Restriction map for
pFN21AE6209
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccII AccIII AclI AflII
AflIII AgeI AhdI AlwNI AocI
ApaI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 637 GGTACC G^GTACC
AccI 700 GTCTAC GT^[AC][GT]AC
AcsI 555 AAATTC [GA]^AATT[CT]
Alw44I 232 GTGCAC G^TGCAC
Aor51HI 185 AGCGCT AGC^GCT
ApaLI 232 GTGCAC G^TGCAC
ApoI 555 AAATTC [GA]^AATT[CT]
Asp700I 288 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 637 GGTACC G^GTACC
AvaII 619 GGACC G^G[AT]CC
BbeI 97 GGCGCC GGCGC^C
BcnI 304 CCCGG CC^[GC]GG
BglI 299 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmAI 436 GTCTC GTCTC(1/5)
BsmFI 618 GGGAC GGGAC(10/14)
BspMI 129 GCAGGT ACCTGC(4/8)
BsrDI 602 GCAATG GCAATG(2/0)
BsrI 549 ACTGG ACTGG(1/-1)
DraIII 723 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 691 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 678 CGGCCA [CT]^GGCC[GA]
Eco47III 185 AGCGCT AGC^GCT
FauI 404 CCCGC CCCGC(4/6)
HgaI 170 GCGTC GACGC(5/10)
KasI 97 GGCGCC G^GCGCC
KpnI 637 GGTACC GGTAC^C
MseI 329 TTAA T^TAA
MspA1I 405 CCGCTG C[AC]G^C[GT]G
NarI 97 GGCGCC GG^CGCC
NciI 304 CCCGG CC^[GC]GG
SacI 266 GAGCTC GAGCT^C
SfoI 97 GGCGCC GGC^GCC
SmlI 707 CTCAAG C^T[CT][GA]AG
StuI 281 AGGCCT AGG^CCT
TaqI 286 TCGA T^CGA
Tru9I 329 TTAA T^TAA
Tsp509I 556 AATT ^AATT
TthHB8I 286 TCGA T^CGA
XmnI 288 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 97 GGCGCC G[GA]^CG[CT]C
169 GGCGTC
BanI 97 GGCGCC G^G[CT][GA]CC
637 GGTACC
BanII 266 GAGCTC G[GA]GC[CT]^C
459 GAGCCC
BbvI 396 GCTGC GCAGC(8/12)
407 GCTGC
BsaHI 97 GGCGCC G[GA]^CG[CT]C
169 GGCGTC
BsgI 525 CTGCAC GTGCAG(16/14)
630 GTGCAG
BsiYI 119 CCCACCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
123 CCCATGGCAGG
BslI 119 CCCACCCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
123 CCCATGGCAGG
BssSI 456 CACGAG CACGAG(-5/-1)
343 CTCGTG
BstYI 7 AGATCC [GA]^GATC[CT]
29 AGATCC
Cac8I 571 GCTGGC GC[ACGT]^[ACGT]GC
598 GCCTGC
Cfr13I 135 GGCCC G^G[ACGT]CC
619 GGACC
DsaI 124 CCATGG C^C[GA][CT]GG
377 CCATGG
HaeII 97 GGCGCC [GA]GCGC^[CT]
185 AGCGCT
HapII 305 CCGG C^CGG
440 CCGG
Hin1I 97 GGCGCC G[GA]^CG[CT]C
169 GGCGTC
HpaII 305 CCGG C^CGG
440 CCGG
MboII 67 GAAGA GAAGA(8/7)
384 GAAGA
MflI 7 AGATCC [GA]^GATC[CT]
29 AGATCC
MspI 305 CCGG C^CGG
440 CCGG
NcoI 124 CCATGG C^CATGG
377 CCATGG
PstI 25 CTGCAG CTGCA^G
59 CTGCAG
Sau96I 135 GGCCC G^G[ACGT]CC
619 GGACC
SfaNI 575 GCATC GCATC(5/9)
753 GCATC
SfcI 25 CTGCAG C^T[GA][CT]AG
59 CTGCAG
TseI 396 GCTGC G^C[AT]GC
407 GCTGC
XhoII 7 AGATCC [GA]^GATC[CT]
29 AGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BmyI BsaJI BsiHKAI Bsp1286I BssKI
BstF5I BstNI CfoI DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboI MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI StyI Tsp45I
TspRI