(Circular) (Six-base) MAPSORT of: pFN21AE3919.seq Check: 6228 from: 1 to: 6826 pFN21AE3919 6826 bp With 182 enzymes: SgfI * March 28, 2012 11:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4636 278 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 4169 1937 Size: 708 1524 4594 Fragments arranged by size: 4594 1524 708 Acc65I G'GTAC_C Cuts at: 4147 4147 Size: 6826 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2451 3013 3863 4889 6273 6541 1632 Size: 819 562 850 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 850 819 562 268 AfeI AGC'GCT Cuts at: 3512 3512 Size: 6826 AflII C'TTAA_G Cuts at: 820 1017 3908 820 Size: 197 2891 3738 Fragments arranged by size: 3738 2891 197 AflIII A'CryG_T Cuts at: 1203 5725 6702 1203 Size: 4522 977 1327 Fragments arranged by size: 4522 1327 977 AgeI A'CCGG_T Cuts at: 5657 5657 Size: 6826 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6826 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2158 3064 3488 1184 Size: 974 906 424 4522 Fragments arranged by size: 4522 974 906 424 AlwNI CAG_nnn'CTG Cuts at: 2733 2953 4569 6141 2733 Size: 220 1616 1572 3418 Fragments arranged by size: 3418 1616 1572 220 ApaLI G'TGCA_C Cuts at: 3159 4883 6039 3159 Size: 1724 1156 3946 Fragments arranged by size: 3946 1724 1156 ApoI r'AATT_y Cuts at: 1493 2683 3260 3373 3457 4135 4361 4513 6622 Size: 1190 577 113 84 678 226 152 2109 Cuts at: 6622 1493 Size: 1697 Fragments arranged by size: 2109 1697 1190 678 577 226 152 113 84 AseI AT'TA_AT Cuts at: 160 1032 3075 5380 160 Size: 872 2043 2305 1606 Fragments arranged by size: 2305 2043 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 2815 4151 1870 Size: 73 872 1336 4545 Fragments arranged by size: 4545 1336 872 73 BamHI G'GATC_C Cuts at: 3903 4156 4523 3903 Size: 253 367 6206 Fragments arranged by size: 6206 367 253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4147 5602 618 Size: 325 217 627 2360 1455 1842 Fragments arranged by size: 2360 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3193 4145 1391 Size: 1802 952 4072 Fragments arranged by size: 4072 1802 952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 2690 4119 928 Size: 971 306 485 1429 3635 Fragments arranged by size: 3635 1429 971 485 306 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5017 5051 1903 Size: 34 3080 34 3678 Fragments arranged by size: 3678 3080 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2229 4718 5928 2229 Size: 2489 1210 3127 Fragments arranged by size: 3127 2489 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BfrBI ATG'CAT Cuts at: 3064 3251 3064 Size: 187 6639 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5437 136 Size: 107 122 71 631 605 3765 1525 Fragments arranged by size: 3765 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2720 3610 6821 2720 Size: 890 3211 2725 Fragments arranged by size: 3211 2725 890 BlpI GC'TnA_GC Cuts at: 4243 4243 Size: 6826 Bme1580I G_kGCm'C Cuts at: 1790 3163 4887 6043 1790 Size: 1373 1724 1156 2573 Fragments arranged by size: 2573 1724 1373 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6826 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4557 5515 442 Size: 416 53 83 399 24 3140 958 1753 Fragments arranged by size: 3140 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6826 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5486 1239 Size: 4247 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3553 3890 4951 5816 6114 3553 Size: 337 1061 865 298 4265 Fragments arranged by size: 4265 1061 865 337 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5489 882 Size: 617 3990 2219 Fragments arranged by size: 3990 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4617 493 Size: 711 3413 2702 Fragments arranged by size: 3413 2702 711 BsaBI GATnn'nnATC Cuts at: 6820 6820 Size: 6826 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4633 5015 5650 275 Size: 53 83 186 936 3100 382 635 1451 Fragments arranged by size: 3100 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2011 3900 5259 5657 5931 6078 6489 2011 Size: 1889 1359 398 274 147 411 2348 Fragments arranged by size: 2348 1889 1359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6601 Fragments arranged by size: 6601 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6826 BseYI C'CCAG_C Cuts at: 3693 6029 6525 3693 Size: 2336 496 3994 Fragments arranged by size: 3994 2336 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2989 3034 3806 3907 1163 Size: 573 1253 45 772 101 4082 Fragments arranged by size: 4082 1253 772 573 101 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4276 5037 6065 1540 Size: 275 186 2275 761 1028 2301 Fragments arranged by size: 2301 2275 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3163 3193 4887 4972 6043 3163 Size: 30 1694 85 1071 3946 Fragments arranged by size: 3946 1694 1071 85 30 BsmI GAATG_Cn' Cuts at: 2596 3641 4337 4430 2596 Size: 1045 696 93 4992 Fragments arranged by size: 4992 1045 696 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3163 3193 4145 4887 4972 6043 1391 Size: 399 1373 30 952 742 85 1071 2174 Fragments arranged by size: 2174 1373 1071 952 742 399 85 30 BspEI T'CCGG_A Cuts at: 2011 3900 6489 2011 Size: 1889 2589 2348 Fragments arranged by size: 2589 2348 1889 BspHI T'CATG_A Cuts at: 4715 6445 4715 Size: 1730 5096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3828 4181 844 Size: 2984 353 3489 Fragments arranged by size: 3489 2984 353 BsrBI CCG'CTC Cuts at: 1394 1823 4713 1394 Size: 429 2890 3507 Fragments arranged by size: 3507 2890 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3036 3756 3795 5321 5495 65 Size: 1183 1788 720 39 1526 174 1396 Fragments arranged by size: 1788 1526 1396 1183 720 174 39 BsrFI r'CCGG_y Cuts at: 1700 1810 5470 5657 1700 Size: 110 3660 187 2869 Fragments arranged by size: 3660 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6826 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6826 BssSI C'ACGA_G Cuts at: 4886 5898 4886 Size: 1012 5814 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6826 BstBI TT'CG_AA Cuts at: 5630 5630 Size: 6826 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6826 BstXI CCAn_nnnn'nTGG Cuts at: 3572 3572 Size: 6826 BstYI r'GATC_y Cuts at: 1972 2720 3216 3338 3610 3903 4156 4523 4908 Size: 748 496 122 272 293 253 367 385 Cuts at: 4908 4925 6377 6463 6475 6821 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 748 496 385 367 346 293 272 253 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6274 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3126 487 Size: 683 56 1900 4187 Fragments arranged by size: 4187 1900 683 56 BtsI GCAGTG_nn' Cuts at: 2142 4114 4342 5136 5156 2142 Size: 1972 228 794 20 3812 Fragments arranged by size: 3812 1972 794 228 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6791 DraI TTT'AAA Cuts at: 2520 2667 3525 4130 2520 Size: 147 858 605 5216 Fragments arranged by size: 5216 858 605 147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5833 809 Size: 507 4517 1802 Fragments arranged by size: 4517 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3723 4273 5162 Size: 54 1425 50 275 18 1893 550 889 Cuts at: 5162 6791 8 Size: 1629 43 Fragments arranged by size: 1893 1629 1425 889 550 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4273 1537 Size: 275 2461 4090 Fragments arranged by size: 4090 2461 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4756 1353 Size: 3403 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2302 5413 5787 5933 387 Size: 1064 138 713 3111 374 146 1280 Fragments arranged by size: 3111 1280 1064 713 374 146 138 EcoICRI GAG'CTC Cuts at: 3191 3191 Size: 6826 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2451 3013 3863 4889 5486 6273 6541 Size: 393 819 562 850 1026 597 787 268 Cuts at: 6541 1239 Size: 1524 Fragments arranged by size: 1524 1026 850 819 787 597 562 393 268 EcoO109I rG'GnC_Cy Cuts at: 2821 3874 2821 Size: 1053 5773 EcoRI G'AATT_C Cuts at: 4135 4513 4135 Size: 378 6448 EcoRV GAT'ATC Cuts at: 3835 3835 Size: 6826 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2231 2456 2231 Size: 225 6601 FspI TGC'GCA Cuts at: 5332 5332 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 3514 5973 1678 Size: 1836 2459 2531 Fragments arranged by size: 2531 2459 1836 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 3785 3817 5467 Size: 32 135 32 1019 32 1162 32 1650 Cuts at: 5467 5499 5541 5573 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1650 1162 1019 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4098 4170 4416 669 Size: 988 281 2160 72 246 3079 Fragments arranged by size: 3079 2160 988 281 246 72 HindIII A'AGCT_T Cuts at: 748 3111 748 Size: 2363 4463 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KpnI G_GTAC'C Cuts at: 4151 4151 Size: 6826 MfeI C'AATT_G Cuts at: 2351 4425 2351 Size: 2074 4752 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2728 5940 6124 1305 Size: 1423 3212 184 2007 Fragments arranged by size: 3212 2007 1423 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3725 10 Size: 54 1425 343 1893 3111 Fragments arranged by size: 3111 1893 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3299 3675 4784 5143 5302 518 Size: 648 2133 376 1109 359 159 2042 Fragments arranged by size: 2133 2042 1109 648 376 359 159 MspA1I CmG'CkG Cuts at: 1606 3048 3697 4124 4242 4919 6067 6312 1606 Size: 1442 649 427 118 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 649 427 245 118 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6274 NdeI CA'TA_TG Cuts at: 387 387 Size: 6826 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6826 NotI GC'GGCC_GC Cuts at: 4273 4273 Size: 6826 NsiI A_TGCA'T Cuts at: 3066 3253 3066 Size: 187 6639 NspI r_CATG'y Cuts at: 1132 1229 2145 3085 4184 5729 1132 Size: 97 916 940 1099 1545 2229 Fragments arranged by size: 2229 1545 1099 940 916 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6826 PciI A'CATG_T Cuts at: 5725 5725 Size: 6826 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6826 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4895 4927 6408 6440 4895 Size: 32 1481 32 5281 Fragments arranged by size: 5281 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2821 3874 2821 Size: 1053 5773 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 4396 4396 Size: 6826 PstI C_TGCA'G Cuts at: 830 2398 4178 830 Size: 1568 1780 3478 Fragments arranged by size: 3478 1780 1568 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 PvuII CAG'CTG Cuts at: 3048 3697 3048 Size: 649 6177 SacI G_AGCT'C Cuts at: 3193 3193 Size: 6826 SalI G'TCGA_C Cuts at: 1936 4168 1936 Size: 2232 4594 SbfI CC_TGCA'GG Cuts at: 4178 4178 Size: 6826 ScaI AGT'ACT Cuts at: 5074 6786 5074 Size: 1712 5114 SfcI C'TryA_G Cuts at: 826 1045 2032 2394 2734 4174 5309 5990 6181 Size: 219 987 362 340 1440 1135 681 191 Cuts at: 6181 826 Size: 1471 Fragments arranged by size: 1471 1440 1135 987 681 362 340 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 2817 4153 1872 Size: 945 1336 4545 Fragments arranged by size: 4545 1336 945 SmlI C'TyrA_G Cuts at: 820 1017 1943 3532 3869 3908 4930 5831 6093 Size: 197 926 1589 337 39 1022 901 262 Cuts at: 6093 820 Size: 1553 Fragments arranged by size: 1589 1553 1022 926 901 337 262 197 39 SnaBI TAC'GTA Cuts at: 493 4617 493 Size: 4124 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6826 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SspI AAT'ATT Cuts at: 5 52 2493 3931 4750 5 Size: 47 2441 1438 819 2081 Fragments arranged by size: 2441 2081 1438 819 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6826 StyI C'CwwG_G Cuts at: 513 1065 3022 3565 3597 4265 513 Size: 552 1957 543 32 668 3074 Fragments arranged by size: 3074 1957 668 552 543 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3023 4853 4870 5023 5208 5653 325 Size: 1255 1443 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1443 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3179 5072 6784 Size: 274 80 33 51 1443 1202 1893 1712 Cuts at: 6784 96 Size: 138 Fragments arranged by size: 1893 1712 1443 1202 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6786 6818 6786 Size: 32 6794 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6826 XbaI T'CTAG_A Cuts at: 4162 4162 Size: 6826 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6826 XmaI C'CCGG_G Cuts at: 1870 2815 4151 1870 Size: 945 1336 4545 Fragments arranged by size: 4545 1336 945 XmnI GAAnn'nnTTC Cuts at: 2425 2436 4955 2425 Size: 11 2519 4296 Fragments arranged by size: 4296 2519 11 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4634 276 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI