(Circular) (Six-base) MAPSORT of: pFN21AE4488.seq Check: 2281 from: 1 to: 6235 pFN21AE4488 6235 bp With 182 enzymes: SgfI * March 28, 2012 13:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3339 3339 Size: 6235 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 3578 1937 Size: 1497 144 4594 Fragments arranged by size: 4594 1497 144 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4298 5682 5950 1632 Size: 2666 1384 268 1917 Fragments arranged by size: 2666 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 3978 5550 3978 Size: 1572 4663 ApaI G_GGCC'C Cuts at: 2209 2411 2209 Size: 202 6033 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 2241 2687 2844 2879 2921 3226 3527 3544 Size: 748 446 157 35 42 305 301 17 Cuts at: 3544 3770 3922 6031 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 748 446 305 301 226 157 152 42 35 17 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 3560 1870 Size: 73 1617 4545 Fragments arranged by size: 4545 1617 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3165 3198 3165 Size: 33 6202 BamHI G'GATC_C Cuts at: 2012 3565 3932 2012 Size: 1553 367 4315 Fragments arranged by size: 4315 1553 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3556 5011 618 Size: 325 217 627 1769 1455 1842 Fragments arranged by size: 1842 1769 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2209 2411 3554 1391 Size: 818 202 1143 4072 Fragments arranged by size: 4072 1143 818 202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3166 4127 5337 3166 Size: 961 1210 4064 Fragments arranged by size: 4064 1210 961 BclI T'GATC_A Cuts at: 1548 2649 1548 Size: 1101 5134 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2274 4846 136 Size: 107 122 71 631 605 602 2572 1525 Fragments arranged by size: 2572 1525 631 605 602 122 107 71 BglII A'GATC_T Cuts at: 6230 6230 Size: 6235 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2199 2209 2411 4296 5452 1790 Size: 409 10 202 1885 1156 2573 Fragments arranged by size: 2573 1885 1156 409 202 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 3966 4924 Size: 416 53 83 399 24 906 1643 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1643 958 906 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3130 3130 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2775 3303 4895 1239 Size: 1536 528 1592 2579 Fragments arranged by size: 2579 1592 1536 528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3253 4360 5225 5523 3253 Size: 1107 865 298 3965 Fragments arranged by size: 3965 1107 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2033 2103 3507 4898 882 Size: 617 534 70 1404 1391 2219 Fragments arranged by size: 2219 1404 1391 617 534 70 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3288 4042 4424 5059 Size: 53 83 186 936 1755 754 382 635 Cuts at: 5059 275 Size: 1451 Fragments arranged by size: 1755 1451 936 754 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4668 5066 5340 5487 5898 4668 Size: 398 274 147 411 5005 Fragments arranged by size: 5005 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6010 Fragments arranged by size: 6010 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2121 3138 1186 Size: 935 1017 4283 Fragments arranged by size: 4283 1017 935 BseYI C'CCAG_C Cuts at: 5438 5934 5438 Size: 496 5739 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 2706 3317 3386 1163 Size: 573 462 508 611 69 4012 Fragments arranged by size: 4012 611 573 508 462 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2080 2121 3685 4446 5474 Size: 275 186 47 32 41 1564 761 1028 Cuts at: 5474 1540 Size: 2301 Fragments arranged by size: 2301 1564 1028 761 275 186 47 41 32 BsiHKAI G_wGCw'C Cuts at: 2889 3330 4296 4381 5452 2889 Size: 441 966 85 1071 3672 Fragments arranged by size: 3672 1071 966 441 85 BsmI GAATG_Cn' Cuts at: 2224 3138 3267 3746 3839 2224 Size: 914 129 479 93 4620 Fragments arranged by size: 4620 914 479 129 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 2209 2411 2889 3330 3554 4296 Size: 399 409 10 202 478 441 224 742 Cuts at: 4296 4381 5452 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 441 409 399 224 202 85 10 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3339 3590 844 Size: 2495 251 3489 Fragments arranged by size: 3489 2495 251 BsrBI CCG'CTC Cuts at: 1394 1823 4122 1394 Size: 429 2299 3507 Fragments arranged by size: 3507 2299 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4730 4904 65 Size: 1183 3482 174 1396 Fragments arranged by size: 3482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3051 4879 5066 1700 Size: 110 1241 1828 187 2869 Fragments arranged by size: 2869 1828 1241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 2176 794 Size: 1382 4853 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstYI r'GATC_y Cuts at: 1972 2012 3565 3932 4317 4334 5786 5872 5884 Size: 40 1553 367 385 17 1452 86 12 Cuts at: 5884 6230 1972 Size: 346 1977 Fragments arranged by size: 1977 1553 1452 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2115 513 Size: 552 939 111 4633 Fragments arranged by size: 4633 939 552 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2117 487 Size: 683 56 891 4605 Fragments arranged by size: 4605 891 683 56 BtsI GCAGTG_nn' Cuts at: 3751 4545 4565 3751 Size: 794 20 5421 Fragments arranged by size: 5421 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 2262 3539 2262 Size: 1277 4958 DraIII CAC_nnn'GTG Cuts at: 2176 2176 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2117 2777 5242 809 Size: 507 801 660 2465 1802 Fragments arranged by size: 2465 1802 801 660 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 3682 4571 Size: 54 1425 50 275 18 215 1637 889 Cuts at: 4571 6200 8 Size: 1629 43 Fragments arranged by size: 1637 1629 1425 889 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2045 3682 1537 Size: 275 233 1637 4090 Fragments arranged by size: 4090 1637 275 233 EarI CTCTTCn'nnn_ Cuts at: 1353 2341 3100 4165 1353 Size: 988 759 1065 3423 Fragments arranged by size: 3423 1065 988 759 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4822 5196 5342 387 Size: 1064 138 3233 374 146 1280 Fragments arranged by size: 3233 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2775 3303 4298 4895 5682 5950 1239 Size: 393 1143 528 995 597 787 268 1524 Fragments arranged by size: 1524 1143 995 787 597 528 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2724 2724 Size: 6235 EcoO109I rG'GnC_Cy Cuts at: 2407 2408 2407 Size: 1 6234 EcoRI G'AATT_C Cuts at: 2921 3544 3922 2921 Size: 623 378 5234 Fragments arranged by size: 5234 623 378 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 5382 1678 Size: 3704 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4876 4908 4950 4982 1373 Size: 32 135 32 3304 32 42 32 2626 Fragments arranged by size: 3304 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2805 3579 3825 669 Size: 988 281 867 774 246 3079 Fragments arranged by size: 3079 988 867 774 281 246 HindIII A'AGCT_T Cuts at: 748 2559 748 Size: 1811 4424 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2425 5349 5533 1305 Size: 1120 2924 184 2007 Fragments arranged by size: 2924 2007 1120 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 4193 4552 4711 518 Size: 648 1375 1652 359 159 2042 Fragments arranged by size: 2042 1652 1375 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2117 2426 3046 3518 3651 4328 5476 Size: 444 67 309 620 472 133 677 1148 Cuts at: 5476 5721 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 620 472 444 309 245 133 67 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4744 Fragments arranged by size: 4744 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3593 5138 1132 Size: 97 2364 1545 2229 Fragments arranged by size: 2364 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6235 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6235 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 2205 2407 2205 Size: 202 6033 PstI C_TGCA'G Cuts at: 830 3587 830 Size: 2757 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2426 3046 3518 2426 Size: 620 472 5143 Fragments arranged by size: 5143 620 472 SacII CC_GC'GG Cuts at: 2118 2118 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SexAI A'CCwGG_T Cuts at: 2172 2172 Size: 6235 SfcI C'TryA_G Cuts at: 826 1045 2496 3583 4718 5399 5590 826 Size: 219 1451 1087 1135 681 191 1471 Fragments arranged by size: 1471 1451 1135 1087 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3232 4339 5240 5502 820 Size: 197 926 1289 1107 901 262 1553 Fragments arranged by size: 1553 1289 1107 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 2451 2970 4159 5 Size: 47 2399 519 1189 2081 Fragments arranged by size: 2399 2081 1189 519 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2004 2947 3674 513 Size: 552 939 943 727 3074 Fragments arranged by size: 3074 943 939 727 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 4262 4279 4432 4617 5062 325 Size: 1255 486 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 4481 6193 Size: 274 80 33 51 1443 168 2336 1712 Cuts at: 6193 96 Size: 138 Fragments arranged by size: 2336 1712 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6195 6227 6195 Size: 32 6203 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 4364 4364 Size: 6235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI