Restriction map for
pFN21AE1042
(Linear) (Six-base) MAPSORT of: KIEE1042.seq Check: 6148 from: 1 to: 1503
KIEE1042.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1323 1503
Size: 1323 180
AgeI A'CCGG_T
Cuts at: 0 152 1503
Size: 152 1351
AhdI GACnn_n'nnGTC
Cuts at: 0 404 1503
Size: 404 1099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 1419 1503
Size: 525 894 84
Fragments arranged by size:
894 525 84
AlwNI CAG_nnn'CTG
Cuts at: 0 717 969 1503
Size: 717 252 534
Fragments arranged by size:
717 534 252
ApaI G_GGCC'C
Cuts at: 0 1249 1503
Size: 1249 254
ApoI r'AATT_y
Cuts at: 0 1443 1503
Size: 1443 60
AvaI C'yCGr_G
Cuts at: 0 593 670 1503
Size: 593 77 833
Fragments arranged by size:
833 593 77
BanI G'GyrC_C
Cuts at: 0 501 1198 1503
Size: 501 697 305
Fragments arranged by size:
697 501 305
BanII G_rGCy'C
Cuts at: 0 748 803 1249 1502 1503
Size: 748 55 446 253 1
Fragments arranged by size:
748 446 253 55 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 838 1050 1503
Size: 838 212 453
Fragments arranged by size:
838 453 212
BbvCI CC'TCA_GC
Cuts at: 0 184 1503
Size: 184 1319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 626 660 1503
Size: 626 34 843
Fragments arranged by size:
843 626 34
BglI GCCn_nnn'nGGC
Cuts at: 0 377 1503
Size: 377 1126
BglII A'GATC_T
Cuts at: 0 1070 1503
Size: 1070 433
Bme1580I G_kGCm'C
Cuts at: 0 469 504 1249 1503
Size: 469 35 745 254
Fragments arranged by size:
745 469 254 35
BmgBI CAC'GTC
Cuts at: 0 522 615 1350 1503
Size: 522 93 735 153
Fragments arranged by size:
735 522 153 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 317 1503
Size: 317 1186
BmtI G_CTAG'C
Cuts at: 0 14 1503
Size: 14 1489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 475 775 844 991 1503
Size: 55 420 300 69 147 512
Fragments arranged by size:
512 420 300 147 69 55
Bpu10I CC'TnA_GC
Cuts at: 0 184 481 1503
Size: 184 297 1022
Fragments arranged by size:
1022 297 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 1503
Size: 973 530
BsaI GGTCTCn'nnnn_
Cuts at: 0 770 1503
Size: 770 733
BsaAI yAC'GTr
Cuts at: 0 1326 1452 1503
Size: 1326 126 51
Fragments arranged by size:
1326 126 51
BsaHI Gr'CG_yC
Cuts at: 0 1256 1334 1503
Size: 1256 78 169
Fragments arranged by size:
1256 169 78
BsaWI w'CCGG_w
Cuts at: 0 152 1503
Size: 152 1351
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 448 478 506 536 1503
Size: 85 30 333 30 28 30 967
Fragments arranged by size:
967 333 85 30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 990 1503
Size: 990 513
BseYI C'CCAG_C
Cuts at: 0 1134 1503
Size: 1134 369
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 982 1503
Size: 730 252 521
Fragments arranged by size:
730 521 252
BsiEI CG_ry'CG
Cuts at: 0 314 1503
Size: 314 1189
BsiHKAI G_wGCw'C
Cuts at: 0 103 461 704 728 748 803 1325 1503
Size: 103 358 243 24 20 55 522 178
Fragments arranged by size:
522 358 243 178 103 55 24 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1428 1503
Size: 667 761 75
Fragments arranged by size:
761 667 75
Bsp1286I G_dGCh'C
Cuts at: 0 103 461 469 504 704 728 748 803
Size: 103 358 8 35 200 24 20 55
Cuts at: 803 1249 1325 1502 1503
Size: 446 76 177 1
Fragments arranged by size:
446 358 200 177 103 76 55 35
24 20 8 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1142 1503
Size: 1142 361
BsrBI CCG'CTC
Cuts at: 0 390 1503
Size: 390 1113
BsrDI GCAATG_nn'
Cuts at: 0 492 1503
Size: 492 1011
BsrFI r'CCGG_y
Cuts at: 0 152 309 488 911 920 1503
Size: 152 157 179 423 9 583
Fragments arranged by size:
583 423 179 157 152 9
BssSI C'ACGA_G
Cuts at: 0 220 460 535 703 1503
Size: 220 240 75 168 800
Fragments arranged by size:
800 240 220 168 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 717 1503
Size: 708 9 786
Fragments arranged by size:
786 708 9
BstEII G'GTnAC_C
Cuts at: 0 1287 1503
Size: 1287 216
BstYI r'GATC_y
Cuts at: 0 587 1070 1503
Size: 587 483 433
Fragments arranged by size:
587 483 433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1015 1377 1503
Size: 1015 362 126
Fragments arranged by size:
1015 362 126
BtsI GCAGTG_nn'
Cuts at: 0 148 718 1503
Size: 148 570 785
Fragments arranged by size:
785 570 148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 606 641 1503
Size: 606 35 862
Fragments arranged by size:
862 606 35
DraIII CAC_nnn'GTG
Cuts at: 0 708 1503
Size: 708 795
EaeI y'GGCC_r
Cuts at: 0 147 173 311 731 836 887 1110 1503
Size: 147 26 138 420 105 51 223 393
Fragments arranged by size:
420 393 223 147 138 105 51 26
EagI C'GGCC_G
Cuts at: 0 311 1503
Size: 311 1192
EarI CTCTTCn'nnn_
Cuts at: 0 1231 1417 1503
Size: 1231 186 86
Fragments arranged by size:
1231 186 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 93 418 1503
Size: 93 325 1085
Fragments arranged by size:
1085 325 93
EcoICRI GAG'CTC
Cuts at: 0 746 801 1503
Size: 746 55 702
Fragments arranged by size:
746 702 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 475 775 844 991 1323 1503
Size: 55 420 300 69 147 332 180
Fragments arranged by size:
420 332 300 180 147 69 55
EcoO109I rG'GnC_Cy
Cuts at: 0 128 347 993 1245 1503
Size: 128 219 646 252 258
Fragments arranged by size:
646 258 252 219 128
EcoRI G'AATT_C
Cuts at: 0 1443 1503
Size: 1443 60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 573 786 1308 1503
Size: 573 213 522 195
Fragments arranged by size:
573 522 213 195
HaeII r_GCGC'y
Cuts at: 0 450 1095 1503
Size: 450 645 408
Fragments arranged by size:
645 450 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 390 422 1503
Size: 390 32 1081
Fragments arranged by size:
1081 390 32
HincII GTy'rAC
Cuts at: 0 1380 1503
Size: 1380 123
MscI TGG'CCA
Cuts at: 0 149 175 733 889 1503
Size: 149 26 558 156 614
Fragments arranged by size:
614 558 156 149 26
MspA1I CmG'CkG
Cuts at: 0 383 969 1503
Size: 383 586 534
Fragments arranged by size:
586 534 383
NaeI GCC'GGC
Cuts at: 0 311 490 913 1503
Size: 311 179 423 590
Fragments arranged by size:
590 423 311 179
NgoMIV G'CCGG_C
Cuts at: 0 309 488 911 1503
Size: 309 179 423 592
Fragments arranged by size:
592 423 309 179
NheI G'CTAG_C
Cuts at: 0 10 1503
Size: 10 1493
PmlI CAC'GTG
Cuts at: 0 1326 1503
Size: 1326 177
PpuMI rG'GwC_Cy
Cuts at: 0 347 993 1503
Size: 347 646 510
Fragments arranged by size:
646 510 347
PspOMI G'GGCC_C
Cuts at: 0 1245 1503
Size: 1245 258
PstI C_TGCA'G
Cuts at: 0 9 112 809 910 1503
Size: 9 103 697 101 593
Fragments arranged by size:
697 593 103 101 9
PvuII CAG'CTG
Cuts at: 0 969 1503
Size: 969 534
SacI G_AGCT'C
Cuts at: 0 748 803 1503
Size: 748 55 700
Fragments arranged by size:
748 700 55
SanDI GG'GwC_CC
Cuts at: 0 347 1503
Size: 347 1156
SfcI C'TryA_G
Cuts at: 0 5 43 108 805 906 1503
Size: 5 38 65 697 101 597
Fragments arranged by size:
697 597 101 65 38 5
SmaI CCC'GGG
Cuts at: 0 595 1503
Size: 595 908
SmlI C'TyrA_G
Cuts at: 0 670 988 1503
Size: 670 318 515
Fragments arranged by size:
670 515 318
StuI AGG'CCT
Cuts at: 0 422 904 1503
Size: 422 482 599
Fragments arranged by size:
599 482 422
StyI C'CwwG_G
Cuts at: 0 432 1503
Size: 432 1071
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 1503
Size: 415 1088
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 448 480 506 538 1503
Size: 448 32 26 32 965
Fragments arranged by size:
965 448 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 0 613 1503
Size: 613 890
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 142 1503
Size: 66 76 1361
Fragments arranged by size:
1361 76 66
XhoI C'TCGA_G
Cuts at: 0 670 1503
Size: 670 833
XmaI C'CCGG_G
Cuts at: 0 593 1503
Size: 593 910
Enzymes that do cut:
AcuI AgeI AhdI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstYI BtgZI BtsI CspCI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII MscI MspA1I NaeI NgoMIV NheI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SfcI SmaI
SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BlpI BplI BsaBI
BsiWI BsmI BspEI BspHI BsrGI BssHII BstBI BstXI
BstZ17I Bsu36I BtgI ClaI DraI DrdI EcoNI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NcoI NdeI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XmnI ZraI