(Circular) (Six-base) MAPSORT of: pFN21AE4480.seq Check: 5762 from: 1 to: 6196 pFN21AE4480 6196 bp With 182 enzymes: SgfI * March 28, 2012 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4006 278 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2823 3539 1937 Size: 886 716 4594 Fragments arranged by size: 4594 886 716 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 2590 4323 4696 1563 Size: 1027 1733 373 3063 Fragments arranged by size: 3063 1733 1027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 3247 4259 5643 5911 1632 Size: 740 875 1012 1384 268 1917 Fragments arranged by size: 1917 1384 1012 875 740 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5999 AflIII A'CryG_T Cuts at: 1203 5095 6072 1203 Size: 3892 977 1327 Fragments arranged by size: 3892 1327 977 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 4925 4925 Size: 6196 AleI CACnn'nnGTG Cuts at: 1166 2768 1166 Size: 1602 4594 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3160 3192 3160 Size: 32 6164 AlwNI CAG_nnn'CTG Cuts at: 3939 5511 3939 Size: 1572 4624 ApaLI G'TGCA_C Cuts at: 2941 4253 5409 2941 Size: 1312 1156 3728 Fragments arranged by size: 3728 1312 1156 ApoI r'AATT_y Cuts at: 1493 3505 3731 3883 5992 1493 Size: 2012 226 152 2109 1697 Fragments arranged by size: 2109 2012 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 2151 3521 1870 Size: 73 208 1370 4545 Fragments arranged by size: 4545 1370 208 73 BamHI G'GATC_C Cuts at: 2279 2395 3526 3893 2279 Size: 116 1131 367 4582 Fragments arranged by size: 4582 1131 367 116 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3517 4972 618 Size: 325 217 627 1730 1455 1842 Fragments arranged by size: 1842 1730 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3515 1391 Size: 2124 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2073 928 Size: 971 174 5051 Fragments arranged by size: 5051 971 174 BbvCI CC'TCA_GC Cuts at: 2924 2924 Size: 6196 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2575 4088 5298 2575 Size: 1513 1210 3473 Fragments arranged by size: 3473 1513 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4807 136 Size: 107 122 71 631 605 3135 1525 Fragments arranged by size: 3135 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3251 6191 3251 Size: 2940 3256 BlpI GC'TnA_GC Cuts at: 2958 3114 3613 2958 Size: 156 499 5541 Fragments arranged by size: 5541 499 156 Bme1580I G_kGCm'C Cuts at: 1790 2904 2945 4257 5413 1790 Size: 1114 41 1312 1156 2573 Fragments arranged by size: 2573 1312 1156 1114 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6196 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2355 3927 4885 Size: 416 53 83 399 24 938 1572 958 Cuts at: 4885 442 Size: 1753 Fragments arranged by size: 1753 1572 958 938 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 4856 1239 Size: 1388 2229 2579 Fragments arranged by size: 2579 2229 1388 Bpu10I CC'TnA_GC Cuts at: 2924 3108 2924 Size: 184 6012 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4321 5186 5484 4321 Size: 865 298 5033 Fragments arranged by size: 5033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3203 4859 882 Size: 617 1704 1656 2219 Fragments arranged by size: 2219 1704 1656 617 BsaAI yAC'GTr Cuts at: 493 1204 3987 493 Size: 711 2783 2702 Fragments arranged by size: 2783 2702 711 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4003 4385 5020 275 Size: 53 83 186 936 2470 382 635 1451 Fragments arranged by size: 2470 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4629 5027 5301 5448 5859 4629 Size: 398 274 147 411 4966 Fragments arranged by size: 4966 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2649 2679 3160 3190 1178 Size: 30 165 30 1246 30 481 30 4184 Fragments arranged by size: 4184 1246 481 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2806 1186 Size: 1294 326 4576 Fragments arranged by size: 4576 1294 326 BseYI C'CCAG_C Cuts at: 5399 5895 5399 Size: 496 5700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5623 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3646 4407 5435 1540 Size: 275 186 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2945 4257 4342 5413 2945 Size: 1312 85 1071 3728 Fragments arranged by size: 3728 1312 1071 85 BsmI GAATG_Cn' Cuts at: 2235 3707 3800 2235 Size: 1472 93 4631 Fragments arranged by size: 4631 1472 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2904 2945 3515 4257 4342 5413 1391 Size: 399 1114 41 570 742 85 1071 2174 Fragments arranged by size: 2174 1114 1071 742 570 399 85 41 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 2464 4085 5815 2464 Size: 1621 1730 2845 Fragments arranged by size: 2845 1730 1621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2167 2788 3190 3551 844 Size: 1323 621 402 361 3489 Fragments arranged by size: 3489 1323 621 402 361 BsrBI CCG'CTC Cuts at: 1394 1823 4083 1394 Size: 429 2260 3507 Fragments arranged by size: 3507 2260 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4691 4865 65 Size: 1183 3443 174 1396 Fragments arranged by size: 3443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4840 5027 1700 Size: 110 3030 187 2869 Fragments arranged by size: 3030 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2406 2732 96 Size: 2310 326 3560 Fragments arranged by size: 3560 2310 326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6196 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6196 BstYI r'GATC_y Cuts at: 1972 2279 2395 2659 3251 3526 3893 4278 4295 Size: 307 116 264 592 275 367 385 17 Cuts at: 4295 5747 5833 5845 6191 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 592 385 367 346 307 275 264 116 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2789 2789 Size: 6196 BtgI C'CryG_G Cuts at: 513 1065 2577 513 Size: 552 1512 4132 Fragments arranged by size: 4132 1512 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 3712 4506 4526 3712 Size: 794 20 5382 Fragments arranged by size: 5382 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6161 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3643 4532 6161 Size: 54 1425 50 275 18 1813 889 1629 Cuts at: 6161 8 Size: 43 Fragments arranged by size: 1813 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3643 1537 Size: 275 1831 4090 Fragments arranged by size: 4090 1831 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4126 1353 Size: 2773 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4783 5157 5303 387 Size: 1064 138 3194 374 146 1280 Fragments arranged by size: 3194 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2627 3247 4259 4856 5643 5911 Size: 393 740 255 620 1012 597 787 268 Cuts at: 5911 1239 Size: 1524 Fragments arranged by size: 1524 1012 787 740 620 597 393 268 255 EcoO109I rG'GnC_Cy Cuts at: 2143 2143 Size: 6196 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 EcoRV GAT'ATC Cuts at: 2701 2701 Size: 6196 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 5343 1678 Size: 3665 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2301 2333 2380 2412 3236 Size: 32 135 32 729 32 47 32 824 Cuts at: 3236 3268 4837 4869 4911 4943 1373 Size: 32 1569 32 42 32 2626 Fragments arranged by size: 2626 1569 824 729 135 47 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2257 2314 3337 3540 3786 669 Size: 988 281 319 57 1023 203 246 3079 Fragments arranged by size: 3079 1023 988 319 281 246 203 57 HindIII A'AGCT_T Cuts at: 748 3277 748 Size: 2529 3667 HpaI GTT'AAC Cuts at: 2314 3337 3786 2314 Size: 1023 449 4724 Fragments arranged by size: 4724 1023 449 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2423 3253 3428 5310 5494 1305 Size: 1118 830 175 1882 184 2007 Fragments arranged by size: 2007 1882 1118 830 184 175 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4374 Fragments arranged by size: 4374 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2768 4154 4513 4672 518 Size: 648 1602 1386 359 159 2042 Fragments arranged by size: 2042 1602 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2957 3612 4289 5437 5682 1606 Size: 1351 655 677 1148 245 2120 Fragments arranged by size: 2120 1351 1148 677 655 245 NcoI C'CATG_G Cuts at: 513 1065 2577 513 Size: 552 1512 4132 Fragments arranged by size: 4132 1512 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6196 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2769 3554 5099 1132 Size: 97 1540 785 1545 2229 Fragments arranged by size: 2229 1545 1540 785 97 PasI CC'CwG_GG Cuts at: 1348 2905 1348 Size: 1557 4639 PciI A'CATG_T Cuts at: 5095 5095 Size: 6196 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3160 3192 4265 4297 5778 5810 3160 Size: 32 1073 32 1481 32 3546 Fragments arranged by size: 3546 1481 1073 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2143 Size: 6196 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 3548 830 Size: 2718 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 2305 3544 4679 5360 5551 826 Size: 219 1260 1239 1135 681 191 1471 Fragments arranged by size: 1471 1260 1239 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 3523 1872 Size: 1651 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4300 5201 5463 820 Size: 197 926 2357 901 262 1553 Fragments arranged by size: 2357 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3554 1132 Size: 2422 3774 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2577 3635 513 Size: 552 1512 1058 3074 Fragments arranged by size: 3074 1512 1058 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2041 4223 4240 4393 4578 5023 325 Size: 1255 461 2182 17 153 185 445 1498 Fragments arranged by size: 2182 1498 1255 461 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2406 2732 3230 Size: 274 80 33 51 1443 429 326 498 Cuts at: 3230 4442 6154 96 Size: 1212 1712 138 Fragments arranged by size: 1712 1443 1212 498 429 326 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2649 2681 6156 6188 2649 Size: 32 3475 32 2657 Fragments arranged by size: 3475 2657 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6196 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2902 2902 Size: 6196 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 3521 1870 Size: 1651 4545 XmnI GAAnn'nnTTC Cuts at: 4325 4325 Size: 6196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4004 276 Size: 53 83 186 936 2470 2468 Fragments arranged by size: 2470 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI