(Circular) (Six-base) MAPSORT of: pFN21AE1042.seq Check: 7309 from: 1 to: 6208 pFN21AE1042 6208 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3328 4271 5655 5923 1632 Size: 1696 943 1384 268 1917 Fragments arranged by size: 1917 1696 1384 943 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 2157 5039 2157 Size: 2882 3326 AhdI GACnn_n'nnGTC Cuts at: 2409 4937 2409 Size: 2528 3680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6208 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2530 3424 1184 Size: 1346 894 3968 Fragments arranged by size: 3968 1346 894 AlwNI CAG_nnn'CTG Cuts at: 2722 2974 3951 5523 2722 Size: 252 977 1572 3407 Fragments arranged by size: 3407 1572 977 252 ApaI G_GGCC'C Cuts at: 3254 3254 Size: 6208 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 3448 3517 3743 3895 6004 1493 Size: 1955 69 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2598 2675 3533 1870 Size: 73 655 77 858 4545 Fragments arranged by size: 4545 858 655 77 73 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2506 3203 3529 4984 618 Size: 325 217 627 719 697 326 1455 1842 Fragments arranged by size: 1842 1455 719 697 627 326 325 217 BanII G_rGCy'C Cuts at: 1391 2753 2808 3254 3507 3527 1391 Size: 1362 55 446 253 20 4072 Fragments arranged by size: 4072 1362 446 253 55 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2843 3055 928 Size: 971 944 212 4081 Fragments arranged by size: 4081 971 944 212 BbvCI CC'TCA_GC Cuts at: 2189 2189 Size: 6208 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2631 2665 4399 4433 1903 Size: 34 694 34 1734 34 3678 Fragments arranged by size: 3678 1734 694 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2382 4819 136 Size: 107 122 71 631 605 710 2437 1525 Fragments arranged by size: 2437 1525 710 631 605 122 107 71 BglII A'GATC_T Cuts at: 3075 6203 3075 Size: 3128 3080 BlpI GC'TnA_GC Cuts at: 3625 3625 Size: 6208 Bme1580I G_kGCm'C Cuts at: 1790 2474 2509 3254 4269 5425 1790 Size: 684 35 745 1015 1156 2573 Fragments arranged by size: 2573 1156 1015 745 684 35 BmgBI CAC'GTC Cuts at: 1318 2527 2620 3355 1318 Size: 1209 93 735 4171 Fragments arranged by size: 4171 1209 735 93 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2322 3939 4897 Size: 416 53 83 399 24 905 1617 958 Cuts at: 4897 442 Size: 1753 Fragments arranged by size: 1753 1617 958 905 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2019 1056 Size: 963 5245 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2480 2780 2849 2996 4868 1239 Size: 821 420 300 69 147 1872 2579 Fragments arranged by size: 2579 1872 821 420 300 147 69 Bpu10I CC'TnA_GC Cuts at: 2189 2486 2189 Size: 297 5911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 4333 5198 5496 2978 Size: 1355 865 298 3690 Fragments arranged by size: 3690 1355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2775 4871 882 Size: 617 1276 2096 2219 Fragments arranged by size: 2219 2096 1276 617 BsaAI yAC'GTr Cuts at: 493 1204 3331 3457 3999 493 Size: 711 2127 126 542 2702 Fragments arranged by size: 2702 2127 711 542 126 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3261 3339 4015 4397 Size: 53 83 186 936 1728 78 676 382 Cuts at: 4397 5032 275 Size: 635 1451 Fragments arranged by size: 1728 1451 936 676 635 382 186 83 78 53 BsaWI w'CCGG_w Cuts at: 2157 4641 5039 5313 5460 5871 2157 Size: 2484 398 274 147 411 2494 Fragments arranged by size: 2494 2484 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2453 2483 2511 Size: 30 165 30 687 30 333 30 28 Cuts at: 2511 2541 1178 Size: 30 4845 Fragments arranged by size: 4845 687 333 165 30 30 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2995 1186 Size: 1809 4399 BseYI C'CCAG_C Cuts at: 3139 5411 5907 3139 Size: 2272 496 3440 Fragments arranged by size: 3440 2272 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2735 2987 1163 Size: 573 999 252 4384 Fragments arranged by size: 4384 999 573 252 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2319 3658 4419 5447 1540 Size: 275 186 318 1339 761 1028 2301 Fragments arranged by size: 2301 1339 1028 761 318 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2466 2709 2733 2753 2808 3330 4269 4354 Size: 358 243 24 20 55 522 939 85 Cuts at: 4354 5425 2108 Size: 1071 2891 Fragments arranged by size: 2891 1071 939 522 358 243 85 55 24 20 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 2672 3433 2672 Size: 761 5447 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2466 2474 2509 2709 2733 2753 Size: 399 318 358 8 35 200 24 20 Cuts at: 2753 2808 3254 3330 3507 3527 4269 4354 5425 Size: 55 446 76 177 20 742 85 1071 Cuts at: 5425 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 446 399 358 318 200 177 85 76 55 35 24 20 20 8 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3147 3563 844 Size: 2303 416 3489 Fragments arranged by size: 3489 2303 416 BsrBI CCG'CTC Cuts at: 1394 1823 2395 4095 1394 Size: 429 572 1700 3507 Fragments arranged by size: 3507 1700 572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2497 4703 4877 65 Size: 1183 1249 2206 174 1396 Fragments arranged by size: 2206 1396 1249 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2157 2314 2493 2916 2925 4852 5039 Size: 110 347 157 179 423 9 1927 187 Cuts at: 5039 1700 Size: 2869 Fragments arranged by size: 2869 1927 423 347 187 179 157 110 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 2225 2465 2540 2708 4268 5280 2225 Size: 240 75 168 1560 1012 3153 Fragments arranged by size: 3153 1560 1012 240 168 75 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 2722 794 Size: 1919 9 4280 Fragments arranged by size: 4280 1919 9 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 3292 1184 Size: 2108 4100 BstYI r'GATC_y Cuts at: 1972 2592 3075 3538 3905 4290 4307 5759 5845 Size: 620 483 463 367 385 17 1452 86 Cuts at: 5845 5857 6203 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 620 483 463 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4717 Fragments arranged by size: 4717 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3020 3382 487 Size: 683 56 1794 362 3313 Fragments arranged by size: 3313 1794 683 362 56 BtsI GCAGTG_nn' Cuts at: 2153 2723 3724 4518 4538 2153 Size: 570 1001 794 20 3823 Fragments arranged by size: 3823 1001 794 570 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2611 2646 529 Size: 35 2047 35 4091 Fragments arranged by size: 4091 2047 35 35 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DraIII CAC_nnn'GTG Cuts at: 2713 2713 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5215 809 Size: 507 3899 1802 Fragments arranged by size: 3899 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2152 2178 2316 Size: 54 1425 50 275 18 322 26 138 Cuts at: 2316 2736 2841 2892 3115 3655 4544 6173 8 Size: 420 105 51 223 540 889 1629 43 Fragments arranged by size: 1629 1425 889 540 420 322 275 223 138 105 54 51 50 43 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2316 3655 1537 Size: 275 504 1339 4090 Fragments arranged by size: 4090 1339 504 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3236 3422 4138 1353 Size: 1883 186 716 3423 Fragments arranged by size: 3423 1883 716 186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2098 2423 4795 5169 5315 387 Size: 1064 138 509 325 2372 374 146 1280 Fragments arranged by size: 2372 1280 1064 509 374 325 146 138 EcoICRI GAG'CTC Cuts at: 2751 2806 2751 Size: 55 6153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2480 2780 2849 2996 3328 4271 Size: 393 428 420 300 69 147 332 943 Cuts at: 4271 4868 5655 5923 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 943 787 597 428 420 393 332 300 268 147 69 EcoO109I rG'GnC_Cy Cuts at: 2133 2352 2998 3250 2133 Size: 219 646 252 5091 Fragments arranged by size: 5091 646 252 219 EcoRI G'AATT_C Cuts at: 3448 3517 3895 3448 Size: 69 378 5761 Fragments arranged by size: 5761 378 69 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2578 2791 3313 2578 Size: 213 522 5473 Fragments arranged by size: 5473 522 213 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2455 3100 5355 1678 Size: 777 645 2255 2531 Fragments arranged by size: 2531 2255 777 645 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2395 2427 4849 4881 4923 Size: 32 135 32 823 32 2422 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2626 2422 823 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3385 3552 3798 669 Size: 988 281 1447 167 246 3079 Fragments arranged by size: 3079 1447 988 281 246 167 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5322 5506 1305 Size: 4017 184 2007 Fragments arranged by size: 4017 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2154 2180 2738 2894 10 Size: 54 1425 343 322 26 558 156 3324 Fragments arranged by size: 3324 1425 558 343 322 156 54 26 MslI CAynn'nnrTG Cuts at: 518 1166 4166 4525 4684 518 Size: 648 3000 359 159 2042 Fragments arranged by size: 3000 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 2974 3624 4301 5449 5694 1606 Size: 782 586 650 677 1148 245 2120 Fragments arranged by size: 2120 1148 782 677 650 586 245 NaeI GCC'GGC Cuts at: 2316 2495 2918 2316 Size: 179 423 5606 Fragments arranged by size: 5606 423 179 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4717 Fragments arranged by size: 4717 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2314 2493 2916 2314 Size: 179 423 5606 Fragments arranged by size: 5606 423 179 NheI G'CTAG_C Cuts at: 1052 2015 1052 Size: 963 5245 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PmlI CAC'GTG Cuts at: 3331 3331 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2352 2998 2352 Size: 646 5562 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspOMI G'GGCC_C Cuts at: 3250 3250 Size: 6208 PstI C_TGCA'G Cuts at: 830 2014 2117 2814 2915 3560 830 Size: 1184 103 697 101 645 3478 Fragments arranged by size: 3478 1184 697 645 103 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2974 2974 Size: 6208 SacI G_AGCT'C Cuts at: 2753 2808 2753 Size: 55 6153 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SanDI GG'GwC_CC Cuts at: 2352 2352 Size: 6208 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SfcI C'TryA_G Cuts at: 826 1045 2010 2048 2113 2810 2911 3556 4691 Size: 219 965 38 65 697 101 645 1135 Cuts at: 4691 5372 5563 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 965 697 681 645 219 191 101 65 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2600 3535 1872 Size: 728 935 4545 Fragments arranged by size: 4545 935 728 SmlI C'TyrA_G Cuts at: 820 1017 1943 2675 2993 4312 5213 5475 820 Size: 197 926 732 318 1319 901 262 1553 Fragments arranged by size: 1553 1319 926 901 732 318 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 2427 2909 1517 Size: 910 482 4816 Fragments arranged by size: 4816 910 482 StyI C'CwwG_G Cuts at: 513 1065 2004 2437 3647 513 Size: 552 939 433 1210 3074 Fragments arranged by size: 3074 1210 939 552 433 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 4235 4252 4405 4590 5035 325 Size: 1255 840 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1498 1255 840 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4454 6166 96 Size: 274 80 33 51 1443 2477 1712 138 Fragments arranged by size: 2477 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2453 2485 2511 2543 6168 6200 2453 Size: 32 26 32 3625 32 2461 Fragments arranged by size: 3625 2461 32 32 32 26 Tth111I GACn'n_nGTC Cuts at: 1316 2618 1316 Size: 1302 4906 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 2147 2071 Size: 76 6132 XhoI C'TCGA_G Cuts at: 1943 2675 1943 Size: 732 5476 XmaI C'CCGG_G Cuts at: 1870 2598 3533 1870 Size: 728 935 4545 Fragments arranged by size: 4545 935 728 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI