(Circular) (Six-base) MAPSORT of: pFN21AE6325.seq Check: 55 from: 1 to: 6010 pFN21AE6325 6010 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2315 2412 3038 4073 5457 5725 1632 Size: 683 97 626 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 683 626 268 97 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 4909 5886 1203 Size: 3706 977 1327 Fragments arranged by size: 3706 1327 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6010 AlwNI CAG_nnn'CTG Cuts at: 2773 3753 5325 2773 Size: 980 1572 3458 Fragments arranged by size: 3458 1572 980 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 2997 3092 3319 3545 3697 5806 1493 Size: 1504 95 227 226 152 2109 1697 Fragments arranged by size: 2109 1697 1504 227 226 152 95 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 3335 1870 Size: 73 1392 4545 Fragments arranged by size: 4545 1392 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3229 3262 3229 Size: 33 5977 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3126 3331 4786 618 Size: 325 217 627 1339 205 1455 1842 Fragments arranged by size: 1842 1455 1339 627 325 217 205 BanII G_rGCy'C Cuts at: 1391 2433 3329 1391 Size: 1042 896 4072 Fragments arranged by size: 4072 1042 896 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 928 Size: 971 560 4479 Fragments arranged by size: 4479 971 560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4201 4235 1903 Size: 34 2264 34 3678 Fragments arranged by size: 3678 2264 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 2571 1548 Size: 1023 4987 BfrBI ATG'CAT Cuts at: 2705 2705 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4621 136 Size: 107 122 71 631 605 2949 1525 Fragments arranged by size: 2949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2051 6005 2051 Size: 3954 2056 BlpI GC'TnA_GC Cuts at: 2432 3427 2432 Size: 995 5015 Bme1580I G_kGCm'C Cuts at: 1790 4071 5227 1790 Size: 2281 1156 2573 Fragments arranged by size: 2573 2281 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2220 2712 3162 Size: 416 53 83 399 24 803 492 450 Cuts at: 3162 3741 4699 442 Size: 579 958 1753 Fragments arranged by size: 1753 958 803 579 492 450 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2795 4670 1239 Size: 1556 1875 2579 Fragments arranged by size: 2579 1875 1556 Bpu10I CC'TnA_GC Cuts at: 2783 2783 Size: 6010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 4135 5000 5298 2195 Size: 1940 865 298 2907 Fragments arranged by size: 2907 1940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4673 882 Size: 617 3174 2219 Fragments arranged by size: 3174 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3817 4199 4834 275 Size: 53 83 186 936 2284 382 635 1451 Fragments arranged by size: 2284 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2499 4443 4841 5115 5262 5673 2499 Size: 1944 398 274 147 411 2836 Fragments arranged by size: 2836 1944 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3279 3309 1178 Size: 30 165 30 1876 30 3879 Fragments arranged by size: 3879 1876 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6010 BseYI C'CCAG_C Cuts at: 2437 5213 5709 2437 Size: 2776 496 2738 Fragments arranged by size: 2776 2738 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5437 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2433 4071 4156 5227 2433 Size: 1638 85 1071 3216 Fragments arranged by size: 3216 1638 1071 85 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2433 3329 4071 4156 5227 1391 Size: 399 643 896 742 85 1071 2174 Fragments arranged by size: 2174 1071 896 742 643 399 85 BspEI T'CCGG_A Cuts at: 2499 5673 2499 Size: 3174 2836 BspHI T'CATG_A Cuts at: 2536 3899 5629 2536 Size: 1363 1730 2917 Fragments arranged by size: 2917 1730 1363 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3365 844 Size: 2521 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3047 3181 4505 4679 65 Size: 1183 1799 134 1324 174 1396 Fragments arranged by size: 1799 1396 1324 1183 174 134 BsrFI r'CCGG_y Cuts at: 1700 1810 4654 4841 1700 Size: 110 2844 187 2869 Fragments arranged by size: 2869 2844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6010 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2051 2626 3340 3707 4092 4109 5561 5647 Size: 79 575 714 367 385 17 1452 86 Cuts at: 5647 5659 6005 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 714 575 385 367 346 86 79 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 3526 4320 4340 3526 Size: 794 20 5196 Fragments arranged by size: 5196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3084 3457 4346 Size: 54 1425 50 275 18 1254 373 889 Cuts at: 4346 5975 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1254 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 2106 3940 1353 Size: 720 33 1834 3423 Fragments arranged by size: 3423 1834 720 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2398 4597 4971 5117 387 Size: 1064 138 809 2199 374 146 1280 Fragments arranged by size: 2199 1280 1064 809 374 146 138 EcoICRI GAG'CTC Cuts at: 2431 2431 Size: 6010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2412 2795 3038 4073 4670 5457 Size: 393 683 97 383 243 1035 597 787 Cuts at: 5457 5725 1239 Size: 268 1524 Fragments arranged by size: 1524 1035 787 683 597 393 383 268 243 97 EcoNI CCTnn'n_nnAGG Cuts at: 3086 3086 Size: 6010 EcoO109I rG'GnC_Cy Cuts at: 2779 2779 Size: 6010 EcoRI G'AATT_C Cuts at: 3092 3319 3697 3092 Size: 227 378 5405 Fragments arranged by size: 5405 378 227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2135 2135 Size: 6010 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 5157 1678 Size: 3479 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2837 2869 3218 3250 4651 Size: 32 135 32 1265 32 349 32 1401 Cuts at: 4651 4683 4725 4757 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1401 1265 349 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3354 3600 669 Size: 988 281 1368 48 246 3079 Fragments arranged by size: 3079 1368 988 281 246 48 HindIII A'AGCT_T Cuts at: 748 2117 2297 748 Size: 1369 180 4461 Fragments arranged by size: 4461 1369 180 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2081 5124 5308 1305 Size: 776 3043 184 2007 Fragments arranged by size: 3043 2007 776 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3086 10 Size: 54 1425 343 1254 2934 Fragments arranged by size: 2934 1425 1254 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3012 3968 4327 4486 518 Size: 648 1846 956 359 159 2042 Fragments arranged by size: 2042 1846 956 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2390 2809 3426 4103 5251 5496 1606 Size: 784 419 617 677 1148 245 2120 Fragments arranged by size: 2120 1148 784 677 617 419 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NsiI A_TGCA'T Cuts at: 2707 2707 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 3368 4913 1132 Size: 97 2139 1545 2229 Fragments arranged by size: 2229 2139 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 4909 4909 Size: 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 2773 1108 Size: 1665 4345 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2779 2779 Size: 6010 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PstI C_TGCA'G Cuts at: 830 3362 830 Size: 2532 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2390 2809 2390 Size: 419 5591 SacI G_AGCT'C Cuts at: 2433 2433 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 4258 5970 4258 Size: 1712 4298 SfcI C'TryA_G Cuts at: 826 1045 3358 4493 5174 5365 826 Size: 219 2313 1135 681 191 1471 Fragments arranged by size: 2313 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2174 4114 5015 5277 820 Size: 197 926 231 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 231 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 2828 3934 5 Size: 47 2776 1106 2081 Fragments arranged by size: 2776 2081 1106 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2004 3087 3449 513 Size: 552 939 1083 362 3074 Fragments arranged by size: 3074 1083 939 552 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3114 4037 4054 4207 4392 4837 325 Size: 1255 1534 923 17 153 185 445 1498 Fragments arranged by size: 1534 1498 1255 923 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4256 5968 96 Size: 274 80 33 51 1443 2279 1712 138 Fragments arranged by size: 2279 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2212 2244 3279 3311 5970 6002 2212 Size: 32 1035 32 2659 32 2220 Fragments arranged by size: 2659 2220 1035 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 2743 3346 2743 Size: 603 5407 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2376 2376 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 2268 2495 4139 2268 Size: 227 1644 4139 Fragments arranged by size: 4139 1644 227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI