(Circular) (Six-base) MAPSORT of: pFN21AB5887.seq Check: 8652 from: 1 to: 6196 pFN21AB5887 6196 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2318 3539 1937 Size: 381 1221 4594 Fragments arranged by size: 4594 1221 381 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3336 3450 4259 5643 5911 1632 Size: 1704 114 809 1384 268 1917 Fragments arranged by size: 1917 1704 1384 809 268 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 5095 6072 1203 Size: 3892 977 1327 Fragments arranged by size: 3892 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 3407 4925 3407 Size: 1518 4678 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6196 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AlwNI CAG_nnn'CTG Cuts at: 3939 5511 3939 Size: 1572 4624 ApaLI G'TGCA_C Cuts at: 4253 5409 4253 Size: 1156 5040 ApoI r'AATT_y Cuts at: 1493 2069 2323 2749 2758 3192 3483 3505 3731 Size: 576 254 426 9 434 291 22 226 Cuts at: 3731 3883 5992 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 576 434 426 291 254 226 152 22 9 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 2467 3521 1870 Size: 73 524 1054 4545 Fragments arranged by size: 4545 1054 524 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2025 2058 2025 Size: 33 6163 BamHI G'GATC_C Cuts at: 3526 3893 3526 Size: 367 5829 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3330 3517 4972 618 Size: 325 217 627 1543 187 1455 1842 Fragments arranged by size: 1842 1543 1455 627 325 217 187 BanII G_rGCy'C Cuts at: 1391 3515 1391 Size: 2124 4072 BbeI G_GCGC'C Cuts at: 3334 3334 Size: 6196 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2254 2952 928 Size: 971 355 698 4172 Fragments arranged by size: 4172 971 698 355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2272 2342 2985 4088 5298 2272 Size: 70 643 1103 1210 3170 Fragments arranged by size: 3170 1210 1103 643 70 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3241 6191 3241 Size: 2950 3246 BlpI GC'TnA_GC Cuts at: 3613 3613 Size: 6196 Bme1580I G_kGCm'C Cuts at: 1790 4257 5413 1790 Size: 2467 1156 2573 Fragments arranged by size: 2573 2467 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2442 2934 3927 Size: 416 53 83 399 24 1025 492 993 Cuts at: 3927 4885 442 Size: 958 1753 Fragments arranged by size: 1753 1025 993 958 492 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4856 1239 Size: 3617 2579 Bpu10I CC'TnA_GC Cuts at: 3326 3326 Size: 6196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3068 3245 4321 5186 5484 3068 Size: 177 1076 865 298 3780 Fragments arranged by size: 3780 1076 865 298 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4859 882 Size: 617 3360 2219 Fragments arranged by size: 3360 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3331 4003 4385 5020 Size: 53 83 186 936 1798 672 382 635 Cuts at: 5020 275 Size: 1451 Fragments arranged by size: 1798 1451 936 672 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4629 5027 5301 5448 5859 4629 Size: 398 274 147 411 4966 Fragments arranged by size: 4966 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2169 2199 1178 Size: 30 165 30 766 30 5175 Fragments arranged by size: 5175 766 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2931 3065 3356 1186 Size: 1745 134 291 4026 Fragments arranged by size: 4026 1745 291 134 BseYI C'CCAG_C Cuts at: 5399 5895 5399 Size: 496 5700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2618 1163 Size: 573 882 4741 Fragments arranged by size: 4741 882 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4257 4342 5413 4257 Size: 85 1071 5040 Fragments arranged by size: 5040 1071 85 BsmI GAATG_Cn' Cuts at: 3256 3707 3800 3256 Size: 451 93 5652 Fragments arranged by size: 5652 451 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3515 4257 4342 5413 1391 Size: 399 1725 742 85 1071 2174 Fragments arranged by size: 2174 1725 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 2428 3551 844 Size: 1209 375 1123 3489 Fragments arranged by size: 3489 1209 1123 375 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4691 4865 65 Size: 1183 3443 174 1396 Fragments arranged by size: 3443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4840 5027 1700 Size: 110 3030 187 2869 Fragments arranged by size: 3030 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 2148 4256 5268 2148 Size: 2108 1012 3076 Fragments arranged by size: 3076 2108 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6196 BstBI TT'CG_AA Cuts at: 2716 5000 2716 Size: 2284 3912 BstEII G'GTnAC_C Cuts at: 1184 2588 1184 Size: 1404 4792 BstYI r'GATC_y Cuts at: 1972 2027 2808 3241 3526 3893 4278 4295 5747 Size: 55 781 433 285 367 385 17 1452 Cuts at: 5747 5833 5845 6191 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 781 433 385 367 346 285 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2787 513 Size: 552 1722 3922 Fragments arranged by size: 3922 1722 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 2606 3712 4506 4526 2606 Size: 1106 794 20 4276 Fragments arranged by size: 4276 1106 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6161 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2522 3643 4532 Size: 54 1425 50 275 18 692 1121 889 Cuts at: 4532 6161 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1121 889 692 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 3312 4126 1353 Size: 720 1239 814 3423 Fragments arranged by size: 3423 1239 814 720 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3336 3450 4259 4856 5643 5911 1239 Size: 393 1704 114 809 597 787 268 1524 Fragments arranged by size: 1704 1524 809 787 597 393 268 114 EcoNI CCTnn'n_nnAGG Cuts at: 2392 3338 2392 Size: 946 5250 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 EcoRV GAT'ATC Cuts at: 3201 3201 Size: 6196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3306 3306 Size: 6196 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 3334 5343 1678 Size: 1656 2009 2531 Fragments arranged by size: 2531 2009 1656 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 3393 3425 4837 Size: 32 135 32 812 32 977 32 1412 Cuts at: 4837 4869 4911 4943 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1412 977 812 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2842 3439 3540 3786 669 Size: 988 281 904 597 101 246 3079 Fragments arranged by size: 3079 988 904 597 281 246 101 HindIII A'AGCT_T Cuts at: 748 3347 748 Size: 2599 3597 HpaI GTT'AAC Cuts at: 2842 3786 2842 Size: 944 5252 KasI G'GCGC_C Cuts at: 3330 3330 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 5310 5494 1305 Size: 919 3086 184 2007 Fragments arranged by size: 3086 2007 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2524 10 Size: 54 1425 343 692 3682 Fragments arranged by size: 3682 1425 692 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4154 4513 4672 518 Size: 648 2988 359 159 2042 Fragments arranged by size: 2988 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2210 3612 4289 5437 5682 1606 Size: 604 1402 677 1148 245 2120 Fragments arranged by size: 2120 1402 1148 677 604 245 NarI GG'CG_CC Cuts at: 3331 3331 Size: 6196 NcoI C'CATG_G Cuts at: 513 1065 2787 513 Size: 552 1722 3922 Fragments arranged by size: 3922 1722 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2414 2966 3554 5099 1132 Size: 97 1185 552 588 1545 2229 Fragments arranged by size: 2229 1545 1185 588 552 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6196 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3444 1613 Size: 1831 4365 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 2050 3548 830 Size: 1220 1498 3478 Fragments arranged by size: 3478 1498 1220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 PvuII CAG'CTG Cuts at: 2210 2210 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SapI GCTCTTCn'nnn_ Cuts at: 3312 3312 Size: 6196 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 3313 4444 6156 3313 Size: 1131 1712 3353 Fragments arranged by size: 3353 1712 1131 SfcI C'TryA_G Cuts at: 826 1045 2046 2211 3544 4679 5360 5551 826 Size: 219 1001 165 1333 1135 681 191 1471 Fragments arranged by size: 1471 1333 1135 1001 681 219 191 165 SfoI GGC'GCC Cuts at: 3332 3332 Size: 6196 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3047 3224 4300 5201 5463 820 Size: 197 926 1104 177 1076 901 262 1553 Fragments arranged by size: 1553 1104 1076 926 901 262 197 177 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 2713 3028 3376 4120 5 Size: 47 2661 315 348 744 2081 Fragments arranged by size: 2661 2081 744 348 315 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2787 2951 3635 513 Size: 552 1722 164 684 3074 Fragments arranged by size: 3074 1722 684 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4223 4240 4393 4578 5023 325 Size: 1255 2643 17 153 185 445 1498 Fragments arranged by size: 2643 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2346 3311 4442 Size: 274 80 33 51 1443 369 965 1131 Cuts at: 4442 6154 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1131 965 369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2169 2201 2926 2958 6156 6188 2169 Size: 32 725 32 3198 32 2177 Fragments arranged by size: 3198 2177 725 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 3192 4325 3192 Size: 1133 5063 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI