(Circular) (Six-base) MAPSORT of: pFN21AB7603.seq Check: 1685 from: 1 to: 5596 pFN21AB7603 5596 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2211 2696 2917 2211 Size: 485 221 4890 Fragments arranged by size: 4890 485 221 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2559 3659 5043 5311 1632 Size: 927 1100 1384 268 1917 Fragments arranged by size: 1917 1384 1100 927 268 AflII C'TTAA_G Cuts at: 820 1017 2018 820 Size: 197 1001 4398 Fragments arranged by size: 4398 1001 197 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AlwNI CAG_nnn'CTG Cuts at: 3339 4911 3339 Size: 1572 4024 ApaI G_GGCC'C Cuts at: 2106 2106 Size: 5596 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2882 2905 3131 3283 5392 1493 Size: 1389 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1389 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2166 2261 2382 2921 1870 Size: 73 155 68 95 121 539 4545 Fragments arranged by size: 4545 539 155 121 95 73 68 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 2696 2917 4372 618 Size: 325 217 627 424 485 221 1455 1842 Fragments arranged by size: 1842 1455 627 485 424 325 221 217 BanII G_rGCy'C Cuts at: 1391 2106 2489 2915 1391 Size: 715 383 426 4072 Fragments arranged by size: 4072 715 426 383 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2375 928 Size: 971 476 4149 Fragments arranged by size: 4149 971 476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BfrBI ATG'CAT Cuts at: 2653 2653 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5591 5591 Size: 5596 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 2106 3657 4813 1790 Size: 316 1551 1156 2573 Fragments arranged by size: 2573 1551 1156 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2361 2503 4256 1239 Size: 1122 142 1753 2579 Fragments arranged by size: 2579 1753 1122 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2347 4259 882 Size: 617 848 1912 2219 Fragments arranged by size: 2219 1912 848 617 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2518 1186 Size: 1063 269 4264 Fragments arranged by size: 4264 1063 269 BseYI C'CCAG_C Cuts at: 2488 2731 4799 5295 2488 Size: 243 2068 496 2789 Fragments arranged by size: 2789 2068 496 243 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2294 1163 Size: 573 327 231 4465 Fragments arranged by size: 4465 573 327 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2470 3657 3742 4813 2470 Size: 1187 85 1071 3253 Fragments arranged by size: 3253 1187 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2470 2489 2915 3657 3742 4813 Size: 399 316 364 19 426 742 85 1071 Cuts at: 4813 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 426 399 364 316 85 19 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2266 2365 2951 844 Size: 1422 99 586 3489 Fragments arranged by size: 3489 1422 586 99 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2451 4091 4265 65 Size: 1183 1203 1640 174 1396 Fragments arranged by size: 1640 1396 1203 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 2875 3656 4668 2875 Size: 781 1012 3803 Fragments arranged by size: 3803 1012 781 BstAPI GCAn_nnn'nTGC Cuts at: 794 2198 794 Size: 1404 4192 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2756 2756 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2512 2926 3293 3678 3695 5147 5233 5245 Size: 540 414 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 540 414 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2088 2691 513 Size: 552 1023 603 3418 Fragments arranged by size: 3418 1023 603 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2470 3112 3906 3926 2470 Size: 642 794 20 4140 Fragments arranged by size: 4140 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2413 3043 3932 Size: 54 1425 50 275 18 583 630 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 630 583 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2290 2536 3526 1353 Size: 937 246 990 3423 Fragments arranged by size: 3423 990 937 246 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2361 2503 2559 3659 4256 5043 5311 Size: 393 729 142 56 1100 597 787 268 Cuts at: 5311 1239 Size: 1524 Fragments arranged by size: 1524 1100 787 729 597 393 268 142 56 EcoO109I rG'GnC_Cy Cuts at: 2102 2103 2547 2102 Size: 1 444 5151 Fragments arranged by size: 5151 444 1 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2851 2851 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 2146 4743 1678 Size: 468 2597 2531 Fragments arranged by size: 2597 2531 468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2149 2181 2504 Size: 32 135 32 427 32 118 32 323 Cuts at: 2504 2536 2551 2568 2583 2618 2650 2855 2887 Size: 32 15 17 15 35 32 205 32 Cuts at: 2887 4237 4269 4311 4343 1373 Size: 1350 32 42 32 2626 Fragments arranged by size: 2626 1350 427 323 205 135 118 42 35 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 2767 2828 748 Size: 2019 61 3516 Fragments arranged by size: 3516 2019 61 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2215 2700 2921 2215 Size: 485 221 4890 Fragments arranged by size: 4890 485 221 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2415 10 Size: 54 1425 343 583 3191 Fragments arranged by size: 3191 1425 583 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2875 3554 3913 4072 518 Size: 648 1059 650 679 359 159 2042 Fragments arranged by size: 2042 1059 679 650 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3012 3689 4837 5082 1606 Size: 1406 677 1148 245 2120 Fragments arranged by size: 2120 1406 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2691 513 Size: 552 1626 3418 Fragments arranged by size: 3418 1626 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NsiI A_TGCA'T Cuts at: 2655 2655 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2542 2954 4499 1132 Size: 97 1313 412 1545 2229 Fragments arranged by size: 2229 1545 1313 412 97 PasI CC'CwG_GG Cuts at: 1348 2106 2749 1348 Size: 758 643 4195 Fragments arranged by size: 4195 758 643 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2893 1108 Size: 1785 3811 PfoI T'CCnGG_A Cuts at: 2665 2822 2665 Size: 157 5439 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2547 2547 Size: 5596 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 2805 2859 3166 2805 Size: 54 307 5235 Fragments arranged by size: 5235 307 54 PspOMI G'GGCC_C Cuts at: 2102 2102 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SapI GCTCTTCn'nnn_ Cuts at: 2290 2290 Size: 5596 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2944 4079 4760 4951 826 Size: 219 1899 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2100 2923 1872 Size: 228 823 4545 Fragments arranged by size: 4545 823 228 SmlI C'TyrA_G Cuts at: 820 1017 1943 2018 3700 4601 4863 820 Size: 197 926 75 1682 901 262 1553 Fragments arranged by size: 1682 1553 926 901 262 197 75 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2542 2954 1132 Size: 1410 412 3774 Fragments arranged by size: 3774 1410 412 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2691 3035 513 Size: 552 1626 344 3074 Fragments arranged by size: 3074 1626 552 344 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2762 3623 3640 3793 3978 4423 325 Size: 1255 1182 861 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1182 861 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2671 2703 5556 5588 2671 Size: 32 2853 32 2679 Fragments arranged by size: 2853 2679 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2794 2065 Size: 729 4867 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2098 2921 1870 Size: 228 823 4545 Fragments arranged by size: 4545 823 228 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI