(Circular) (Six-base) MAPSORT of: pFN21AB0487.seq Check: 8063 from: 1 to: 6292 pFN21AB0487 6292 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2478 3635 1937 Size: 541 1157 4594 Fragments arranged by size: 4594 1157 541 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 4355 5739 6007 1632 Size: 620 2103 1384 268 1917 Fragments arranged by size: 2103 1917 1384 620 268 AfeI AGC'GCT Cuts at: 2467 2467 Size: 6292 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 2783 3270 1166 Size: 1617 487 4188 Fragments arranged by size: 4188 1617 487 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2783 1184 Size: 1599 4693 AlwNI CAG_nnn'CTG Cuts at: 2273 4035 5607 2273 Size: 1762 1572 2958 Fragments arranged by size: 2958 1762 1572 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 2096 2150 2358 2923 3601 3827 3979 6088 Size: 603 54 208 565 678 226 152 2109 Cuts at: 6088 1493 Size: 1697 Fragments arranged by size: 2109 1697 678 603 565 226 208 152 54 AseI AT'TA_AT Cuts at: 160 1032 2381 4846 160 Size: 872 1349 2465 1606 Fragments arranged by size: 2465 1606 1349 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3617 1870 Size: 73 1674 4545 Fragments arranged by size: 4545 1674 73 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3613 5068 618 Size: 325 217 627 1826 1455 1842 Fragments arranged by size: 1842 1826 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2989 3611 1391 Size: 1598 622 4072 Fragments arranged by size: 4072 1598 622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3207 3287 928 Size: 971 1308 80 3933 Fragments arranged by size: 3933 1308 971 80 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2152 2333 2998 4184 5394 2152 Size: 181 665 1186 1210 3050 Fragments arranged by size: 3050 1210 1186 665 181 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 4353 5509 1790 Size: 2563 1156 2573 Fragments arranged by size: 2573 2563 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2091 4952 1239 Size: 852 2861 2579 Fragments arranged by size: 2861 2579 852 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4417 5282 5580 4417 Size: 865 298 5129 Fragments arranged by size: 5129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3026 4955 882 Size: 617 1527 1929 2219 Fragments arranged by size: 2219 1929 1527 617 BsaAI yAC'GTr Cuts at: 493 1204 2402 4083 493 Size: 711 1198 1681 2702 Fragments arranged by size: 2702 1681 1198 711 BsaBI GATnn'nnATC Cuts at: 2792 6286 2792 Size: 3494 2798 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4099 4481 5116 275 Size: 53 83 186 936 2566 382 635 1451 Fragments arranged by size: 2566 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6067 Fragments arranged by size: 6067 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6292 BseYI C'CCAG_C Cuts at: 2811 5495 5991 2811 Size: 2684 496 3112 Fragments arranged by size: 3112 2684 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5719 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2893 4353 4438 5509 2893 Size: 1460 85 1071 3676 Fragments arranged by size: 3676 1460 1071 85 BsmI GAATG_Cn' Cuts at: 2180 3090 3803 3896 2180 Size: 910 713 93 4576 Fragments arranged by size: 4576 910 713 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2893 2989 3611 4353 4438 5509 1391 Size: 399 1103 96 622 742 85 1071 2174 Fragments arranged by size: 2174 1103 1071 742 622 399 96 85 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 4181 5911 4181 Size: 1730 4562 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2900 3647 844 Size: 2056 747 3489 Fragments arranged by size: 3489 2056 747 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2847 3156 4787 4961 65 Size: 1183 1599 309 1631 174 1396 Fragments arranged by size: 1631 1599 1396 1183 309 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4936 5123 1700 Size: 110 3126 187 2869 Fragments arranged by size: 3126 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 3041 4352 5364 3041 Size: 1311 1012 3969 Fragments arranged by size: 3969 1311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstYI r'GATC_y Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287 Size: 1650 367 385 17 1452 86 12 346 Cuts at: 6287 1972 Size: 1977 Fragments arranged by size: 1977 1650 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5740 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2563 487 Size: 683 56 1337 4216 Fragments arranged by size: 4216 1337 683 56 BtsI GCAGTG_nn' Cuts at: 3808 4602 4622 3808 Size: 794 20 5478 Fragments arranged by size: 5478 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 2922 3596 2922 Size: 674 5618 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2844 2928 3739 Size: 54 1425 50 275 18 1014 84 811 Cuts at: 3739 4628 6257 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1014 889 811 275 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3408 4222 1353 Size: 2055 814 3423 Fragments arranged by size: 3423 2055 814 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2091 2252 4355 4952 5739 6007 1239 Size: 393 459 161 2103 597 787 268 1524 Fragments arranged by size: 2103 1524 787 597 459 393 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 2583 2583 Size: 6292 EcoRI G'AATT_C Cuts at: 2358 3601 3979 2358 Size: 1243 378 4671 Fragments arranged by size: 4671 1243 378 EcoRV GAT'ATC Cuts at: 2724 2724 Size: 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3521 3521 Size: 6292 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 2469 5439 1678 Size: 791 2970 2531 Fragments arranged by size: 2970 2531 791 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3274 3306 4933 4965 5007 Size: 32 135 32 1702 32 1627 32 42 Cuts at: 5007 5039 1373 Size: 32 2626 Fragments arranged by size: 2626 1702 1627 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 2666 748 Size: 1918 4374 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3537 3891 3537 Size: 354 5938 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2866 3338 5406 5590 1305 Size: 1561 472 2068 184 2007 Fragments arranged by size: 2068 2007 1561 472 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2846 2930 10 Size: 54 1425 343 1014 84 3372 Fragments arranged by size: 3372 1425 1014 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2783 3270 4250 4609 4768 518 Size: 648 843 774 487 980 359 159 2042 Fragments arranged by size: 2042 980 843 774 648 487 359 159 MspA1I CmG'CkG Cuts at: 1606 2218 2263 2714 3708 4385 5533 5778 1606 Size: 612 45 451 994 677 1148 245 2120 Fragments arranged by size: 2120 1148 994 677 612 451 245 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5740 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 2939 3650 5195 1132 Size: 97 1710 711 1545 2229 Fragments arranged by size: 2229 1710 1545 711 97 PasI CC'CwG_GG Cuts at: 1348 2387 1348 Size: 1039 5253 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6292 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PmlI CAC'GTG Cuts at: 2402 2402 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2642 2674 4361 4393 5874 5906 2642 Size: 32 1687 32 1481 32 3028 Fragments arranged by size: 3028 1687 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PstI C_TGCA'G Cuts at: 830 2719 3644 830 Size: 1889 925 3478 Fragments arranged by size: 3478 1889 925 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 2218 2714 2218 Size: 496 5796 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SfcI C'TryA_G Cuts at: 826 1045 2715 3640 4775 5456 5647 826 Size: 219 1670 925 1135 681 191 1471 Fragments arranged by size: 1670 1471 1135 925 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4396 5297 5559 820 Size: 197 926 2453 901 262 1553 Fragments arranged by size: 2453 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 2939 3650 1132 Size: 1807 711 3774 Fragments arranged by size: 3774 1807 711 SspI AAT'ATT Cuts at: 5 52 2764 4216 5 Size: 47 2712 1452 2081 Fragments arranged by size: 2712 2081 1452 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 3731 513 Size: 552 2666 3074 Fragments arranged by size: 3074 2666 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 2869 4319 4336 4489 4674 5119 Size: 1255 1215 74 1450 17 153 185 445 Cuts at: 5119 325 Size: 1498 Fragments arranged by size: 1498 1450 1255 1215 445 185 153 74 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4538 6250 96 Size: 274 80 33 51 1443 2561 1712 138 Fragments arranged by size: 2561 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6252 6284 6252 Size: 32 6260 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 2996 3628 2996 Size: 632 5660 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3103 3103 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 2362 3076 4421 2362 Size: 714 1345 4233 Fragments arranged by size: 4233 1345 714 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI