Restriction map for
pFN21AE4503
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BbeI BbrPI BbsI BbvCI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsgI
BsiEI BsiHKAI BsiWI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MaeII
MamI MfeI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 355 TCCGGA T^CCGGA
AflIII 136 ACGCGT A^C[GA][CT]GT
BalI 618 TGGCCA TGG^CCA
BamHI 280 GGATCC G^GATCC
BcgI 1081 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 607 GTATCC GTATCC(6/5)
BcnI 1494 CCGGG CC^[GC]GG
BsaI 70 GGTCTC GGTCTC(1/5)
BseAI 355 TCCGGA T^CCGGA
BsmI 1099 GCATTC GAATGC(1/-1)
BspEI 355 TCCGGA T^CCGGA
BsrBI 1485 CCGCTC CCGCTC(-3/-3)
BsrFI 1279 ACCGGC [GA]^CCGG[CT]
BstEII 1363 GGTCACC G^GT[ACGT]ACC
BstPI 1363 GGTCACC G^GT[ACGT]ACC
Cfr10I 1279 ACCGGC [GA]^CCGG[CT]
DraIII 727 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 982 CCTGTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1363 GGTCACC G^GT[ACGT]ACC
EcoRV 1498 GATATC GAT^ATC
KspI 546 CCGCGG CCGC^GG
MluI 136 ACGCGT A^CGCGT
MluNI 618 TGGCCA TGG^CCA
MroI 355 TCCGGA T^CCGGA
MscI 618 TGGCCA TGG^CCA
MseI 317 TTAA T^TAA
NciI 1494 CCGGG CC^[GC]GG
NheI 1533 GCTAGC G^CTAGC
NspI 1373 GCATGT [GA]CATG^[CT]
PaeR7I 1184 CTCGAG C^TCGAG
PflMI 1104 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 546 CCGCGG CCGC^GG
SfaNI 382 GCATC GCATC(5/9)
SmlI 1184 CTCGAG C^T[CT][GA]AG
StuI 748 AGGCCT AGG^CCT
Tru9I 317 TTAA T^TAA
Van91I 1104 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1184 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 371 GTCTAC GT^[AC][GT]AC
862 GTAGAC
AccII 137 CGCG CG^CG
547 CGCG
BsmBI 8 CGTCTC CGTCTC(1/5)
693 CGTCTC
BstUI 137 CGCG CG^CG
547 CGCG
DsaI 546 CCGCGG C^C[GA][CT]GG
1148 CCACGG
EaeI 618 TGGCCA [CT]^GGCC[GA]
1091 CGGCCA
HgaI 626 GCGTC GACGC(5/10)
642 GCGTC
MvnI 137 CGCG CG^CG
547 CGCG
NlaIII 927 CATG CATG^
1374 CATG
PstI 305 CTGCAG CTGCA^G
675 CTGCAG
PvuII 397 CAGCTG CAG^CTG
1016 CAGCTG
SapI 581 GCTCTTC GCTCTTC(1/4)
1355 GCTCTTC
Tsp45I 179 GTGAC ^GT[GC]AC
1364 GTCAC
XcmI 501 CCAAGTAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
771 CCAGCACGGTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI ApaI AvaI
AvaII BanI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BsaWI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIV PleI
PpuMI Sau3AI Sau96I ScrFI SfcI
StyI TaqI TseI TspRI TthHB8I
XhoII