################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeII MaeIII MfeI MflI MluI MluNI MroI MscI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 246 GGTACC G^GTACC AccI 638 GTCTAC GT^[AC][GT]AC AciI 427 GCGG CCGC(-3/-1) ApaI 121 GGGCCC GGGCC^C Asp718I 246 GGTACC G^GTACC BanII 121 GGGCCC G[GA]GC[CT]^C BcnI 36 CCCGG CC^[GC]GG BfaI 470 CTAG C^TAG BglI 170 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 121 GGGCCC G[AGT]GC[ACT]^C BsaBI 576 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 528 GAGACC GGTCTC(1/5) BsgI 708 GTGCAG GTGCAG(16/14) BsmAI 528 GAGAC GTCTC(1/5) Bsp1286I 121 GGGCCC G[AGT]GC[ACT]^C BsrFI 151 GCCGGC [GA]^CCGG[CT] CfoI 262 GCGC GCG^C Cfr10I 151 GCCGGC [GA]^CCGG[CT] DpnII 316 GATC ^GATC DsaI 250 CCATGG C^C[GA][CT]GG EaeI 153 CGGCCA [CT]^GGCC[GA] HaeII 261 AGCGCC [GA]GCGC^[CT] HgaI 817 GACGC GACGC(5/10) HhaI 262 GCGC GCG^C HinP1I 262 GCGC G^CGC HphI 33 TCACC GGTGA(8/7) KpnI 246 GGTACC GGTAC^C MaeI 470 CTAG C^TAG MamI 576 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 316 GATC ^GATC MboII 430 GAAGA GAAGA(8/7) MslI 533 CACTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 151 GCCGGC GCC^GGC NciI 36 CCCGG CC^[GC]GG NcoI 250 CCATGG C^CATGG NdeII 316 GATC ^GATC NgoMIV 151 GCCGGC G^CCGGC PflMI 779 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 239 CTGCAG CTGCA^G Sau3AI 316 GATC ^GATC SfaNI 255 GATGC GCATC(5/9) SfcI 239 CTGCAG C^T[GA][CT]AG SmlI 483 CTTGAG C^T[CT][GA]AG SspI 299 AATATT AAT^ATT Van91I 779 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 469 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 773 AAATTT [GA]^AATT[CT] 794 GAATTT ApoI 773 AAATTT [GA]^AATT[CT] 794 GAATTT BciVI 60 GTATCC GTATCC(6/5) 72 GTATCC BpmI 98 CTCCAG CTGGAG(16/14) 523 CTGGAG BseRI 65 CTCCTC GAGGAG(10/8) 590 CTCCTC BsiYI 145 CCACCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 779 CCAAGCACTGG BslI 145 CCACCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 779 CCAAGCACTGG BsmFI 187 GTCCC GGGAC(10/14) 557 GTCCC BsmI 24 GAATGC GAATGC(1/-1) 85 GCATTC BstF5I 254 GGATG GGATG(2/0) 675 GGATG DdeI 68 CTCAG C^T[ACGT]AG 535 CTAAG EcoT14I 90 CCAAGG C^C[AT][AT]GG 250 CCATGG FokI 254 GGATG GGATG(9/13) 675 GGATG HapII 37 CCGG C^CGG 152 CCGG HinfI 327 GATTC G^A[ACGT]TC 833 GAATC HpaII 37 CCGG C^CGG 152 CCGG MspI 37 CCGG C^CGG 152 CCGG MwoI 170 GCCCAGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 920 GCACTGGCAGC PpuMI 93 AGGACCT [GA]G^G[AT]CC[CT] 162 AGGTCCT StyI 90 CCAAGG C^C[AT][AT]GG 250 CCATGG TfiI 327 GATTC G^A[AT]TC 833 GAATC XcmI 156 CCATTCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 585 CCAAGCTCCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AluI AlwNI AvaII BanI BbvI BsaJI BsrI BssKI BstNI Cac8I Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII ItaI MnlI MseI MspA1I MvaI NlaIII NlaIV PvuII RsaI Sau96I ScrFI Tru9I TseI Tsp509I TspRI