(Circular) (Six-base) MAPSORT of: pFN21AE1428.seq Check: 9805 from: 1 to: 5635 pFN21AE1428 5635 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2143 2143 Size: 5635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3698 5082 5350 1632 Size: 2066 1384 268 1917 Fragments arranged by size: 2066 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 2337 4534 5511 1203 Size: 1134 2197 977 1327 Fragments arranged by size: 2197 1327 1134 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 2062 4364 2062 Size: 2302 3333 AleI CACnn'nnGTG Cuts at: 1166 2577 1166 Size: 1411 4224 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2916 1184 Size: 1732 3903 AlwNI CAG_nnn'CTG Cuts at: 3378 4950 3378 Size: 1572 4063 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2522 2944 3170 3322 5431 1493 Size: 1029 422 226 152 2109 1697 Fragments arranged by size: 2109 1697 1029 422 226 152 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2165 2960 1870 Size: 73 222 795 4545 Fragments arranged by size: 4545 795 222 73 BamHI G'GATC_C Cuts at: 2536 2965 3332 2536 Size: 429 367 4839 Fragments arranged by size: 4839 429 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2457 2956 4411 618 Size: 325 217 627 670 499 1455 1842 Fragments arranged by size: 1842 1455 670 627 499 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2954 1391 Size: 781 782 4072 Fragments arranged by size: 4072 782 781 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BbvCI CC'TCA_GC Cuts at: 2363 2453 2363 Size: 90 5545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3826 3860 1903 Size: 34 1889 34 3678 Fragments arranged by size: 3678 1889 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2768 3527 4737 2768 Size: 759 1210 3666 Fragments arranged by size: 3666 1210 759 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2349 4246 136 Size: 107 122 71 631 605 677 1897 1525 Fragments arranged by size: 1897 1525 677 631 605 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 3052 3052 Size: 5635 Bme1580I G_kGCm'C Cuts at: 1790 3696 4852 1790 Size: 1906 1156 2573 Fragments arranged by size: 2573 1906 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5635 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2059 2167 2703 Size: 416 53 83 399 24 642 108 536 Cuts at: 2703 3366 4324 442 Size: 663 958 1753 Fragments arranged by size: 1753 958 663 642 536 416 399 108 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2260 2822 2260 Size: 562 5073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2393 2444 4295 1239 Size: 935 219 51 1851 2579 Fragments arranged by size: 2579 1851 935 219 51 Bpu10I CC'TnA_GC Cuts at: 2363 2453 2363 Size: 90 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2795 4298 882 Size: 617 1296 1503 2219 Fragments arranged by size: 2219 1503 1296 617 BsaAI yAC'GTr Cuts at: 493 1204 2340 3426 493 Size: 711 1136 1086 2702 Fragments arranged by size: 2702 1136 1086 711 BsaBI GATnn'nnATC Cuts at: 2672 5629 2672 Size: 2957 2678 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2354 1178 Size: 30 165 30 921 30 4459 Fragments arranged by size: 4459 921 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5635 BseYI C'CCAG_C Cuts at: 2070 2126 2294 2654 4838 5334 2070 Size: 56 168 360 2184 496 2371 Fragments arranged by size: 2371 2184 496 360 168 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2575 2806 1163 Size: 573 839 231 3992 Fragments arranged by size: 3992 839 573 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2409 3696 3781 4852 2190 Size: 219 1287 85 1071 2973 Fragments arranged by size: 2973 1287 1071 219 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2190 2409 2954 3696 3781 4852 Size: 399 382 18 219 545 742 85 1071 Cuts at: 4852 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 545 399 382 219 85 18 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2143 2990 844 Size: 1299 847 3489 Fragments arranged by size: 3489 1299 847 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4130 4304 65 Size: 1183 2882 174 1396 Fragments arranged by size: 2882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4279 4466 1700 Size: 110 2469 187 2869 Fragments arranged by size: 2869 2469 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5635 BssSI C'ACGA_G Cuts at: 3695 4707 3695 Size: 1012 4623 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2536 2965 3332 3717 3734 5186 5272 5284 Size: 564 429 367 385 17 1452 86 12 Cuts at: 5284 5630 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 564 429 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 2587 3151 3945 3965 2587 Size: 564 794 20 4257 Fragments arranged by size: 4257 794 564 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2350 2709 3082 Size: 54 1425 50 275 18 520 359 373 Cuts at: 3082 3971 5600 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 520 373 359 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2665 2904 3565 1353 Size: 1312 239 661 3423 Fragments arranged by size: 3423 1312 661 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2393 2444 3698 4295 5082 5350 Size: 393 542 219 51 1254 597 787 268 Cuts at: 5350 1239 Size: 1524 Fragments arranged by size: 1524 1254 787 597 542 393 268 219 51 EcoRI G'AATT_C Cuts at: 2944 3322 2944 Size: 378 5257 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 4782 1678 Size: 3104 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2430 2462 2749 Size: 32 135 32 476 32 350 32 287 Cuts at: 2749 2781 4276 4308 4350 4382 1373 Size: 32 1495 32 42 32 2626 Fragments arranged by size: 2626 1495 476 350 287 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2979 3225 669 Size: 988 281 1041 246 3079 Fragments arranged by size: 3079 1041 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2352 10 Size: 54 1425 343 520 3293 Fragments arranged by size: 3293 1425 520 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2577 2898 3593 3952 4111 518 Size: 648 1411 321 695 359 159 2042 Fragments arranged by size: 2042 1411 695 648 359 321 159 MspA1I CmG'CkG Cuts at: 1606 2102 2328 2466 3051 3728 4876 5121 1606 Size: 496 226 138 585 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 585 496 245 226 138 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4144 Fragments arranged by size: 4144 939 552 NdeI CA'TA_TG Cuts at: 387 2085 387 Size: 1698 3937 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2217 2993 4538 1132 Size: 97 988 776 1545 2229 Fragments arranged by size: 2229 1545 988 776 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5635 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 2253 1108 Size: 1145 4490 PfoI T'CCnGG_A Cuts at: 2190 2430 2190 Size: 240 5395 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PmlI CAC'GTG Cuts at: 2340 2340 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PspXI vC'TCGA_Gb Cuts at: 2165 2165 Size: 5635 PstI C_TGCA'G Cuts at: 830 2140 2747 2987 830 Size: 1310 607 240 3478 Fragments arranged by size: 3478 1310 607 240 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2102 2466 2102 Size: 364 5271 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SapI GCTCTTCn'nnn_ Cuts at: 2665 2904 2665 Size: 239 5396 SbfI CC_TGCA'GG Cuts at: 2987 2987 Size: 5635 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SfcI C'TryA_G Cuts at: 826 1045 2136 2299 2703 2724 2743 2983 4118 Size: 219 1091 163 404 21 19 240 1135 Cuts at: 4118 4799 4990 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1091 681 404 240 219 191 163 21 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2962 1872 Size: 1090 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 3739 4640 4902 820 Size: 197 926 222 1574 901 262 1553 Fragments arranged by size: 1574 1553 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5635 StyI C'CwwG_G Cuts at: 513 1065 2004 2075 3074 513 Size: 552 939 71 999 3074 Fragments arranged by size: 3074 999 939 552 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 3662 3679 3832 4017 4462 325 Size: 1255 717 1365 17 153 185 445 1498 Fragments arranged by size: 1498 1365 1255 717 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 2616 3881 Size: 274 80 33 51 1443 357 282 1265 Cuts at: 3881 5593 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1265 357 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 5595 5627 2324 Size: 32 3239 32 2332 Fragments arranged by size: 3239 2332 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2573 2573 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 2165 1943 Size: 222 5413 XmaI C'CCGG_G Cuts at: 1870 2960 1870 Size: 1090 4545 XmnI GAAnn'nnTTC Cuts at: 3764 3764 Size: 5635 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI