################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflIII AgeI AhdI AlwI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbsI BbvCI BciVI BfaI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DsaI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 610 GACGTC GACGT^C AcyI 610 GACGTC G[GA]^CG[CT]C AflII 286 CTTAAG C^TTAAG Alw44I 563 GTGCAC G^TGCAC Aor51HI 293 AGCGCT AGC^GCT ApaLI 563 GTGCAC G^TGCAC BalI 690 TGGCCA TGG^CCA BanI 678 GGTGCC G^G[CT][GA]CC BbrPI 865 CACGTG CAC^GTG BcgI 491 CGAGCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 525 TGATCA T^GATCA BfrI 286 CTTAAG C^TTAAG BsaAI 865 CACGTG [CT]AC^GT[GA] BsaHI 610 GACGTC G[GA]^CG[CT]C BsaWI 17 ACCGGA [AT]^CCGG[AT] BsgI 673 GTGCAG GTGCAG(16/14) BsiEI 41 CGGCCG CG[GA][CT]^CG BsmAI 107 GAGAC GTCTC(1/5) BsmFI 452 GGGAC GGGAC(10/14) BspMI 675 GCAGGT ACCTGC(4/8) BsrBI 24 CCGCTC CCGCTC(-3/-3) BsrDI 486 CATTGC GCAATG(2/0) BssHII 82 GCGCGC G^CGCGC BssSI 310 CACGAG CACGAG(-5/-1) BstEII 654 GGTGACC G^GT[ACGT]ACC BstPI 654 GGTGACC G^GT[ACGT]ACC BstXI 215 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 550 CTCAG C^T[ACGT]AG DpnII 526 GATC ^GATC DraII 509 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 649 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 325 GACAAGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 41 CGGCCG C^GGCCG EarI 229 GAAGAG CTCTTC(1/4) EclXI 41 CGGCCG C^GGCCG Eco47III 293 AGCGCT AGC^GCT Eco52I 41 CGGCCG C^GGCCG EcoO109I 509 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 654 GGTGACC G^GT[ACGT]ACC FbaI 525 TGATCA T^GATCA HgaI 803 GCGTC GACGC(5/10) Hin1I 610 GACGTC G[GA]^CG[CT]C HphI 654 GGTGA GGTGA(8/7) Ksp632I 229 GAAGAG CTCTTC(1/4) MboI 526 GATC ^GATC MluNI 690 TGGCCA TGG^CCA MscI 690 TGGCCA TGG^CCA MseI 287 TTAA T^TAA NdeII 526 GATC ^GATC PmaCI 865 CACGTG CAC^GTG PmlI 865 CACGTG CAC^GTG PvuII 94 CAGCTG CAG^CTG Sau3AI 526 GATC ^GATC Tru9I 287 TTAA T^TAA XcmI 215 CCATTATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AluI 95 AGCT AG^CT 764 AGCT BanII 49 GGGCTC G[GA]GC[CT]^C 313 GAGCCC BcnI 54 CCCGG CC^[GC]GG 682 CCGGG BglI 161 GCCAGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 692 GCCAGGATGGC BsrFI 302 ACCGGC [GA]^CCGG[CT] 467 ACCGGC Cfr10I 302 ACCGGC [GA]^CCGG[CT] 467 ACCGGC EaeI 41 CGGCCG [CT]^GGCC[GA] 690 TGGCCA EcoT14I 414 CCTTGG C^C[AT][AT]GG 605 CCAAGG HaeII 293 AGCGCT [GA]GCGC^[CT] 474 GGCGCT HinfI 211 GACTC G^A[ACGT]TC 357 GACTC MslI 201 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 861 CATCCACGTG MspA1I 94 CAGCTG C[AC]G^C[GT]G 175 CAGCGG NciI 54 CCCGG CC^[GC]GG 682 CCGGG PleI 211 GACTC GAGTC(4/5) 357 GACTC StyI 414 CCTTGG C^C[AT][AT]GG 605 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AfaI AspHI AvaII BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboII MnlI MspI MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SmlI TaqI TseI Tsp45I TspRI TthHB8I