################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflIII AgeI AhdI AocI ApaI AscI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BclI BfaI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MaeI MamI MluI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 639 GTCTAC GT^[AC][GT]AC AflII 1903 CTTAAG C^TTAAG Alw44I 1154 GTGCAC G^TGCAC Aor51HI 1505 AGCGCT AGC^GCT ApaLI 1154 GTGCAC G^TGCAC AseI 1069 ATTAAT AT^TAAT AsnI 1069 ATTAAT AT^TAAT AvaI 810 CCCGGG C^[CT]CG[GA]G BalI 1718 TGGCCA TGG^CCA BamHI 1898 GGATCC G^GATCC BanII 1184 GAGCTC G[GA]GC[CT]^C BciVI 230 GGATAC GTATCC(6/5) BfrI 1903 CTTAAG C^TTAAG BsiYI 948 CCTGAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 948 CCTGAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 1401 GGGAC GGGAC(10/14) BsoBI 810 CCCGGG C^[CT]CG[GA]G BspMI 1814 ACCTGC ACCTGC(4/8) BstXI 1560 CCAAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1506 GCGC GCG^C EaeI 1718 TGGCCA [CT]^GGCC[GA] Eco47III 1505 AGCGCT AGC^GCT EcoRV 1828 GATATC GAT^ATC HaeII 1505 AGCGCT [GA]GCGC^[CT] HhaI 1506 GCGC GCG^C HinP1I 1506 GCGC G^CGC HincII 2091 GTCAAC GT[CT]^[GA]AC HindII 2091 GTCAAC GT[CT]^[GA]AC HindIII 1106 AAGCTT A^AGCTT MaeII 1998 ACGT A^CGT MfeI 346 CAATTG C^AATTG MluNI 1718 TGGCCA TGG^CCA MscI 1718 TGGCCA TGG^CCA MunI 346 CAATTG C^AATTG PshBI 1069 ATTAAT AT^TAAT PstI 389 CTGCAG CTGCA^G SacI 1184 GAGCTC GAGCT^C SmaI 810 CCCGGG CCC^GGG XmaCI 810 CCCGGG C^CCGGG XmaI 810 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccIII 6 TCCGGA T^CCGGA 1895 TCCGGA AlwNI 723 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 943 CAGTACCTG Asp700I 416 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 427 GAAGTCTTTC AspHI 1154 GTGCAC G[AT]GC[AT]^C 1184 GAGCTC BcnI 810 CCCGG CC^[GC]GG 811 CCGGG BglII 715 AGATCT A^GATCT 1605 AGATCT BmyI 1154 GTGCAC G[AGT]GC[ACT]^C 1184 GAGCTC BsaWI 6 TCCGGA [AT]^CCGG[AT] 1895 TCCGGA BseAI 6 TCCGGA T^CCGGA 1895 TCCGGA BsiHKAI 1154 GTGCAC G[AT]GC[AT]^C 1184 GAGCTC BsmI 585 GAATGC GAATGC(1/-1) 1636 GCATTC Bsp1286I 1154 GTGCAC G[AGT]GC[ACT]^C 1184 GAGCTC BspEI 6 TCCGGA T^CCGGA 1895 TCCGGA BstNI 108 CCTGG CC^[AT]GG 551 CCTGG Cac8I 1429 GCAAGC GC[ACGT]^[ACGT]GC 1756 GCTGGC DraII 815 GGGTCCT [GA]G^G[ACGT]CC[CT] 1868 AGGACCT EcoO109I 815 GGGTCCT [GA]G^G[ACGT]CC[CT] 1868 AGGACCT EcoRII 108 CCTGG ^CC[AT]GG 551 CCTGG EcoT22I 1057 ATGCAT ATGCA^T 1244 ATGCAT HaeIII 1719 GGCC GG^CC 1990 GGCC MroI 6 TCCGGA T^CCGGA 1895 TCCGGA MslI 1290 CATTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1666 CATTTGGATG MspA1I 1041 CAGCTG C[AC]G^C[GT]G 1690 CAGCTG MvaI 108 CCTGG CC^[AT]GG 551 CCTGG NciI 810 CCCGG CC^[GC]GG 811 CCGGG NsiI 1057 ATGCAT ATGCA^T 1244 ATGCAT NspI 136 ACATGC [GA]CATG^[CT] 1076 ACATGC PpuMI 815 GGGTCCT [GA]G^G[AT]CC[CT] 1868 AGGACCT PvuII 1041 CAGCTG CAG^CTG 1690 CAGCTG SspI 486 AATATT AAT^ATT 1924 AATATT XmnI 416 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 427 GAAGTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvI BpuAI BsaJI BsgI BsmAI BsrDI BsrI BssKI BstF5I BstYI Cfr13I DdeI DpnII DraI EcoT14I Fnu4HI FokI HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MspI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII