Restriction map for
pFN21AE1438
(Linear) (Six-base) MAPSORT of: KIEE1438.seq Check: 2336 from: 1 to: 885
KIEE1438.orf
With 182 enzymes: SgfI *
July 15, 2010 18:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 666 885
Size: 666 219
AatII G_ACGT'C
Cuts at: 0 614 885
Size: 614 271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 295 885
Size: 222 73 590
Fragments arranged by size:
590 222 73
AfeI AGC'GCT
Cuts at: 0 295 885
Size: 295 590
AflII C'TTAA_G
Cuts at: 0 286 885
Size: 286 599
AleI CACnn'nnGTG
Cuts at: 0 205 885
Size: 205 680
ApaLI G'TGCA_C
Cuts at: 0 563 885
Size: 563 322
BanI G'GyrC_C
Cuts at: 0 678 885
Size: 678 207
BanII G_rGCy'C
Cuts at: 0 53 317 885
Size: 53 264 568
Fragments arranged by size:
568 264 53
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 480 514 885
Size: 480 34 371
Fragments arranged by size:
480 371 34
BclI T'GATC_A
Cuts at: 0 525 885
Size: 525 360
BglI GCCn_nnn'nGGC
Cuts at: 0 167 698 885
Size: 167 531 187
Fragments arranged by size:
531 187 167
Bme1580I G_kGCm'C
Cuts at: 0 377 567 885
Size: 377 190 318
Fragments arranged by size:
377 318 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 212 372 885
Size: 212 160 513
Fragments arranged by size:
513 212 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 856 885
Size: 732 124 29
Fragments arranged by size:
732 124 29
BsaAI yAC'GTr
Cuts at: 0 867 885
Size: 867 18
BsaHI Gr'CG_yC
Cuts at: 0 611 885
Size: 611 274
BsaWI w'CCGG_w
Cuts at: 0 17 885
Size: 17 868
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 885
Size: 694 191
BsiEI CG_ry'CG
Cuts at: 0 44 885
Size: 44 841
BsiHKAI G_wGCw'C
Cuts at: 0 185 467 567 872 885
Size: 185 282 100 305 13
Fragments arranged by size:
305 282 185 100 13
Bsp1286I G_dGCh'C
Cuts at: 0 53 185 317 377 467 567 872 885
Size: 53 132 132 60 90 100 305 13
Fragments arranged by size:
305 132 132 100 90 60 53 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 885
Size: 666 219
BsrBI CCG'CTC
Cuts at: 0 26 885
Size: 26 859
BsrDI GCAATG_nn'
Cuts at: 0 485 885
Size: 485 400
BsrFI r'CCGG_y
Cuts at: 0 302 467 885
Size: 302 165 418
Fragments arranged by size:
418 302 165
BssHII G'CGCG_C
Cuts at: 0 82 885
Size: 82 803
BssSI C'ACGA_G
Cuts at: 0 310 885
Size: 310 575
BstEII G'GTnAC_C
Cuts at: 0 654 885
Size: 654 231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 885
Size: 222 663
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 514 885
Size: 514 371
DraIII CAC_nnn'GTG
Cuts at: 0 654 885
Size: 654 231
DrdI GACnn_nn'nnGTC
Cuts at: 0 331 885
Size: 331 554
EaeI y'GGCC_r
Cuts at: 0 41 690 885
Size: 41 649 195
Fragments arranged by size:
649 195 41
EagI C'GGCC_G
Cuts at: 0 41 885
Size: 41 844
EarI CTCTTCn'nnn_
Cuts at: 0 224 885
Size: 224 661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 295 885
Size: 222 73 590
Fragments arranged by size:
590 222 73
EcoO109I rG'GnC_Cy
Cuts at: 0 510 885
Size: 510 375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 615 885
Size: 615 270
HaeII r_GCGC'y
Cuts at: 0 297 478 885
Size: 297 181 407
Fragments arranged by size:
407 297 181
MscI TGG'CCA
Cuts at: 0 692 885
Size: 692 193
MslI CAynn'nnrTG
Cuts at: 0 205 865 885
Size: 205 660 20
Fragments arranged by size:
660 205 20
MspA1I CmG'CkG
Cuts at: 0 96 177 885
Size: 96 81 708
Fragments arranged by size:
708 96 81
PasI CC'CwG_GG
Cuts at: 0 30 571 885
Size: 30 541 314
Fragments arranged by size:
541 314 30
PfoI T'CCnGG_A
Cuts at: 0 53 885
Size: 53 832
PmlI CAC'GTG
Cuts at: 0 867 885
Size: 867 18
PvuII CAG'CTG
Cuts at: 0 96 885
Size: 96 789
SmlI C'TyrA_G
Cuts at: 0 286 711 871 885
Size: 286 425 160 14
Fragments arranged by size:
425 286 160 14
StyI C'CwwG_G
Cuts at: 0 414 605 885
Size: 414 191 280
Fragments arranged by size:
414 280 191
TatI w'GTAC_w
Cuts at: 0 131 553 885
Size: 131 422 332
Fragments arranged by size:
422 332 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 885
Size: 222 663
ZraI GAC'GTC
Cuts at: 0 612 885
Size: 612 273
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AleI ApaLI BanI
BanII BcgI BclI BglI Bme1580I BmrI BpuEI BsaAI
BsaHI BsaWI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstEII BstXI BtgZI DraIII
DrdI EaeI EagI EarI Eco57MI EcoO109I FalI HaeII
MscI MslI MspA1I PasI PfoI PmlI PvuII SmlI
StyI TatI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflIII AgeI AhdI AlfI AloI
AlwNI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI BglII
BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI BsaBI
BsaXI BseRI BseYI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI