(Circular) (Six-base) MAPSORT of: pFN21AE3914.seq Check: 5700 from: 1 to: 5386 pFN21AE3914 5386 bp With 182 enzymes: SgfI * March 28, 2012 11:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2470 3196 278 Size: 53 83 186 936 934 726 2468 Fragments arranged by size: 2468 936 934 726 186 83 53 AccI GT'mk_AC Cuts at: 1937 2464 2729 1937 Size: 527 265 4594 Fragments arranged by size: 4594 527 265 Acc65I G'GTAC_C Cuts at: 2707 2707 Size: 5386 AclI AA'CG_TT Cuts at: 1563 3513 3886 1563 Size: 1950 373 3063 Fragments arranged by size: 3063 1950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3449 4833 5101 1632 Size: 1817 1384 268 1917 Fragments arranged by size: 1917 1817 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5189 AflIII A'CryG_T Cuts at: 1203 4285 5262 1203 Size: 3082 977 1327 Fragments arranged by size: 3082 1327 977 AgeI A'CCGG_T Cuts at: 4217 4217 Size: 5386 AhdI GACnn_n'nnGTC Cuts at: 4115 4115 Size: 5386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5386 AlwNI CAG_nnn'CTG Cuts at: 3129 4701 3129 Size: 1572 3814 ApaLI G'TGCA_C Cuts at: 3443 4599 3443 Size: 1156 4230 ApoI r'AATT_y Cuts at: 1493 2695 2921 3073 5182 1493 Size: 1202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1202 226 152 AseI AT'TA_AT Cuts at: 160 1032 2333 3940 160 Size: 872 1301 1607 1606 Fragments arranged by size: 1607 1606 1301 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 AvaI C'yCGr_G Cuts at: 1870 1943 2564 2711 1870 Size: 73 621 147 4545 Fragments arranged by size: 4545 621 147 73 BamHI G'GATC_C Cuts at: 2716 3083 2716 Size: 367 5019 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2707 4162 618 Size: 325 217 627 920 1455 1842 Fragments arranged by size: 1842 1455 920 627 325 217 BanII G_rGCy'C Cuts at: 1391 2705 1391 Size: 1314 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2454 2488 3577 3611 1903 Size: 34 517 34 1089 34 3678 Fragments arranged by size: 3678 1089 517 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3278 4488 3278 Size: 1210 4176 BclI T'GATC_A Cuts at: 1548 1548 Size: 5386 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3997 136 Size: 107 122 71 631 605 2325 1525 Fragments arranged by size: 2325 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5381 5381 Size: 5386 BlpI GC'TnA_GC Cuts at: 2803 2803 Size: 5386 Bme1580I G_kGCm'C Cuts at: 1790 3447 4603 1790 Size: 1657 1156 2573 Fragments arranged by size: 2573 1657 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5386 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3117 4075 442 Size: 416 53 83 399 24 1700 958 1753 Fragments arranged by size: 1753 1700 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5386 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 5386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 4046 1239 Size: 1277 1530 2579 Fragments arranged by size: 2579 1530 1277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2435 3511 4376 4674 2435 Size: 1076 865 298 3147 Fragments arranged by size: 3147 1076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2186 4049 882 Size: 617 687 1863 2219 Fragments arranged by size: 2219 1863 687 617 BsaAI yAC'GTr Cuts at: 493 1204 3177 493 Size: 711 1973 2702 Fragments arranged by size: 2702 1973 711 BsaBI GATnn'nnATC Cuts at: 5380 5380 Size: 5386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2467 3193 3575 4210 Size: 53 83 186 936 934 726 382 635 Cuts at: 4210 275 Size: 1451 Fragments arranged by size: 1451 936 934 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2640 3819 4217 4491 4638 5049 2640 Size: 1179 398 274 147 411 2977 Fragments arranged by size: 2977 1179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2544 2574 1178 Size: 30 165 30 1141 30 3990 Fragments arranged by size: 3990 1141 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 1186 Size: 1123 4263 BseYI C'CCAG_C Cuts at: 2015 2039 2243 4589 5085 2015 Size: 24 204 2346 496 2316 Fragments arranged by size: 2346 2316 496 204 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2525 2563 1163 Size: 573 789 38 3986 Fragments arranged by size: 3986 789 573 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2836 3597 4625 1540 Size: 275 186 835 761 1028 2301 Fragments arranged by size: 2301 1028 835 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2684 3447 3532 4603 2684 Size: 763 85 1071 3467 Fragments arranged by size: 3467 1071 763 85 BsmI GAATG_Cn' Cuts at: 2897 2990 2897 Size: 93 5293 BsmBI CGTCTCn'nnnn_ Cuts at: 2004 2004 Size: 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2684 2705 3447 3532 4603 1391 Size: 399 894 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 894 742 399 85 21 BspEI T'CCGG_A Cuts at: 5049 5049 Size: 5386 BspHI T'CATG_A Cuts at: 2619 3275 5005 2619 Size: 656 1730 3000 Fragments arranged by size: 3000 1730 656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2741 844 Size: 1897 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2150 3273 1394 Size: 429 327 1123 3507 Fragments arranged by size: 3507 1123 429 327 BsrDI GCAATG_nn' Cuts at: 65 1248 3881 4055 65 Size: 1183 2633 174 1396 Fragments arranged by size: 2633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4030 4217 1700 Size: 110 2220 187 2869 Fragments arranged by size: 2869 2220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5386 BssSI C'ACGA_G Cuts at: 3446 4458 3446 Size: 1012 4374 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5386 BstBI TT'CG_AA Cuts at: 4190 4190 Size: 5386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5386 BstYI r'GATC_y Cuts at: 1972 2351 2716 3083 3468 3485 4937 5023 5035 Size: 379 365 367 385 17 1452 86 12 Cuts at: 5035 5381 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 379 367 365 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2599 513 Size: 552 939 595 3300 Fragments arranged by size: 3300 939 595 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2540 487 Size: 683 56 1314 3333 Fragments arranged by size: 3333 1314 683 56 BtsI GCAGTG_nn' Cuts at: 2902 3696 3716 2902 Size: 794 20 4572 Fragments arranged by size: 4572 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5351 DraI TTT'AAA Cuts at: 2690 2690 Size: 5386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4393 809 Size: 507 3077 1802 Fragments arranged by size: 3077 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2402 2602 2833 Size: 54 1425 50 275 18 572 200 231 Cuts at: 2833 3722 5351 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 572 275 231 200 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2833 1537 Size: 275 1021 4090 Fragments arranged by size: 4090 1021 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2453 3316 1353 Size: 831 269 863 3423 Fragments arranged by size: 3423 863 831 269 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3973 4347 4493 387 Size: 1064 138 2384 374 146 1280 Fragments arranged by size: 2384 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2516 3449 4046 4833 5101 1239 Size: 393 884 933 597 787 268 1524 Fragments arranged by size: 1524 933 884 787 597 393 268 EcoRI G'AATT_C Cuts at: 2695 3073 2695 Size: 378 5008 FspI TGC'GCA Cuts at: 3892 3892 Size: 5386 HaeII r_GCGC'y Cuts at: 1678 2039 2656 4533 1678 Size: 361 617 1877 2531 Fragments arranged by size: 2531 1877 617 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2109 2357 2389 2452 Size: 32 135 32 505 32 248 32 63 Cuts at: 2452 2484 4027 4059 4101 4133 1373 Size: 32 1543 32 42 32 2626 Fragments arranged by size: 2626 1543 505 248 135 63 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2465 2730 2976 669 Size: 988 281 527 265 246 3079 Fragments arranged by size: 3079 988 527 281 265 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5386 HpaI GTT'AAC Cuts at: 2976 2976 Size: 5386 KpnI G_GTAC'C Cuts at: 2711 2711 Size: 5386 MfeI C'AATT_G Cuts at: 2985 2985 Size: 5386 MluI A'CGCG_T Cuts at: 5262 5262 Size: 5386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2285 4500 4684 1305 Size: 980 2215 184 2007 Fragments arranged by size: 2215 2007 980 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3564 Fragments arranged by size: 3564 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2624 3344 3703 3862 518 Size: 648 1458 720 359 159 2042 Fragments arranged by size: 2042 1458 720 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2802 3479 4627 4872 1606 Size: 1196 677 1148 245 2120 Fragments arranged by size: 2120 1196 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3895 Fragments arranged by size: 3895 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5386 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5386 NotI GC'GGCC_GC Cuts at: 2833 2833 Size: 5386 NspI r_CATG'y Cuts at: 1132 1229 2744 4289 1132 Size: 97 1515 1545 2229 Fragments arranged by size: 2229 1545 1515 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5386 PciI A'CATG_T Cuts at: 4285 4285 Size: 5386 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5386 PmeI GTTT'AAAC Cuts at: 2690 2690 Size: 5386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 4968 5000 3455 Size: 32 1481 32 3841 Fragments arranged by size: 3841 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5386 PsiI TTA'TAA Cuts at: 2956 2956 Size: 5386 PstI C_TGCA'G Cuts at: 830 2738 830 Size: 1908 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5386 RsrII CG'GwC_CG Cuts at: 2430 2430 Size: 5386 SalI G'TCGA_C Cuts at: 1936 2463 2728 1936 Size: 527 265 4594 Fragments arranged by size: 4594 527 265 SbfI CC_TGCA'GG Cuts at: 2738 2738 Size: 5386 ScaI AGT'ACT Cuts at: 3634 5346 3634 Size: 1712 3674 SfcI C'TryA_G Cuts at: 826 1045 2734 3869 4550 4741 826 Size: 219 1689 1135 681 191 1471 Fragments arranged by size: 1689 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5386 SmaI CCC'GGG Cuts at: 1872 2713 1872 Size: 841 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2414 3490 4391 4653 820 Size: 197 926 471 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 471 262 197 SnaBI TAC'GTA Cuts at: 493 3177 493 Size: 2684 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5386 SphI G_CATG'C Cuts at: 1132 2744 1132 Size: 1612 3774 SspI AAT'ATT Cuts at: 5 52 3310 5 Size: 47 3258 2081 Fragments arranged by size: 3258 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5386 StyI C'CwwG_G Cuts at: 513 1065 2004 2825 513 Size: 552 939 821 3074 Fragments arranged by size: 3074 939 821 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3413 3430 3583 3768 4213 325 Size: 1255 1833 17 153 185 445 1498 Fragments arranged by size: 1833 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2156 3632 5344 Size: 274 80 33 51 1443 179 1476 1712 Cuts at: 5344 96 Size: 138 Fragments arranged by size: 1712 1476 1443 274 179 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5346 5378 5346 Size: 32 5354 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5386 XbaI T'CTAG_A Cuts at: 2722 2722 Size: 5386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2597 2623 2497 Size: 100 26 5260 Fragments arranged by size: 5260 100 26 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5386 XmaI C'CCGG_G Cuts at: 1870 2711 1870 Size: 841 4545 XmnI GAAnn'nnTTC Cuts at: 3515 3515 Size: 5386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2468 3194 276 Size: 53 83 186 936 934 726 2468 Fragments arranged by size: 2468 936 934 726 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI