Restriction map for
pFN21AE1042
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbeI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI Eco47III Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NarI NcoI NdeI
NotI NruI NsiI NspI NspV
PacI PflMI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 1443 GAATTC [GA]^AATT[CT]
AgeI 152 ACCGGT A^CCGGT
AhdI 399 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 588 GATCC GGATC(4/5)
ApaI 1245 GGGCCC GGGCC^C
ApoI 1443 GAATTC [GA]^AATT[CT]
AspEI 399 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 610 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1324 CACGTG CAC^GTG
BbvCI 183 CCTCAGC CCTCAGC(-5/-2)
BcgI 637 CGAGTCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 11 CTAG C^TAG
BglI 371 GCCACGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1070 AGATCT A^GATCT
BsaI 776 GAGACC GGTCTC(1/5)
BsaWI 152 ACCGGT [AT]^CCGG[AT]
BseRI 975 GAGGAG GAGGAG(10/8)
BsiEI 311 CGGCCG CG[GA][CT]^CG
BspMI 1151 GCAGGT ACCTGC(4/8)
BsrBI 388 CCGCTC CCGCTC(-3/-3)
BsrDI 493 CATTGC GCAATG(2/0)
BstEII 1287 GGTGACC G^GT[ACGT]ACC
BstPI 1287 GGTGACC G^GT[ACGT]ACC
DraIII 703 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 311 CGGCCG C^GGCCG
Eam1105I 399 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 311 CGGCCG C^GGCCG
Eco52I 311 CGGCCG C^GGCCG
EcoO65I 1287 GGTGACC G^GT[ACGT]ACC
EcoRI 1443 GAATTC G^AATTC
EcoT14I 432 CCTTGG C^C[AT][AT]GG
HincII 1378 GTCAAC GT[CT]^[GA]AC
HindII 1378 GTCAAC GT[CT]^[GA]AC
MaeI 11 CTAG C^TAG
NheI 10 GCTAGC G^CTAGC
PaeR7I 670 CTCGAG C^TCGAG
PflFI 610 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 152 ACCGGT A^CCGGT
PleI 638 GAGTC GAGTC(4/5)
PmaCI 1324 CACGTG CAC^GTG
PmlI 1324 CACGTG CAC^GTG
PvuII 967 CAGCTG CAG^CTG
SmaI 593 CCCGGG CCC^GGG
StyI 432 CCTTGG C^C[AT][AT]GG
TfiI 650 GATTC G^A[AT]TC
Tsp509I 1444 AATT ^AATT
Tth111I 610 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 670 CTCGAG C^TCGAG
XmaCI 593 CCCGGG C^CCGGG
XmaI 593 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 411 CGCG CG^CG
789 CGCG
AcyI 1255 GACGCC G[GA]^CG[CT]C
1333 GACGCC
AlwNI 712 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
964 CAGCAGCTG
AvaI 593 CCCGGG C^[CT]CG[GA]G
670 CTCGAG
BanI 501 GGCACC G^G[CT][GA]CC
1198 GGCACC
BbsI 831 GAAGAC GAAGAC(2/6)
1057 GTCTTC
BpuAI 831 GAAGAC GAAGAC(2/6)
1057 GTCTTC
BsaAI 1324 CACGTG [CT]AC^GT[GA]
1450 TACGTG
BsaHI 1255 GACGCC G[GA]^CG[CT]C
1333 GACGCC
BsgI 709 GTGCAG GTGCAG(16/14)
961 GTGCAG
BsmBI 1422 CGTCTC CGTCTC(1/5)
673 GAGACG
BsoBI 593 CCCGGG C^[CT]CG[GA]G
670 CTCGAG
BsrI 323 CCAGT ACTGG(1/-1)
1375 CCAGT
BstAPI 702 GCACGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
711 GCAGTGGCTGC
BstUI 411 CGCG CG^CG
789 CGCG
BstYI 587 AGATCC [GA]^GATC[CT]
1070 AGATCT
DpnII 588 GATC ^GATC
1071 GATC
EarI 1225 CTCTTC CTCTTC(1/4)
1411 CTCTTC
HaeII 446 AGCGCC [GA]GCGC^[CT]
1091 AGCGCC
Hin1I 1255 GACGCC G[GA]^CG[CT]C
1333 GACGCC
HinfI 638 GAGTC G^A[ACGT]TC
650 GATTC
Ksp632I 1225 CTCTTC CTCTTC(1/4)
1411 CTCTTC
MboI 588 GATC ^GATC
1071 GATC
MflI 587 AGATCC [GA]^GATC[CT]
1070 AGATCT
MseI 559 TTAA T^TAA
1294 TTAA
MspA1I 381 CCGCTG C[AC]G^C[GT]G
967 CAGCTG
MvnI 411 CGCG CG^CG
789 CGCG
NdeII 588 GATC ^GATC
1071 GATC
PpuMI 346 GGGACCC [GA]G^G[AT]CC[CT]
992 AGGACCT
SacI 744 GAGCTC GAGCT^C
799 GAGCTC
Sau3AI 588 GATC ^GATC
1071 GATC
SmlI 670 CTCGAG C^T[CT][GA]AG
988 CTCAAG
StuI 420 AGGCCT AGG^CCT
902 AGGCCT
Tru9I 559 TTAA T^TAA
1294 TTAA
XcmI 59 CCAAGACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
135 CCAGGCGCAGTGTGG
XhoII 587 AGATCC [GA]^GATC[CT]
1070 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BalI BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BssKI
BssSI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboII MluNI MnlI MscI MspI
MvaI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PstI RsaI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I