCAG
DpnII 273 GATC ^GATC
309 GATC
FokI 204 CATCC GGATG(9/13)
276 CATCC
HapII 90 CCGG C^CGG
139 CCGG
HpaII 90 CCGG C^CGG
139 CCGG
MaeII 215 ACGT A^CGT
404 ACGT
MboI 273 GATC ^GATC
309 GATC
MspI 90 CCGG C^CGG
139 CCGG
NciI 138 CCCGG CC^[GC]GG
139 CCGGG
NdeII 273 GATC ^GATC
309 GATC
NlaIII 447 CATG CATG^
465 CATG
NlaIV 10 GGCTCC GG[ACGT]^[ACGT]CC
46 GGCACC
Sau3AI 273 GATC ^GATC
309 GATC
SmlI 64 CTCGAG C^T[CT][GA]AG
319 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EaeI EcoRII Fnu4HI HaeII
HaeIII HhaI HinP1I ItaI MnlI
MvaI MvnI MwoI PstI Sau96I
ScrFI SfcI TaqI TseI TthHB8I