################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MscI MseI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 95 TCCGGA T^CCGGA AflIII 117 ACATGT A^C[GA][CT]GT AlwNI 179 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 463 CCTCAGG CC^T[ACGT]AGG Aor51HI 326 AGCGCT AGC^GCT ApaI 14 GGGCCC GGGCC^C AspI 256 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 255 GGACC G^G[AT]CC BanII 14 GGGCCC G[GA]GC[CT]^C BcnI 300 CCCGG CC^[GC]GG BmyI 14 GGGCCC G[AGT]GC[ACT]^C BsaAI 136 TACGTG [CT]AC^GT[GA] BsaWI 95 TCCGGA [AT]^CCGG[AT] BseAI 95 TCCGGA T^CCGGA BseRI 223 GAGGAG GAGGAG(10/8) BsgI 121 GTGCAG GTGCAG(16/14) BsmAI 492 GAGAC GTCTC(1/5) Bsp1286I 14 GGGCCC G[AGT]GC[ACT]^C BspEI 95 TCCGGA T^CCGGA BspLU11I 117 ACATGT A^CATGT BsrFI 278 ACCGGC [GA]^CCGG[CT] BstAPI 178 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 396 CATCC GGATG(2/0) BstXI 565 CCACTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 463 CCTCAGG CC^T[ACGT]AGG CfoI 327 GCGC GCG^C Cfr10I 278 ACCGGC [GA]^CCGG[CT] DraII 14 GGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 169 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 188 CCATGG C^C[GA][CT]GG EaeI 44 TGGCCG [CT]^GGCC[GA] Eco47III 326 AGCGCT AGC^GCT Eco81I 463 CCTCAGG CC^T[ACGT]AGG EcoNI 345 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 14 GGGCCCC [GA]G^G[ACGT]CC[CT] FauI 519 GCGGG CCCGC(4/6) FokI 396 CATCC GGATG(9/13) HaeII 326 AGCGCT [GA]GCGC^[CT] HgaI 181 GACGC GACGC(5/10) HhaI 327 GCGC GCG^C HinP1I 327 GCGC G^CGC MaeII 137 ACGT A^CGT MaeIII 433 GTGAC ^GT[ACGT]AC MroI 95 TCCGGA T^CCGGA NciI 300 CCCGG CC^[GC]GG NcoI 188 CCATGG C^CATGG NspI 117 ACATGT [GA]CATG^[CT] PflFI 256 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 36 CTGCAG CTGCA^G SexAI 209 ACCTGGT A^CC[AT]GGT SfaNI 533 GCATC GCATC(5/9) SfcI 36 CTGCAG C^T[GA][CT]AG SmlI 420 CTTGAG C^T[CT][GA]AG Tsp45I 433 GTGAC ^GT[GC]AC Tth111I 256 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbsI 409 GAAGAC GAAGAC(2/6) 67 GTCTTC BpuAI 409 GAAGAC GAAGAC(2/6) 67 GTCTTC BsrI 246 CCAGT ACTGG(1/-1) 284 CCAGT Cac8I 239 GCCAGC GC[ACGT]^[ACGT]GC 266 GCTGGC DdeI 314 CTGAG C^T[ACGT]AG 464 CTCAG DpnII 55 GATC ^GATC 166 GATC EarI 559 CTCTTC CTCTTC(1/4) 489 GAAGAG EcoT14I 188 CCATGG C^C[AT][AT]GG 251 CCAAGG HphI 432 GGTGA GGTGA(8/7) 341 TCACC Ksp632I 559 CTCTTC CTCTTC(1/4) 489 GAAGAG MboI 55 GATC ^GATC 166 GATC MslI 114 CATACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 400 CACCAGCATG NdeII 55 GATC ^GATC 166 GATC NlaIV 14 GGGCCC GG[ACGT]^[ACGT]CC 15 GGCCCC PvuII 124 CAGCTG CAG^CTG 232 CAGCTG Sau3AI 55 GATC ^GATC 166 GATC StyI 188 CCATGG C^C[AT][AT]GG 251 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AfaI AluI BbvI BsaJI BsiYI BslI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspA1I MspI MvaI MwoI NlaIII RsaI Sau96I ScrFI TseI TspRI