(Circular) (Six-base) MAPSORT of: pFN21AE6209.seq Check: 8146 from: 1 to: 5464 pFN21AE6209 5464 bp With 182 enzymes: SgfI * March 18, 2015 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2125 2125 Size: 5464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2706 2807 1937 Size: 769 101 4594 Fragments arranged by size: 4594 769 101 Acc65I G'GTAC_C Cuts at: 2642 2785 2642 Size: 143 5321 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3527 4911 5179 1632 Size: 1895 1384 268 1917 Fragments arranged by size: 1917 1895 1384 268 AfeI AGC'GCT Cuts at: 2192 2192 Size: 5464 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 2714 1166 Size: 1548 3916 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AlwNI CAG_nnn'CTG Cuts at: 3207 4779 3207 Size: 1572 3892 ApaLI G'TGCA_C Cuts at: 2237 3521 4677 2237 Size: 1284 1156 3024 Fragments arranged by size: 3024 1284 1156 ApoI r'AATT_y Cuts at: 1493 2560 2773 2999 3151 5260 1493 Size: 1067 213 226 152 2109 1697 Fragments arranged by size: 2109 1697 1067 226 213 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2789 1870 Size: 73 846 4545 Fragments arranged by size: 4545 846 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2642 2785 4240 618 Size: 325 217 627 315 540 143 1455 1842 Fragments arranged by size: 1842 1455 627 540 325 315 217 143 BanII G_rGCy'C Cuts at: 1391 2275 2468 2783 1391 Size: 884 193 315 4072 Fragments arranged by size: 4072 884 315 193 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 5464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2310 4075 136 Size: 107 122 71 631 605 638 1765 1525 Fragments arranged by size: 1765 1525 638 631 605 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 2241 2456 3525 4681 1790 Size: 451 215 1069 1156 2573 Fragments arranged by size: 2573 1156 1069 451 215 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 4153 442 Size: 416 53 83 399 24 1778 958 1753 Fragments arranged by size: 1778 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4124 1239 Size: 2885 2579 Bpu10I CC'TnA_GC Cuts at: 2050 2050 Size: 5464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2697 3589 4454 4752 2697 Size: 892 865 298 3409 Fragments arranged by size: 3409 892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4127 882 Size: 617 2628 2219 Fragments arranged by size: 2628 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 2175 3271 3653 Size: 53 83 186 936 570 72 1096 382 Cuts at: 3653 4288 275 Size: 635 1451 Fragments arranged by size: 1451 1096 936 635 570 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5464 BseYI C'CCAG_C Cuts at: 2193 2257 2448 4667 5163 2193 Size: 64 191 2219 496 2494 Fragments arranged by size: 2494 2219 496 191 64 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2515 2656 1163 Size: 573 779 141 3971 Fragments arranged by size: 3971 779 573 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 2241 2275 3525 3610 4681 2202 Size: 39 34 1250 85 1071 2985 Fragments arranged by size: 2985 1250 1071 85 39 34 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2241 2275 2456 2468 2783 3525 Size: 399 412 39 34 181 12 315 742 Cuts at: 3525 3610 4681 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 315 181 85 39 34 12 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2125 2819 844 Size: 1281 694 3489 Fragments arranged by size: 3489 1281 694 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2614 3959 4133 65 Size: 1183 1366 1345 174 1396 Fragments arranged by size: 1396 1366 1345 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4108 4295 1700 Size: 110 2298 187 2869 Fragments arranged by size: 2869 2298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 2348 2461 3524 4536 2348 Size: 113 1063 1012 3276 Fragments arranged by size: 3276 1063 1012 113 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2012 2034 2794 3161 3546 3563 5015 5101 Size: 40 22 760 367 385 17 1452 86 Cuts at: 5101 5113 5459 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 86 40 22 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2129 2382 513 Size: 552 939 125 253 3595 Fragments arranged by size: 3595 939 552 253 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2744 487 Size: 683 56 1518 3207 Fragments arranged by size: 3207 1518 683 56 BtsI GCAGTG_nn' Cuts at: 2164 2202 2212 2254 2980 3774 3794 2164 Size: 38 10 42 726 794 20 3834 Fragments arranged by size: 3834 794 726 42 38 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DraIII CAC_nnn'GTG Cuts at: 2733 2733 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2702 4471 809 Size: 507 1386 1769 1802 Fragments arranged by size: 1802 1769 1386 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2683 2911 3800 Size: 54 1425 50 275 18 853 228 889 Cuts at: 3800 5429 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 853 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3394 1353 Size: 2041 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2291 4051 4425 4571 387 Size: 1064 138 702 1760 374 146 1280 Fragments arranged by size: 1760 1280 1064 702 374 146 138 EcoICRI GAG'CTC Cuts at: 2273 2273 Size: 5464 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3527 4124 4911 5179 1239 Size: 393 1895 597 787 268 1524 Fragments arranged by size: 1895 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2106 2194 4611 1678 Size: 428 88 2417 2531 Fragments arranged by size: 2531 2417 428 88 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2004 2036 4105 4137 4179 Size: 32 135 32 432 32 2069 32 42 Cuts at: 4179 4211 1373 Size: 32 2626 Fragments arranged by size: 2626 2069 432 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KasI G'GCGC_C Cuts at: 2102 2102 Size: 5464 KpnI G_GTAC'C Cuts at: 2646 2789 2646 Size: 143 5321 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3642 Fragments arranged by size: 3642 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2334 2348 2498 2714 3422 3781 3940 Size: 648 1168 14 150 216 708 359 159 Cuts at: 3940 518 Size: 2042 Fragments arranged by size: 2042 1168 708 648 359 216 159 150 14 MspA1I CmG'CkG Cuts at: 1606 2412 2880 3557 4705 4950 1606 Size: 806 468 677 1148 245 2120 Fragments arranged by size: 2120 1148 806 677 468 245 NarI GG'CG_CC Cuts at: 2103 2103 Size: 5464 NcoI C'CATG_G Cuts at: 513 1065 2004 2129 2382 513 Size: 552 939 125 253 3595 Fragments arranged by size: 3595 939 552 253 125 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2822 4367 1132 Size: 97 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 97 PasI CC'CwG_GG Cuts at: 1348 2080 1348 Size: 732 4732 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2419 2451 2419 Size: 32 5432 PstI C_TGCA'G Cuts at: 830 2034 2068 2816 830 Size: 1204 34 748 3478 Fragments arranged by size: 3478 1204 748 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 SacI G_AGCT'C Cuts at: 2275 2275 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2030 2064 2812 3947 4628 4819 826 Size: 219 985 34 748 1135 681 191 1471 Fragments arranged by size: 1471 1135 985 748 681 219 191 34 SfoI GGC'GCC Cuts at: 2104 2104 Size: 5464 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2791 1872 Size: 919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2712 3568 4469 4731 820 Size: 197 926 769 856 901 262 1553 Fragments arranged by size: 1553 926 901 856 769 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 2288 1517 Size: 771 4693 StyI C'CwwG_G Cuts at: 513 1065 2004 2055 2129 2382 2903 513 Size: 552 939 51 74 253 521 3074 Fragments arranged by size: 3074 939 552 521 253 74 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2142 2185 3491 3508 3661 3846 4291 Size: 1255 562 43 1306 17 153 185 445 Cuts at: 4291 325 Size: 1498 Fragments arranged by size: 1498 1306 1255 562 445 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 2435 3710 Size: 274 80 33 51 1443 252 206 1275 Cuts at: 3710 5422 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1275 274 252 206 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 5424 5456 2616 Size: 32 2776 32 2624 Fragments arranged by size: 2776 2624 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2789 1870 Size: 919 4545 XmnI GAAnn'nnTTC Cuts at: 2297 3593 2297 Size: 1296 4168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI