Restriction map for
pFN21AB7682
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI AspEI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HpaI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MscI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tsp509I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 339 GGTACC G^GTACC
AccIII 738 TCCGGA T^CCGGA
AcyI 12 GGCGCC G[GA]^CG[CT]C
Alw44I 1071 GTGCAC G^TGCAC
AlwNI 505 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1071 GTGCAC G^TGCAC
Asp700I 1053 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 339 GGTACC G^GTACC
AspHI 1071 GTGCAC G[AT]GC[AT]^C
AspI 686 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 12 GGCGCC GGCGC^C
BlpI 646 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 646 GCTCAGC GC^T[ACGT]AGC
BsaHI 12 GGCGCC G[GA]^CG[CT]C
BsaI 684 GAGACC GGTCTC(1/5)
BseAI 738 TCCGGA T^CCGGA
BseRI 674 CTCCTC GAGGAG(10/8)
BsgI 316 GTGCAG GTGCAG(16/14)
BsiHKAI 1071 GTGCAC G[AT]GC[AT]^C
BsmAI 684 GAGAC GTCTC(1/5)
BspEI 738 TCCGGA T^CCGGA
BsrFI 100 GCCGGT [GA]^CCGG[CT]
BstAPI 540 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1160 AGATCC [GA]^GATC[CT]
CelII 646 GCTCAGC GC^T[ACGT]AGC
Cfr10I 100 GCCGGT [GA]^CCGG[CT]
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 114 GAAGAG CTCTTC(1/4)
EcoNI 964 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 12 GGCGCC G[GA]^CG[CT]C
HincII 389 GTCGAC GT[CT]^[GA]AC
HindII 389 GTCGAC GT[CT]^[GA]AC
HindIII 1115 AAGCTT A^AGCTT
KasI 12 GGCGCC G^GCGCC
KpnI 339 GGTACC GGTAC^C
Ksp632I 114 GAAGAG CTCTTC(1/4)
MflI 1160 AGATCC [GA]^GATC[CT]
MroI 738 TCCGGA T^CCGGA
MseI 851 TTAA T^TAA
MslI 1074 CACAGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1195 CAGCTG C[AC]G^C[GT]G
NarI 12 GGCGCC GG^CGCC
NspI 437 ACATGC [GA]CATG^[CT]
PflFI 686 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 117 GAGTC GAGTC(4/5)
PpuMI 450 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1195 CAGCTG CAG^CTG
SalI 389 GTCGAC G^TCGAC
SfcI 351 CTACAG C^T[GA][CT]AG
SfoI 12 GGCGCC GGC^GCC
SmlI 1147 CTCAAG C^T[CT][GA]AG
StuI 1040 AGGCCT AGG^CCT
TaqI 390 TCGA T^CGA
TfiI 789 GAATC G^A[AT]TC
Tru9I 851 TTAA T^TAA
Tth111I 686 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 390 TCGA T^CGA
XcmI 605 CCAGAGCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1160 AGATCC [GA]^GATC[CT]
XmnI 1053 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 389 GTCGAC GT^[AC][GT]AC
1171 GTCTAC
AfaI 340 GTAC GT^AC
981 GTAC
AlwI 1161 GATCC GGATC(4/5)
207 GGATC
BglI 4 GCCGCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1123 GCCGTCTGGGC
BsaWI 24 ACCGGA [AT]^CCGG[AT]
738 TCCGGA
BspMI 795 ACCTGC ACCTGC(4/8)
940 GCAGGT
DsaI 200 CCATGG C^C[GA][CT]GG
590 CCATGG
HaeII 12 GGCGCC [GA]GCGC^[CT]
1090 AGCGCC
HinfI 117 GAGTC G^A[ACGT]TC
789 GAATC
MboII 114 GAAGA GAAGA(8/7)
235 GAAGA
NcoI 200 CCATGG C^CATGG
590 CCATGG
PflMI 277 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
504 CCAGCCTCTGG
RsaI 340 GTAC GT^AC
981 GTAC
SexAI 212 ACCTGGT A^CC[AT]GGT
687 ACCTGGT
Van91I 277 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
504 CCAGCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI ApaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TseI TspRI