################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 34 GAATTC [GA]^AATT[CT] AflIII 321 ACACGT A^C[GA][CT]GT ApoI 34 GAATTC [GA]^AATT[CT] AvaI 199 CCCGAG C^[CT]CG[GA]G AvaII 113 GGTCC G^G[AT]CC BalI 218 TGGCCA TGG^CCA BanI 184 GGCACC G^G[CT][GA]CC BcgI 290 GCAACGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 99 GGGCAC G[AGT]GC[ACT]^C BsiEI 168 CGACCG CG[GA][CT]^CG BsmAI 398 GAGAC GTCTC(1/5) BsmBI 398 GAGACG CGTCTC(1/5) BsoBI 199 CCCGAG C^[CT]CG[GA]G Bsp1286I 99 GGGCAC G[AGT]GC[ACT]^C BspHI 326 TCATGA T^CATGA BspMI 24 GCAGGT ACCTGC(4/8) BstF5I 66 GGATG GGATG(2/0) BstYI 254 AGATCC [GA]^GATC[CT] Cac8I 92 GCGAGC GC[ACGT]^[ACGT]GC CfoI 305 GCGC GCG^C EcoRI 34 GAATTC G^AATTC FokI 66 GGATG GGATG(9/13) HaeII 304 AGCGCC [GA]GCGC^[CT] HhaI 305 GCGC GCG^C HinP1I 305 GCGC G^CGC KspI 383 CCGCGG CCGC^GG MaeII 323 ACGT A^CGT MboII 225 GAAGA GAAGA(8/7) MflI 254 AGATCC [GA]^GATC[CT] MluNI 218 TGGCCA TGG^CCA MscI 218 TGGCCA TGG^CCA MslI 102 CACGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PvuII 10 CAGCTG CAG^CTG RcaI 326 TCATGA T^CATGA SacII 383 CCGCGG CCGC^GG SfaNI 80 GCATC GCATC(5/9) Tsp509I 35 AATT ^AATT XcmI 86 CCACCCGCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 254 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 255 GATCC GGATC(4/5) 375 GATCC DdeI 17 CTGAG C^T[ACGT]AG 349 CTGAG EaeI 2 TGGCCG [CT]^GGCC[GA] 218 TGGCCA FauI 89 CCCGC CCCGC(4/6) 147 GCGGG HphI 408 GGTGA GGTGA(8/7) 83 TCACC MaeIII 28 GTCAC ^GT[ACGT]AC 353 GTGAC MspA1I 10 CAGCTG C[AC]G^C[GT]G 383 CCGCGG MwoI 92 GCGAGCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 296 GCTTCGTCAGC NlaIV 113 GGTCCC GG[ACGT]^[ACGT]CC 184 GGCACC StuI 44 AGGCCT AGG^CCT 263 AGGCCT TaqI 167 TCGA T^CGA 278 TCGA Tsp45I 28 GTCAC ^GT[GC]AC 353 GTGAC TthHB8I 167 TCGA T^CGA 278 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AccII AciI AluI BbvI BcnI BsaJI BseRI BsiYI BslI BsmFI BssKI BstNI BstUI Cfr13I DpnII DsaI EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboI MnlI MspI MvaI MvnI NciI NdeII NlaIII Sau3AI Sau96I ScrFI TseI