(Circular) (Six-base) MAPSORT of: pFN21AB7682.seq Check: 1430 from: 1 to: 5923 pFN21AB7682 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2809 2936 2809 Size: 127 5796 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2395 3177 3266 1937 Size: 458 782 89 4594 Fragments arranged by size: 4594 782 458 89 Acc65I G'GTAC_C Cuts at: 2344 3244 2344 Size: 900 5023 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3138 3986 5370 5638 1632 Size: 1506 848 1384 268 1917 Fragments arranged by size: 1917 1506 1384 848 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2315 1184 Size: 1131 4792 AlwNI CAG_nnn'CTG Cuts at: 2515 3666 5238 2515 Size: 1151 1572 3200 Fragments arranged by size: 3200 1572 1151 ApaI G_GGCC'C Cuts at: 2162 2381 2432 2561 2627 2964 2162 Size: 219 51 129 66 337 5121 Fragments arranged by size: 5121 337 219 129 66 51 ApaLI G'TGCA_C Cuts at: 3076 3980 5136 3076 Size: 904 1156 3863 Fragments arranged by size: 3863 1156 904 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 3248 1870 Size: 73 1305 4545 Fragments arranged by size: 4545 1305 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2344 2929 2996 3244 Size: 325 217 627 230 327 585 67 248 Cuts at: 3244 4699 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 585 327 325 248 230 217 67 BanII G_rGCy'C Cuts at: 1391 2162 2257 2381 2432 2561 2627 2665 2706 Size: 771 95 124 51 129 66 38 41 Cuts at: 2706 2829 2957 2964 3242 1391 Size: 123 128 7 278 4072 Fragments arranged by size: 4072 771 278 129 128 124 123 95 66 51 41 38 7 BbeI G_GCGC'C Cuts at: 2021 2021 Size: 5923 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 3134 4534 Size: 107 122 71 631 605 343 1119 1400 Cuts at: 4534 136 Size: 1525 Fragments arranged by size: 1525 1400 1119 631 605 343 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 2652 3340 2652 Size: 688 5235 Bme1580I G_kGCm'C Cuts at: 1790 2162 2304 2381 2432 2561 2627 2909 2964 Size: 372 142 77 51 129 66 282 55 Cuts at: 2964 3080 3984 5140 1790 Size: 116 904 1156 2573 Fragments arranged by size: 2573 1156 904 372 282 142 129 116 77 66 55 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2532 2555 2865 Size: 416 53 83 399 24 1115 23 310 Cuts at: 2865 3654 4612 442 Size: 789 958 1753 Fragments arranged by size: 1753 1115 958 789 416 399 310 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2848 2848 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 2385 2669 2895 4583 1239 Size: 821 325 284 226 1688 2579 Fragments arranged by size: 2579 1688 821 325 284 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3137 4048 4913 5211 3137 Size: 911 865 298 3849 Fragments arranged by size: 3849 911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2683 4586 882 Size: 617 1184 1903 2219 Fragments arranged by size: 2219 1903 1184 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2018 3730 4112 4747 Size: 53 83 186 936 485 1712 382 635 Cuts at: 4747 275 Size: 1451 Fragments arranged by size: 1712 1451 936 635 485 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2029 2743 4356 4754 5028 5175 5586 2029 Size: 714 1613 398 274 147 411 2366 Fragments arranged by size: 2366 1613 714 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2822 2852 3155 3185 1178 Size: 30 165 30 1419 30 303 30 3916 Fragments arranged by size: 3916 1419 303 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2670 1186 Size: 1484 4439 BseYI C'CCAG_C Cuts at: 2078 2382 2492 2620 2708 2964 5126 5622 2078 Size: 304 110 128 88 256 2162 496 2379 Fragments arranged by size: 2379 2162 496 304 256 128 110 88 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 1163 Size: 573 606 4744 Fragments arranged by size: 4744 606 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3080 3984 4069 5140 3080 Size: 904 85 1071 3863 Fragments arranged by size: 3863 1071 904 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2257 2304 2381 2432 2561 2627 Size: 399 372 95 47 77 51 129 66 Cuts at: 2627 2665 2706 2829 2909 2957 2964 3080 3242 Size: 38 41 123 80 48 7 116 162 Cuts at: 3242 3984 4069 5140 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 162 129 123 116 95 85 80 77 66 51 48 47 41 38 7 BspEI T'CCGG_A Cuts at: 2743 5586 2743 Size: 2843 3080 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2809 2936 3278 844 Size: 1965 127 342 3489 Fragments arranged by size: 3489 1965 342 127 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 4567 4754 1700 Size: 110 295 2462 187 2869 Fragments arranged by size: 2869 2462 295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2551 794 Size: 1757 4166 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2459 2693 2926 2425 Size: 34 234 233 5422 Fragments arranged by size: 5422 234 233 34 BstYI r'GATC_y Cuts at: 1972 3165 3253 3620 4005 4022 5474 5560 5572 Size: 1193 88 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1193 385 367 346 88 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2205 2595 513 Size: 552 939 201 390 3841 Fragments arranged by size: 3841 939 552 390 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2896 3439 4233 4253 2896 Size: 543 794 20 4566 Fragments arranged by size: 4566 794 543 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2929 2964 529 Size: 35 2365 35 3488 Fragments arranged by size: 3488 2365 35 35 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3370 4259 Size: 54 1425 50 275 18 177 1363 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1363 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 3853 1353 Size: 761 1739 3423 Fragments arranged by size: 3423 1739 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2385 2669 2895 3138 3986 4583 Size: 393 428 325 284 226 243 848 597 Cuts at: 4583 5370 5638 1239 Size: 787 268 1524 Fragments arranged by size: 1524 848 787 597 428 393 325 284 268 243 226 EcoNI CCTnn'n_nnAGG Cuts at: 2973 2973 Size: 5923 EcoO109I rG'GnC_Cy Cuts at: 2158 2159 2377 2378 2429 2456 2961 2158 Size: 1 218 1 51 27 505 5120 Fragments arranged by size: 5120 505 218 51 27 1 1 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2021 3099 5070 1678 Size: 343 1078 1971 2531 Fragments arranged by size: 2531 1971 1078 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2680 2712 3155 3187 4564 Size: 32 135 32 1108 32 443 32 1377 Cuts at: 4564 4596 4638 4670 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1377 1108 443 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2396 3267 3513 669 Size: 988 281 458 871 246 3079 Fragments arranged by size: 3079 988 871 458 281 246 HindIII A'AGCT_T Cuts at: 748 3120 748 Size: 2372 3551 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2017 2017 Size: 5923 KpnI G_GTAC'C Cuts at: 2348 3248 2348 Size: 900 5023 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 2984 5037 5221 1305 Size: 948 731 2053 184 2007 Fragments arranged by size: 2053 2007 948 731 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3083 3881 4240 4399 518 Size: 648 1917 798 359 159 2042 Fragments arranged by size: 2042 1917 798 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3202 3339 4016 5164 5409 1606 Size: 1596 137 677 1148 245 2120 Fragments arranged by size: 2120 1596 1148 677 245 137 NarI GG'CG_CC Cuts at: 2018 2018 Size: 5923 NcoI C'CATG_G Cuts at: 513 1065 2004 2205 2595 513 Size: 552 939 201 390 3841 Fragments arranged by size: 3841 939 552 390 201 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2446 3281 4826 1132 Size: 97 1217 835 1545 2229 Fragments arranged by size: 2229 1545 1217 835 97 PasI CC'CwG_GG Cuts at: 1348 2452 1348 Size: 1104 4819 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2288 2515 1108 Size: 1180 227 4516 Fragments arranged by size: 4516 1180 227 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2456 2456 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2158 2377 2428 2557 2623 2960 2158 Size: 219 51 129 66 337 5121 Fragments arranged by size: 5121 337 219 129 66 51 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 3202 3202 Size: 5923 SalI G'TCGA_C Cuts at: 1936 2394 3265 1936 Size: 458 871 4594 Fragments arranged by size: 4594 871 458 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 2217 2692 2217 Size: 475 5448 SfcI C'TryA_G Cuts at: 826 1045 2356 3271 4406 5087 5278 826 Size: 219 1311 915 1135 681 191 1471 Fragments arranged by size: 1471 1311 1135 915 681 219 191 SfoI GGC'GCC Cuts at: 2019 2019 Size: 5923 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3152 4027 4928 5190 820 Size: 197 926 1209 875 901 262 1553 Fragments arranged by size: 1553 1209 926 901 875 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 3047 1517 Size: 1530 4393 StyI C'CwwG_G Cuts at: 513 1065 2004 2205 2287 2595 3159 3362 513 Size: 552 939 201 82 308 564 203 3074 Fragments arranged by size: 3074 939 564 552 308 203 201 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 3950 3967 4120 4305 4750 325 Size: 1255 1025 1345 17 153 185 445 1498 Fragments arranged by size: 1498 1345 1255 1025 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2394 2426 2822 2854 5883 5915 2394 Size: 32 396 32 3029 32 2402 Fragments arranged by size: 3029 2402 396 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2694 1316 Size: 1378 4545 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2617 2617 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 3062 4052 3062 Size: 990 4933 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI