(Linear) (Six-base) MAPSORT of: KIEE4503.seq Check: 8261 from: 1 to: 1584 KIEE4503.orf With 182 enzymes: SgfI * March 22, 2012 18:13 .. AccI GT'mk_AC Cuts at: 0 372 863 1584 Size: 372 491 721 Fragments arranged by size: 721 491 372 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 1272 1315 1565 1584 Size: 177 1095 43 250 19 Fragments arranged by size: 1095 250 177 43 19 AflIII A'CryG_T Cuts at: 0 136 1584 Size: 136 1448 ApaI G_GGCC'C Cuts at: 0 689 1025 1408 1529 1584 Size: 689 336 383 121 55 Fragments arranged by size: 689 383 336 121 55 AvaI C'yCGr_G Cuts at: 0 11 420 441 1184 1584 Size: 11 409 21 743 400 Fragments arranged by size: 743 409 400 21 11 BamHI G'GATC_C Cuts at: 0 280 1584 Size: 280 1304 BanI G'GyrC_C Cuts at: 0 15 106 784 1276 1441 1584 Size: 15 91 678 492 165 143 Fragments arranged by size: 678 492 165 143 91 15 BanII G_rGCy'C Cuts at: 0 7 689 1025 1156 1261 1408 1529 1584 Size: 7 682 336 131 105 147 121 55 Fragments arranged by size: 682 336 147 131 121 105 55 7 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1070 1104 1584 Size: 1070 34 480 Fragments arranged by size: 1070 480 34 BciVI GTATCCnnnnn_n' Cuts at: 0 618 1584 Size: 618 966 BglI GCCn_nnn'nGGC Cuts at: 0 833 1412 1524 1584 Size: 833 579 112 60 Fragments arranged by size: 833 579 112 60 Bme1580I G_kGCm'C Cuts at: 0 18 44 689 789 1025 1408 1529 1584 Size: 18 26 645 100 236 383 121 55 Fragments arranged by size: 645 383 236 121 100 55 26 18 BmrI ACTGGGnnnn_n' Cuts at: 0 179 1409 1584 Size: 179 1230 175 Fragments arranged by size: 1230 179 175 BmtI G_CTAG'C Cuts at: 0 1537 1584 Size: 1537 47 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 168 195 246 258 812 980 1035 1161 Size: 168 27 51 12 554 168 55 126 Cuts at: 1161 1284 1443 1584 Size: 123 159 141 Fragments arranged by size: 554 168 168 159 141 126 123 55 51 27 12 BsaI GGTCTCn'nnnn_ Cuts at: 0 76 1584 Size: 76 1508 BsaWI w'CCGG_w Cuts at: 0 355 385 1219 1584 Size: 355 30 834 365 Fragments arranged by size: 834 365 355 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 193 223 1584 Size: 193 30 1361 Fragments arranged by size: 1361 193 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 64 342 891 1374 1584 Size: 64 278 549 483 210 Fragments arranged by size: 549 483 278 210 64 BseYI C'CCAG_C Cuts at: 0 395 399 566 718 1018 1069 1192 1529 Size: 395 4 167 152 300 51 123 337 Cuts at: 1529 1584 Size: 55 Fragments arranged by size: 395 337 300 167 152 123 55 51 4 BsmI GAATG_Cn' Cuts at: 0 1099 1584 Size: 1099 485 BsmBI CGTCTCn'nnnn_ Cuts at: 0 14 699 1584 Size: 14 685 885 Fragments arranged by size: 885 685 14 Bsp1286I G_dGCh'C Cuts at: 0 7 18 44 689 789 1025 1156 1261 Size: 7 11 26 645 100 236 131 105 Cuts at: 1261 1408 1529 1584 Size: 147 121 55 Fragments arranged by size: 645 236 147 131 121 105 100 55 26 11 7 BspEI T'CCGG_A Cuts at: 0 355 1584 Size: 355 1229 BsrBI CCG'CTC Cuts at: 0 1487 1584 Size: 1487 97 BsrDI GCAATG_nn' Cuts at: 0 287 825 1094 1584 Size: 287 538 269 490 Fragments arranged by size: 538 490 287 269 BsrFI r'CCGG_y Cuts at: 0 1279 1584 Size: 1279 305 BstEII G'GTnAC_C Cuts at: 0 1363 1584 Size: 1363 221 BstXI CCAn_nnnn'nTGG Cuts at: 0 601 1135 1315 1584 Size: 601 534 180 269 Fragments arranged by size: 601 534 269 180 BstYI r'GATC_y Cuts at: 0 280 310 358 1475 1584 Size: 280 30 48 1117 109 Fragments arranged by size: 1117 280 109 48 30 BtgI C'CryG_G Cuts at: 0 546 1148 1584 Size: 546 602 436 Fragments arranged by size: 602 546 436 BtsI GCAGTG_nn' Cuts at: 0 55 621 684 1251 1470 1584 Size: 55 566 63 567 219 114 Fragments arranged by size: 567 566 219 114 63 55 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1434 1469 1584 Size: 1434 35 115 Fragments arranged by size: 1434 115 35 DraIII CAC_nnn'GTG Cuts at: 0 732 1584 Size: 732 852 EaeI y'GGCC_r Cuts at: 0 618 1091 1584 Size: 618 473 493 Fragments arranged by size: 618 493 473 EarI CTCTTCn'nnn_ Cuts at: 0 588 594 1362 1584 Size: 588 6 768 222 Fragments arranged by size: 768 588 222 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 168 177 195 246 258 812 980 1035 Size: 168 9 18 51 12 554 168 55 Cuts at: 1035 1161 1272 1284 1315 1443 1565 1584 Size: 126 111 12 31 128 122 19 Fragments arranged by size: 554 168 168 128 126 122 111 55 51 31 19 18 12 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 986 1584 Size: 986 598 EcoO109I rG'GnC_Cy Cuts at: 0 86 129 685 805 1002 1022 1162 1404 Size: 86 43 556 120 197 20 140 242 Cuts at: 1404 1405 1525 1526 1584 Size: 1 120 1 58 Fragments arranged by size: 556 242 197 140 120 120 86 58 43 20 1 1 EcoRV GAT'ATC Cuts at: 0 1500 1584 Size: 1500 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 674 706 1584 Size: 674 32 878 Fragments arranged by size: 878 674 32 MluI A'CGCG_T Cuts at: 0 136 1584 Size: 136 1448 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 659 1584 Size: 659 925 MscI TGG'CCA Cuts at: 0 620 1584 Size: 620 964 MspA1I CmG'CkG Cuts at: 0 188 278 399 431 548 1018 1438 1584 Size: 188 90 121 32 117 470 420 146 Fragments arranged by size: 470 420 188 146 121 117 90 32 NheI G'CTAG_C Cuts at: 0 1533 1584 Size: 1533 51 NspI r_CATG'y Cuts at: 0 1377 1584 Size: 1377 207 PasI CC'CwG_GG Cuts at: 0 294 1584 Size: 294 1290 PflMI CCAn_nnn'nTGG Cuts at: 0 1110 1584 Size: 1110 474 PfoI T'CCnGG_A Cuts at: 0 1493 1584 Size: 1493 91 PpuMI rG'GwC_Cy Cuts at: 0 86 129 805 1002 1162 1584 Size: 86 43 676 197 160 422 Fragments arranged by size: 676 422 197 160 86 43 PspOMI G'GGCC_C Cuts at: 0 685 1021 1404 1525 1584 Size: 685 336 383 121 59 Fragments arranged by size: 685 383 336 121 59 PstI C_TGCA'G Cuts at: 0 309 679 1584 Size: 309 370 905 Fragments arranged by size: 905 370 309 PvuII CAG'CTG Cuts at: 0 399 1018 1584 Size: 399 619 566 Fragments arranged by size: 619 566 399 SacII CC_GC'GG Cuts at: 0 549 1584 Size: 549 1035 SanDI GG'GwC_CC Cuts at: 0 1002 1162 1584 Size: 1002 160 422 Fragments arranged by size: 1002 422 160 SapI GCTCTTCn'nnn_ Cuts at: 0 588 1362 1584 Size: 588 774 222 Fragments arranged by size: 774 588 222 SfcI C'TryA_G Cuts at: 0 201 305 648 675 813 860 933 1584 Size: 201 104 343 27 138 47 73 651 Fragments arranged by size: 651 343 201 138 104 73 47 27 SmlI C'TyrA_G Cuts at: 0 1184 1584 Size: 1184 400 StuI AGG'CCT Cuts at: 0 750 1584 Size: 750 834 StyI C'CwwG_G Cuts at: 0 89 1157 1560 1584 Size: 89 1068 403 24 Fragments arranged by size: 1068 403 89 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 25 883 1584 Size: 25 858 701 Fragments arranged by size: 858 701 25 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 15 47 1584 Size: 15 32 1537 Fragments arranged by size: 1537 32 15 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 508 778 1584 Size: 508 270 806 Fragments arranged by size: 806 508 270 XhoI C'TCGA_G Cuts at: 0 1184 1584 Size: 1184 400 Enzymes that do cut: AccI AcuI AflIII ApaI AvaI BamHI BanI BanII BcgI BciVI BglI Bme1580I BmrI BmtI BpmI BsaI BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI BtsI CspCI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV Hin4I MluI MmeI MscI MspA1I NheI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacII SanDI SapI SfcI SmlI StuI StyI TaqII TstI XcmI XhoI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BclI BfrBI BglII BlpI BmgBI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsgI BsiEI BsiHKAI BsiWI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MslI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XmaI XmnI ZraI