Restriction map for pFN21AE1042
 (Linear) (Six-base) MAPSORT of: KIEE1042.seq  Check: 6148  from: 1  to: 1503



KIEE1042.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:19  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1323    1503 
   Size:       1323     180
 
AgeI A'CCGG_T

Cuts at:      0     152    1503 
   Size:        152    1351
 
AhdI GACnn_n'nnGTC

Cuts at:      0     404    1503 
   Size:        404    1099
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     525    1419    1503 
   Size:        525     894      84

  Fragments arranged by size: 

                 894     525      84
 
AlwNI CAG_nnn'CTG

Cuts at:      0     717     969    1503 
   Size:        717     252     534

  Fragments arranged by size: 

                 717     534     252
 
ApaI G_GGCC'C

Cuts at:      0    1249    1503 
   Size:       1249     254
 
ApoI r'AATT_y

Cuts at:      0    1443    1503 
   Size:       1443      60
 
AvaI C'yCGr_G

Cuts at:      0     593     670    1503 
   Size:        593      77     833

  Fragments arranged by size: 

                 833     593      77
 
BanI G'GyrC_C

Cuts at:      0     501    1198    1503 
   Size:        501     697     305

  Fragments arranged by size: 

                 697     501     305
 
BanII G_rGCy'C

Cuts at:      0     748     803    1249    1502    1503 
   Size:        748      55     446     253       1

  Fragments arranged by size: 

                 748     446     253      55       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     838    1050    1503 
   Size:        838     212     453

  Fragments arranged by size: 

                 838     453     212
 
BbvCI CC'TCA_GC

Cuts at:      0     184    1503 
   Size:        184    1319
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     626     660    1503 
   Size:        626      34     843

  Fragments arranged by size: 

                 843     626      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     377    1503 
   Size:        377    1126
 
BglII A'GATC_T

Cuts at:      0    1070    1503 
   Size:       1070     433
 
Bme1580I G_kGCm'C

Cuts at:      0     469     504    1249    1503 
   Size:        469      35     745     254

  Fragments arranged by size: 

                 745     469     254      35
 
BmgBI CAC'GTC

Cuts at:      0     522     615    1350    1503 
   Size:        522      93     735     153

  Fragments arranged by size: 

                 735     522     153      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     317    1503 
   Size:        317    1186
 
BmtI G_CTAG'C

Cuts at:      0      14    1503 
   Size:         14    1489
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     475     775     844     991    1503 
   Size:         55     420     300      69     147     512

  Fragments arranged by size: 

                 512     420     300     147      69      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     184     481    1503 
   Size:        184     297    1022

  Fragments arranged by size: 

                1022     297     184
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     973    1503 
   Size:        973     530
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     770    1503 
   Size:        770     733
 
BsaAI yAC'GTr

Cuts at:      0    1326    1452    1503 
   Size:       1326     126      51

  Fragments arranged by size: 

                1326     126      51
 
BsaHI Gr'CG_yC

Cuts at:      0    1256    1334    1503 
   Size:       1256      78     169

  Fragments arranged by size: 

                1256     169      78
 
BsaWI w'CCGG_w

Cuts at:      0     152    1503 
   Size:        152    1351
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      85     115     448     478     506     536    1503 
   Size:         85      30     333      30      28      30     967

  Fragments arranged by size: 

                 967     333      85      30      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     990    1503 
   Size:        990     513
 
BseYI C'CCAG_C

Cuts at:      0    1134    1503 
   Size:       1134     369
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     982    1503 
   Size:        730     252     521

  Fragments arranged by size: 

                 730     521     252
 
BsiEI CG_ry'CG

Cuts at:      0     314    1503 
   Size:        314    1189
 
BsiHKAI G_wGCw'C

Cuts at:      0     103     461     704     728     748     803    1325    1503 
   Size:        103     358     243      24      20      55     522     178

  Fragments arranged by size: 

                 522     358     243     178     103      55      24      20
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     667    1428    1503 
   Size:        667     761      75

  Fragments arranged by size: 

                 761     667      75
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     461     469     504     704     728     748     803 
   Size:        103     358       8      35     200      24      20      55

Cuts at:    803    1249    1325    1502    1503 
   Size:        446      76     177       1

  Fragments arranged by size: 

                 446     358     200     177     103      76      55      35
                  24      20       8       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1142    1503 
   Size:       1142     361
 
BsrBI CCG'CTC

Cuts at:      0     390    1503 
   Size:        390    1113
 
BsrDI GCAATG_nn'

Cuts at:      0     492    1503 
   Size:        492    1011
 
BsrFI r'CCGG_y

Cuts at:      0     152     309     488     911     920    1503 
   Size:        152     157     179     423       9     583

  Fragments arranged by size: 

                 583     423     179     157     152       9
 
BssSI C'ACGA_G

Cuts at:      0     220     460     535     703    1503 
   Size:        220     240      75     168     800

  Fragments arranged by size: 

                 800     240     220     168      75
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     708     717    1503 
   Size:        708       9     786

  Fragments arranged by size: 

                 786     708       9
 
BstEII G'GTnAC_C

Cuts at:      0    1287    1503 
   Size:       1287     216
 
BstYI r'GATC_y

Cuts at:      0     587    1070    1503 
   Size:        587     483     433

  Fragments arranged by size: 

                 587     483     433
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1015    1377    1503 
   Size:       1015     362     126

  Fragments arranged by size: 

                1015     362     126
 
BtsI GCAGTG_nn'

Cuts at:      0     148     718    1503 
   Size:        148     570     785

  Fragments arranged by size: 

                 785     570     148
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     606     641    1503 
   Size:        606      35     862

  Fragments arranged by size: 

                 862     606      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     708    1503 
   Size:        708     795
 
EaeI y'GGCC_r

Cuts at:      0     147     173     311     731     836     887    1110    1503 
   Size:        147      26     138     420     105      51     223     393

  Fragments arranged by size: 

                 420     393     223     147     138     105      51      26
            
 
EagI C'GGCC_G

Cuts at:      0     311    1503 
   Size:        311    1192
 
EarI CTCTTCn'nnn_

Cuts at:      0    1231    1417    1503 
   Size:       1231     186      86

  Fragments arranged by size: 

                1231     186      86
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      93     418    1503 
   Size:         93     325    1085

  Fragments arranged by size: 

                1085     325      93
 
EcoICRI GAG'CTC

Cuts at:      0     746     801    1503 
   Size:        746      55     702

  Fragments arranged by size: 

                 746     702      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     475     775     844     991    1323    1503 
   Size:         55     420     300      69     147     332     180

  Fragments arranged by size: 

                 420     332     300     180     147      69      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     128     347     993    1245    1503 
   Size:        128     219     646     252     258

  Fragments arranged by size: 

                 646     258     252     219     128
 
EcoRI G'AATT_C

Cuts at:      0    1443    1503 
   Size:       1443      60
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     573     786    1308    1503 
   Size:        573     213     522     195

  Fragments arranged by size: 

                 573     522     213     195
 
HaeII r_GCGC'y

Cuts at:      0     450    1095    1503 
   Size:        450     645     408

  Fragments arranged by size: 

                 645     450     408
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     390     422    1503 
   Size:        390      32    1081

  Fragments arranged by size: 

                1081     390      32
 
HincII GTy'rAC

Cuts at:      0    1380    1503 
   Size:       1380     123
 
MscI TGG'CCA

Cuts at:      0     149     175     733     889    1503 
   Size:        149      26     558     156     614

  Fragments arranged by size: 

                 614     558     156     149      26
 
MspA1I CmG'CkG

Cuts at:      0     383     969    1503 
   Size:        383     586     534

  Fragments arranged by size: 

                 586     534     383
 
NaeI GCC'GGC

Cuts at:      0     311     490     913    1503 
   Size:        311     179     423     590

  Fragments arranged by size: 

                 590     423     311     179
 
NgoMIV G'CCGG_C

Cuts at:      0     309     488     911    1503 
   Size:        309     179     423     592

  Fragments arranged by size: 

                 592     423     309     179
 
NheI G'CTAG_C

Cuts at:      0      10    1503 
   Size:         10    1493
 
PmlI CAC'GTG

Cuts at:      0    1326    1503 
   Size:       1326     177
 
PpuMI rG'GwC_Cy

Cuts at:      0     347     993    1503 
   Size:        347     646     510

  Fragments arranged by size: 

                 646     510     347
 
PspOMI G'GGCC_C

Cuts at:      0    1245    1503 
   Size:       1245     258
 
PstI C_TGCA'G

Cuts at:      0       9     112     809     910    1503 
   Size:          9     103     697     101     593

  Fragments arranged by size: 

                 697     593     103     101       9
 
PvuII CAG'CTG

Cuts at:      0     969    1503 
   Size:        969     534
 
SacI G_AGCT'C

Cuts at:      0     748     803    1503 
   Size:        748      55     700

  Fragments arranged by size: 

                 748     700      55
 
SanDI GG'GwC_CC

Cuts at:      0     347    1503 
   Size:        347    1156
 
SfcI C'TryA_G

Cuts at:      0       5      43     108     805     906    1503 
   Size:          5      38      65     697     101     597

  Fragments arranged by size: 

                 697     597     101      65      38       5
 
SmaI CCC'GGG

Cuts at:      0     595    1503 
   Size:        595     908
 
SmlI C'TyrA_G

Cuts at:      0     670     988    1503 
   Size:        670     318     515

  Fragments arranged by size: 

                 670     515     318
 
StuI AGG'CCT

Cuts at:      0     422     904    1503 
   Size:        422     482     599

  Fragments arranged by size: 

                 599     482     422
 
StyI C'CwwG_G

Cuts at:      0     432    1503 
   Size:        432    1071
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     415    1503 
   Size:        415    1088
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     448     480     506     538    1503 
   Size:        448      32      26      32     965

  Fragments arranged by size: 

                 965     448      32      32      26
 
Tth111I GACn'n_nGTC

Cuts at:      0     613    1503 
   Size:        613     890
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      66     142    1503 
   Size:         66      76    1361

  Fragments arranged by size: 

                1361      76      66
 
XhoI C'TCGA_G

Cuts at:      0     670    1503 
   Size:        670     833
 
XmaI C'CCGG_G

Cuts at:      0     593    1503 
   Size:        593     910
 

 Enzymes that do cut:

     AcuI     AgeI     AhdI     AlfI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI     BcgI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
   BstEII    BstYI    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII     MscI   MspA1I     NaeI   NgoMIV     NheI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BciVI     BclI    BfrBI     BlpI     BplI    BsaBI
    BsiWI     BsmI    BspEI    BspHI    BsrGI   BssHII    BstBI    BstXI
  BstZ17I   Bsu36I     BtgI     ClaI     DraI     DrdI    EcoNI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NarI     NcoI     NdeI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     XbaI     XmnI     ZraI
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