Restriction map for
pFN21AB0937
(Linear) (Six-base) MAPSORT of: KIBB0937.seq Check: 3536 from: 1 to: 480
KIBB0937.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AfeI AGC'GCT
Cuts at: 0 82 480
Size: 82 398
AlwNI CAG_nnn'CTG
Cuts at: 0 360 480
Size: 360 120
AvaI C'yCGr_G
Cuts at: 0 64 138 480
Size: 64 74 342
Fragments arranged by size:
342 74 64
BanI G'GyrC_C
Cuts at: 0 46 480
Size: 46 434
BanII G_rGCy'C
Cuts at: 0 13 65 480
Size: 13 52 415
Fragments arranged by size:
415 52 13
BbvCI CC'TCA_GC
Cuts at: 0 421 480
Size: 421 59
BglI GCCn_nnn'nGGC
Cuts at: 0 259 480
Size: 259 221
BlpI GC'TnA_GC
Cuts at: 0 301 480
Size: 301 179
Bme1580I G_kGCm'C
Cuts at: 0 49 480
Size: 49 431
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 480
Size: 231 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 480
Size: 27 453
Bpu10I CC'TnA_GC
Cuts at: 0 421 480
Size: 421 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 480
Size: 304 176
BsaAI yAC'GTr
Cuts at: 0 405 480
Size: 405 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 442 448 472 480
Size: 418 24 6 24 8
Fragments arranged by size:
418 24 24 8 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 70 73 199 480
Size: 19 51 3 126 281
Fragments arranged by size:
281 126 51 19 3
BseYI C'CCAG_C
Cuts at: 0 166 480
Size: 166 314
BsiEI CG_ry'CG
Cuts at: 0 90 480
Size: 90 390
BsiHKAI G_wGCw'C
Cuts at: 0 65 480
Size: 65 415
Bsp1286I G_dGCh'C
Cuts at: 0 13 49 65 480
Size: 13 36 16 415
Fragments arranged by size:
415 36 16 13
BssHII G'CGCG_C
Cuts at: 0 170 480
Size: 170 310
BssSI C'ACGA_G
Cuts at: 0 412 480
Size: 412 68
BstYI r'GATC_y
Cuts at: 0 308 480
Size: 308 172
BtgI C'CryG_G
Cuts at: 0 32 480
Size: 32 448
EaeI y'GGCC_r
Cuts at: 0 87 209 326 480
Size: 87 122 117 154
Fragments arranged by size:
154 122 117 87
EagI C'GGCC_G
Cuts at: 0 87 480
Size: 87 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 459 480
Size: 459 21
EcoICRI GAG'CTC
Cuts at: 0 63 480
Size: 63 417
EcoNI CCTnn'n_nnAGG
Cuts at: 0 352 480
Size: 352 128
EcoO109I rG'GnC_Cy
Cuts at: 0 357 480
Size: 357 123
FspI TGC'GCA
Cuts at: 0 268 480
Size: 268 212
HaeII r_GCGC'y
Cuts at: 0 84 102 228 265 420 480
Size: 84 18 126 37 155 60
Fragments arranged by size:
155 126 84 60 37 18
MscI TGG'CCA
Cuts at: 0 211 480
Size: 211 269
MspA1I CmG'CkG
Cuts at: 0 395 480
Size: 395 85
PpuMI rG'GwC_Cy
Cuts at: 0 357 480
Size: 357 123
PstI C_TGCA'G
Cuts at: 0 194 365 400 480
Size: 194 171 35 80
Fragments arranged by size:
194 171 80 35
PvuII CAG'CTG
Cuts at: 0 395 480
Size: 395 85
SacI G_AGCT'C
Cuts at: 0 65 480
Size: 65 415
SexAI A'CCwGG_T
Cuts at: 0 353 480
Size: 353 127
SfcI C'TryA_G
Cuts at: 0 102 190 361 396 480
Size: 102 88 171 35 84
Fragments arranged by size:
171 102 88 84 35
SmaI CCC'GGG
Cuts at: 0 140 480
Size: 140 340
SmlI C'TyrA_G
Cuts at: 0 64 319 480
Size: 64 255 161
Fragments arranged by size:
255 161 64
StuI AGG'CCT
Cuts at: 0 253 480
Size: 253 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 480
Size: 250 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 480
Size: 442 32 6
Fragments arranged by size:
442 32 6
XhoI C'TCGA_G
Cuts at: 0 64 480
Size: 64 416
XmaI C'CCGG_G
Cuts at: 0 138 480
Size: 138 342
Enzymes that do cut:
AfeI AlwNI AvaI BanI BanII BbvCI BglI BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BssHII BssSI BstYI BtgI
EaeI EagI EciI EcoICRI EcoNI EcoO109I FspI HaeII
MscI MspA1I PpuMI PstI PvuII SacI SexAI SfcI
SmaI SmlI StuI TaqII TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpmI
BsaI BsaBI BsaHI BsaWI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI Eco57MI EcoRI EcoRV
FalI FseI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
Tth111I XbaI XcmI XmnI ZraI