(Circular) (Six-base) MAPSORT of: pFN21AE6354.seq Check: 8074 from: 1 to: 5860 pFN21AE6354 5860 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2054 3670 278 Size: 53 83 186 936 518 1616 2468 Fragments arranged by size: 2468 1616 936 518 186 83 53 AccI GT'mk_AC Cuts at: 1937 2298 3203 1937 Size: 361 905 4594 Fragments arranged by size: 4594 905 361 Acc65I G'GTAC_C Cuts at: 2577 2970 3181 2577 Size: 393 211 5256 Fragments arranged by size: 5256 393 211 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2307 3923 5307 5575 1632 Size: 675 1616 1384 268 1917 Fragments arranged by size: 1917 1616 1384 675 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 2415 1166 Size: 1249 4611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2684 1184 Size: 1500 4360 AlwNI CAG_nnn'CTG Cuts at: 2248 2285 3088 3603 5175 2248 Size: 37 803 515 1572 2933 Fragments arranged by size: 2933 1572 803 515 37 ApaI G_GGCC'C Cuts at: 2587 2587 Size: 5860 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2134 3185 1870 Size: 73 191 1051 4545 Fragments arranged by size: 4545 1051 191 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2072 2423 2577 2970 Size: 325 217 627 229 56 351 154 393 Cuts at: 2970 3181 4636 618 Size: 211 1455 1842 Fragments arranged by size: 1842 1455 627 393 351 325 229 217 211 154 56 BanII G_rGCy'C Cuts at: 1391 2587 2943 3179 1391 Size: 1196 356 236 4072 Fragments arranged by size: 4072 1196 356 236 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4889 BbvCI CC'TCA_GC Cuts at: 2099 3140 2099 Size: 1041 4819 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BfrBI ATG'CAT Cuts at: 2569 2569 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 2688 4471 Size: 107 122 71 631 605 466 550 1783 Cuts at: 4471 136 Size: 1525 Fragments arranged by size: 1783 1525 631 605 550 466 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2077 2587 2966 3921 5077 1790 Size: 287 510 379 955 1156 2573 Fragments arranged by size: 2573 1156 955 510 379 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2273 2434 3591 Size: 416 53 83 399 24 856 161 1157 Cuts at: 3591 4549 442 Size: 958 1753 Fragments arranged by size: 1753 1157 958 856 416 399 161 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2540 2955 4520 1239 Size: 1301 415 1565 2579 Fragments arranged by size: 2579 1565 1301 415 Bpu10I CC'TnA_GC Cuts at: 2099 3140 2099 Size: 1041 4819 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 2624 2636 3985 4850 5148 2618 Size: 6 12 1349 865 298 3330 Fragments arranged by size: 3330 1349 865 298 12 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2261 3046 4523 882 Size: 617 762 785 1477 2219 Fragments arranged by size: 2219 1477 785 762 617 BsaAI yAC'GTr Cuts at: 493 1204 2668 2967 3651 493 Size: 711 1464 299 684 2702 Fragments arranged by size: 2702 1464 711 684 299 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2051 3667 4049 4684 Size: 53 83 186 936 518 1616 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1616 1451 936 635 518 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2775 4293 4691 4965 5112 5523 2775 Size: 1518 398 274 147 411 3112 Fragments arranged by size: 3112 1518 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2951 2981 1178 Size: 30 165 30 1548 30 4057 Fragments arranged by size: 4057 1548 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2621 3034 1186 Size: 1435 413 4012 Fragments arranged by size: 4012 1435 413 BseYI C'CCAG_C Cuts at: 2115 2586 2736 2991 5063 5559 2115 Size: 471 150 255 2072 496 2416 Fragments arranged by size: 2416 2072 496 471 255 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2707 3018 1163 Size: 573 971 311 4005 Fragments arranged by size: 4005 971 573 311 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2556 3921 4006 5077 2556 Size: 1365 85 1071 3339 Fragments arranged by size: 3339 1365 1071 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2556 2587 2943 2966 3179 3921 Size: 399 287 479 31 356 23 213 742 Cuts at: 3921 4006 5077 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 479 399 356 287 213 85 31 23 BspEI T'CCGG_A Cuts at: 2775 5523 2775 Size: 2748 3112 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2565 3215 844 Size: 1255 466 650 3489 Fragments arranged by size: 3489 1255 650 466 BsrBI CCG'CTC Cuts at: 1394 1823 2310 3747 1394 Size: 429 487 1437 3507 Fragments arranged by size: 3507 1437 487 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2430 4355 4529 65 Size: 1183 1182 1925 174 1396 Fragments arranged by size: 1925 1396 1183 1182 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2069 2208 2420 4504 4691 1700 Size: 110 259 139 212 2084 187 2869 Fragments arranged by size: 2869 2084 259 212 187 139 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 2030 1925 Size: 105 5755 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstYI r'GATC_y Cuts at: 1972 3060 3190 3557 3942 3959 5411 5497 5509 Size: 1088 130 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1088 385 367 346 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2345 2345 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 2004 2262 2361 2510 2955 513 Size: 552 939 258 99 149 445 3418 Fragments arranged by size: 3418 939 552 445 258 149 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2246 2461 3131 3376 4170 4190 2148 Size: 98 215 670 245 794 20 3818 Fragments arranged by size: 3818 794 670 245 215 98 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2171 4867 809 Size: 507 855 2696 1802 Fragments arranged by size: 2696 1802 855 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825 Size: 54 1425 50 275 18 1477 889 1629 Cuts at: 5825 8 Size: 43 Fragments arranged by size: 1629 1477 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3005 3790 1353 Size: 1652 785 3423 Fragments arranged by size: 3423 1652 785 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2540 2955 3923 4520 5307 5575 Size: 393 675 233 415 968 597 787 268 Cuts at: 5575 1239 Size: 1524 Fragments arranged by size: 1524 968 787 675 597 415 393 268 233 EcoO109I rG'GnC_Cy Cuts at: 2335 2350 3019 3085 3111 2335 Size: 15 669 66 26 5084 Fragments arranged by size: 5084 669 66 26 15 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2044 2144 5007 1678 Size: 366 100 2863 2531 Fragments arranged by size: 2863 2531 366 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373 Size: 32 135 32 2929 32 42 32 2626 Fragments arranged by size: 2929 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2299 3204 3450 669 Size: 988 281 361 905 246 3079 Fragments arranged by size: 3079 988 905 361 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 2581 2974 3185 2581 Size: 393 211 5256 Fragments arranged by size: 5256 393 211 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4974 5158 1305 Size: 3669 184 2007 Fragments arranged by size: 3669 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4038 Fragments arranged by size: 4038 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2415 3818 4177 4336 518 Size: 648 1249 1403 359 159 2042 Fragments arranged by size: 2042 1403 1249 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2590 2721 3276 3953 5101 5346 1606 Size: 984 131 555 677 1148 245 2120 Fragments arranged by size: 2120 1148 984 677 555 245 131 NaeI GCC'GGC Cuts at: 2210 2422 2210 Size: 212 5648 NcoI C'CATG_G Cuts at: 513 1065 2004 2262 2955 513 Size: 552 939 258 693 3418 Fragments arranged by size: 3418 939 693 552 258 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NgoMIV G'CCGG_C Cuts at: 2208 2420 2208 Size: 212 5648 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NsiI A_TGCA'T Cuts at: 2571 2571 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2764 3218 4763 1132 Size: 97 1535 454 1545 2229 Fragments arranged by size: 2229 1545 1535 454 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 3107 3107 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PmlI CAC'GTG Cuts at: 2967 2967 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2335 2350 3111 2335 Size: 15 761 5084 Fragments arranged by size: 5084 761 15 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2583 2583 Size: 5860 PstI C_TGCA'G Cuts at: 830 2110 3212 830 Size: 1280 1102 3478 Fragments arranged by size: 3478 1280 1102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 SalI G'TCGA_C Cuts at: 1936 2297 3202 1936 Size: 361 905 4594 Fragments arranged by size: 4594 905 361 SanDI GG'GwC_CC Cuts at: 2350 2350 Size: 5860 SapI GCTCTTCn'nnn_ Cuts at: 3005 3005 Size: 5860 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SexAI A'CCwGG_T Cuts at: 2973 2973 Size: 5860 SfcI C'TryA_G Cuts at: 826 1045 2106 3035 3208 4343 5024 5215 826 Size: 219 1061 929 173 1135 681 191 1471 Fragments arranged by size: 1471 1135 1061 929 681 219 191 173 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 2069 1700 Size: 369 5491 SmaI CCC'GGG Cuts at: 1872 2136 3187 1872 Size: 264 1051 4545 Fragments arranged by size: 4545 1051 264 SmlI C'TyrA_G Cuts at: 820 1017 1943 2633 2639 2651 3964 4865 5127 Size: 197 926 690 6 12 1313 901 262 Cuts at: 5127 820 Size: 1553 Fragments arranged by size: 1553 1313 926 901 690 262 197 12 6 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 2262 2748 2943 2955 3299 Size: 552 939 183 75 486 195 12 344 Cuts at: 3299 513 Size: 3074 Fragments arranged by size: 3074 939 552 486 344 195 183 75 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2255 2434 2593 2918 3040 3887 3904 Size: 1255 675 179 159 325 122 847 17 Cuts at: 3904 4057 4242 4687 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 847 675 445 325 185 179 159 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 4106 5818 Size: 274 80 33 51 1443 678 1451 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1451 1443 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2140 2172 2951 2983 5820 5852 2140 Size: 32 779 32 2837 32 2148 Fragments arranged by size: 2837 2148 779 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 2134 3185 1870 Size: 264 1051 4545 Fragments arranged by size: 4545 1051 264 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2052 3668 276 Size: 53 83 186 936 518 1616 2468 Fragments arranged by size: 2468 1616 936 518 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI