Restriction map for pFN21AE3586
 (Circular) (Six-base) MAPSORT of: pFN21AE3586.seq  Check: 2315  from: 1  to: 5320



pFN21AE3586  5320 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 13:34  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3130     278 
   Size:         53      83     186     936    1594    2468

  Fragments arranged by size: 

                2468    1594     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2663    1937 
   Size:        726    4594
 
Acc65I G'GTAC_C

Cuts at:   2641    2641 
   Size:       5320
 
AclI AA'CG_TT

Cuts at:   1563    3447    3820    1563 
   Size:       1884     373    3063

  Fragments arranged by size: 

                3063    1884     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3383    4767    5035    1632 
   Size:       1751    1384     268    1917

  Fragments arranged by size: 

                1917    1751    1384     268
 
AfeI AGC'GCT

Cuts at:   2333    2333 
   Size:       5320
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5123
 
AflIII A'CryG_T

Cuts at:   1203    2122    4219    5196    1203 
   Size:        919    2097     977    1327

  Fragments arranged by size: 

                2097    1327     977     919
 
AgeI A'CCGG_T

Cuts at:   4151    4151 
   Size:       5320
 
AhdI GACnn_n'nnGTC

Cuts at:   4049    4049 
   Size:       5320
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5320
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5320
 
AlwNI CAG_nnn'CTG

Cuts at:   2189    3063    4635    2189 
   Size:        874    1572    2874

  Fragments arranged by size: 

                2874    1572     874
 
ApaI G_GGCC'C

Cuts at:   2023    2023 
   Size:       5320
 
ApaLI G'TGCA_C

Cuts at:   3377    4533    3377 
   Size:       1156    4164
 
ApoI r'AATT_y

Cuts at:   1493    2629    2855    3007    5116    1493 
   Size:       1136     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1136     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3874     160 
   Size:        872    2842    1606

  Fragments arranged by size: 

                2842    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5320
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2645    1870 
   Size:         73     702    4545

  Fragments arranged by size: 

                4545     702      73
 
BamHI G'GATC_C

Cuts at:   2650    3017    2650 
   Size:        367    4953
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2641    4096     618 
   Size:        325     217     627     854    1455    1842

  Fragments arranged by size: 

                1842    1455     854     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2023    2639    1391 
   Size:        632     616    4072

  Fragments arranged by size: 

                4072     632     616
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2065    2421     928 
   Size:        971     166     356    3827

  Fragments arranged by size: 

                3827     971     356     166
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3511    3545    1903 
   Size:         34    1574      34    3678

  Fragments arranged by size: 

                3678    1574      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3212    4422    3212 
   Size:       1210    4110
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5320
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3931     136 
   Size:        107     122      71     631     605    2259    1525

  Fragments arranged by size: 

                2259    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5315    5315 
   Size:       5320
 
BlpI GC'TnA_GC

Cuts at:   2737    2737 
   Size:       5320
 
Bme1580I G_kGCm'C

Cuts at:   1790    2023    3381    4537    1790 
   Size:        233    1358    1156    2573

  Fragments arranged by size: 

                2573    1358    1156     233
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5320
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2245    2283    3051 
   Size:        416      53      83     399      24     828      38     768

Cuts at:   3051    4009     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753     958     828     768     416     399      83      53
                  38      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5320
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3980    1239 
   Size:       2741    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2446    3445    4310    4608    2446 
   Size:        999     865     298    3158

  Fragments arranged by size: 

                3158     999     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3983     882 
   Size:        617    2484    2219

  Fragments arranged by size: 

                2484    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2143    3111     493 
   Size:        711     939     968    2702

  Fragments arranged by size: 

                2702     968     939     711
 
BsaBI GATnn'nnATC

Cuts at:   5314    5314 
   Size:       5320
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3127    3509    4144     275 
   Size:         53      83     186     936    1594     382     635    1451

  Fragments arranged by size: 

                1594    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2100    3753    4151    4425    4572    4983    2100 
   Size:       1653     398     274     147     411    2437

  Fragments arranged by size: 

                2437    1653     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2300    2330    2375    2405    1178 
   Size:         30     165      30     897      30      45      30    4093

  Fragments arranged by size: 

                4093     897     165      45      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2243    1186 
   Size:       1057    4263
 
BseYI C'CCAG_C

Cuts at:   4523    5019    4523 
   Size:        496    4824
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2147    1163 
   Size:        573     411    4336

  Fragments arranged by size: 

                4336     573     411
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2770    3531    4559    1540 
   Size:        275     186     769     761    1028    2301

  Fragments arranged by size: 

                2301    1028     769     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3381    3466    4537    3381 
   Size:         85    1071    4164

  Fragments arranged by size: 

                4164    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2831    2924    2831 
   Size:         93    5227
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2023    2639    3381    3466    4537    1391 
   Size:        399     233     616     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     616     399     233      85
 
BspEI T'CCGG_A

Cuts at:   2100    4983    2100 
   Size:       2883    2437
 
BspHI T'CATG_A

Cuts at:   3209    4939    3209 
   Size:       1730    3590
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2675     844 
   Size:       1831    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3207    1394 
   Size:        429    1384    3507

  Fragments arranged by size: 

                3507    1384     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3815    3989      65 
   Size:       1183    2567     174    1396

  Fragments arranged by size: 

                2567    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2283    3964    4151    1700 
   Size:        110     473    1681     187    2869

  Fragments arranged by size: 

                2869    1681     473     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5320
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5320
 
BssSI C'ACGA_G

Cuts at:   3380    4392    3380 
   Size:       1012    4308
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2189     794 
   Size:       1395    3925
 
BstBI TT'CG_AA

Cuts at:   4124    4124 
   Size:       5320
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5320
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2577    2577 
   Size:       5320
 
BstYI r'GATC_y

Cuts at:   1972    2650    3017    3402    3419    4871    4957    4969    5315 
   Size:        678     367     385      17    1452      86      12     346

Cuts at:   5315    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     678     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2469    2469 
   Size:       5320
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2193     513 
   Size:        552     939     189    3640

  Fragments arranged by size: 

                3640     939     552     189
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2524     487 
   Size:        683      56    1298    3283

  Fragments arranged by size: 

                3283    1298     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2282    2836    3630    3650    2282 
   Size:        554     794      20    3952

  Fragments arranged by size: 

                3952     794     554      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5320
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5285
 
DraI TTT'AAA

Cuts at:   2624    2624 
   Size:       5320
 
DraIII CAC_nnn'GTG

Cuts at:   2179    2179 
   Size:       5320
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4327     809 
   Size:        507    3011    1802

  Fragments arranged by size: 

                3011    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2049    2767    3656 
   Size:         54    1425      50     275      18     219     718     889

Cuts at:   3656    5285       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     718     275     219      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2767    1537 
   Size:        275     955    4090

  Fragments arranged by size: 

                4090     955     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2489    2570    3250    1353 
   Size:       1136      81     680    3423

  Fragments arranged by size: 

                3423    1136     680      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3907    4281    4427     387 
   Size:       1064     138    2318     374     146    1280

  Fragments arranged by size: 

                2318    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3383    3980    4767    5035    1239 
   Size:        393    1751     597     787     268    1524

  Fragments arranged by size: 

                1751    1524     787     597     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2354    2354 
   Size:       5320
 
EcoO109I rG'GnC_Cy

Cuts at:   2020    2020 
   Size:       5320
 
EcoRI G'AATT_C

Cuts at:   2629    3007    2629 
   Size:        378    4942
 
FspI TGC'GCA

Cuts at:   3826    3826 
   Size:       5320
 
HaeII r_GCGC'y

Cuts at:   1678    2335    4467    1678 
   Size:        657    2132    2531

  Fragments arranged by size: 

                2531    2132     657
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2300    2332    3961    3993    4035 
   Size:         32     135      32     728      32    1629      32      42

Cuts at:   4035    4067    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1629     728     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2664    2910     669 
   Size:        988     281     726     246    3079

  Fragments arranged by size: 

                3079     988     726     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5320
 
HpaI GTT'AAC

Cuts at:   2910    2910 
   Size:       5320
 
KpnI G_GTAC'C

Cuts at:   2645    2645 
   Size:       5320
 
MfeI C'AATT_G

Cuts at:   2919    2919 
   Size:       5320
 
MluI A'CGCG_T

Cuts at:   5196    5196 
   Size:       5320
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4434    4618    1305 
   Size:       3129     184    2007

  Fragments arranged by size: 

                3129    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3498

  Fragments arranged by size: 

                3498    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2123    2409    3278    3637    3796     518 
   Size:        648     957     286     869     359     159    2042

  Fragments arranged by size: 

                2042     957     869     648     359     286     159
 
MspA1I CmG'CkG

Cuts at:   1606    2054    2131    2239    2271    2421    2736    3413    4561 
   Size:        448      77     108      32     150     315     677    1148

Cuts at:   4561    4806    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1148     677     448     315     245     150     108
                  77      32
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2193     513 
   Size:        552     939     189    3640

  Fragments arranged by size: 

                3640     939     552     189
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5320
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5320
 
NotI GC'GGCC_GC

Cuts at:   2767    2767 
   Size:       5320
 
NspI r_CATG'y

Cuts at:   1132    1229    2126    2678    4223    1132 
   Size:         97     897     552    1545    2229

  Fragments arranged by size: 

                2229    1545     897     552      97
 
PasI CC'CwG_GG

Cuts at:   1348    2350    1348 
   Size:       1002    4318
 
PciI A'CATG_T

Cuts at:   2122    4219    2122 
   Size:       2097    3223
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5320
 
PmeI GTTT'AAAC

Cuts at:   2624    2624 
   Size:       5320
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3389    3421    4902    4934    3389 
   Size:         32    1481      32    3775

  Fragments arranged by size: 

                3775    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5320
 
PsiI TTA'TAA

Cuts at:   2890    2890 
   Size:       5320
 
PspOMI G'GGCC_C

Cuts at:   2019    2019 
   Size:       5320
 
PstI C_TGCA'G

Cuts at:    830    2045    2672     830 
   Size:       1215     627    3478

  Fragments arranged by size: 

                3478    1215     627
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5320
 
PvuII CAG'CTG

Cuts at:   2131    2239    2131 
   Size:        108    5212
 
SalI G'TCGA_C

Cuts at:   1936    2662    1936 
   Size:        726    4594
 
SbfI CC_TGCA'GG

Cuts at:   2672    2672 
   Size:       5320
 
ScaI AGT'ACT

Cuts at:   3568    5280    3568 
   Size:       1712    3608
 
SexAI A'CCwGG_T

Cuts at:   2214    2214 
   Size:       5320
 
SfcI C'TryA_G

Cuts at:    826    1045    2041    2668    3803    4484    4675     826 
   Size:        219     996     627    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135     996     681     627     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5320
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5320
 
SmaI CCC'GGG

Cuts at:   1872    2647    1872 
   Size:        775    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2425    3424    4325    4587     820 
   Size:        197     926     482     999     901     262    1553

  Fragments arranged by size: 

                1553     999     926     901     482     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3111     493 
   Size:       2618    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5320
 
SphI G_CATG'C

Cuts at:   1132    2678    1132 
   Size:       1546    3774
 
SspI AAT'ATT

Cuts at:      5      52    3244       5 
   Size:         47    3192    2081

  Fragments arranged by size: 

                3192    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5320
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2193    2256    2759     513 
   Size:        552     939     189      63     503    3074

  Fragments arranged by size: 

                3074     939     552     503     189      63
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3347    3364    3517    3702    4147     325 
   Size:       1255    1767      17     153     185     445    1498

  Fragments arranged by size: 

                1767    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2202    3566    5278 
   Size:        274      80      33      51    1443     225    1364    1712

Cuts at:   5278      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1364     274     225     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2449    2481    2550    2582    5280    5312    2449 
   Size:         32      69      32    2698      32    2457

  Fragments arranged by size: 

                2698    2457      69      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2264    1316 
   Size:        948    4372
 
XbaI T'CTAG_A

Cuts at:   2656    2656 
   Size:       5320
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5320
 
XmaI C'CCGG_G

Cuts at:   1870    2645    1870 
   Size:        775    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3449    3449 
   Size:       5320
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3128     276 
   Size:         53      83     186     936    1594    2468

  Fragments arranged by size: 

                2468    1594     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
   PspOMI     PstI     PvuI    PvuII     SalI     SbfI     ScaI    SexAI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I  EcoICRI    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PfoI     PmlI    PpuMI    PspXI     PsrI    RsrII     SacI
    SacII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI     XcmI
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