(Linear) (Six-base) MAPSORT of: KIEE3919.seq Check: 3853 from: 1 to: 2121 KIEE3919.orf With 182 enzymes: SgfI * March 22, 2012 16:50 .. AccI GT'mk_AC Cuts at: 0 640 2121 Size: 640 1481 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1008 1858 2121 Size: 446 562 850 263 Fragments arranged by size: 850 562 446 263 AfeI AGC'GCT Cuts at: 0 1507 2121 Size: 1507 614 AflII C'TTAA_G Cuts at: 0 1903 2121 Size: 1903 218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 153 1059 1483 2121 Size: 153 906 424 638 Fragments arranged by size: 906 638 424 153 AlwNI CAG_nnn'CTG Cuts at: 0 728 948 2121 Size: 728 220 1173 Fragments arranged by size: 1173 728 220 ApaLI G'TGCA_C Cuts at: 0 1154 2121 Size: 1154 967 ApoI r'AATT_y Cuts at: 0 678 1255 1368 1452 2121 Size: 678 577 113 84 669 Fragments arranged by size: 678 669 577 113 84 AseI AT'TA_AT Cuts at: 0 1070 2121 Size: 1070 1051 AvaI C'yCGr_G Cuts at: 0 810 2121 Size: 810 1311 BamHI G'GATC_C Cuts at: 0 1898 2121 Size: 1898 223 BanII G_rGCy'C Cuts at: 0 1188 2121 Size: 1188 933 BbsI GAAGACnn'nnnn_ Cuts at: 0 200 685 2114 2121 Size: 200 485 1429 7 Fragments arranged by size: 1429 485 200 7 BciVI GTATCCnnnnn_n' Cuts at: 0 224 2121 Size: 224 1897 BfrBI ATG'CAT Cuts at: 0 1059 1246 2121 Size: 1059 187 875 Fragments arranged by size: 1059 875 187 BglII A'GATC_T Cuts at: 0 715 1605 2121 Size: 715 890 516 Fragments arranged by size: 890 715 516 Bme1580I G_kGCm'C Cuts at: 0 1158 2121 Size: 1158 963 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1548 1885 2121 Size: 1548 337 236 Fragments arranged by size: 1548 337 236 BsaWI w'CCGG_w Cuts at: 0 6 1895 2121 Size: 6 1889 226 Fragments arranged by size: 1889 226 6 BseYI C'CCAG_C Cuts at: 0 1688 2121 Size: 1688 433 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 984 1029 1801 1902 2121 Size: 984 45 772 101 219 Fragments arranged by size: 984 772 219 101 45 BsiHKAI G_wGCw'C Cuts at: 0 1158 1188 2121 Size: 1158 30 933 Fragments arranged by size: 1158 933 30 BsmI GAATG_Cn' Cuts at: 0 591 1636 2121 Size: 591 1045 485 Fragments arranged by size: 1045 591 485 Bsp1286I G_dGCh'C Cuts at: 0 1158 1188 2121 Size: 1158 30 933 Fragments arranged by size: 1158 933 30 BspEI T'CCGG_A Cuts at: 0 6 1895 2121 Size: 6 1889 226 Fragments arranged by size: 1889 226 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1823 2121 Size: 1823 298 BsrDI GCAATG_nn' Cuts at: 0 1031 1751 1790 2121 Size: 1031 720 39 331 Fragments arranged by size: 1031 720 331 39 BstXI CCAn_nnnn'nTGG Cuts at: 0 1567 2121 Size: 1567 554 BstYI r'GATC_y Cuts at: 0 715 1211 1333 1605 1898 2121 Size: 715 496 122 272 293 223 Fragments arranged by size: 715 496 293 272 223 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1121 2121 Size: 1121 1000 BtsI GCAGTG_nn' Cuts at: 0 137 2109 2121 Size: 137 1972 12 Fragments arranged by size: 1972 137 12 DraI TTT'AAA Cuts at: 0 515 662 1520 2121 Size: 515 147 858 601 Fragments arranged by size: 858 601 515 147 EaeI y'GGCC_r Cuts at: 0 1718 2121 Size: 1718 403 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 297 2121 Size: 297 1824 EcoICRI GAG'CTC Cuts at: 0 1186 2121 Size: 1186 935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 446 1008 1858 2121 Size: 446 562 850 263 Fragments arranged by size: 850 562 446 263 EcoO109I rG'GnC_Cy Cuts at: 0 816 1869 2121 Size: 816 1053 252 Fragments arranged by size: 1053 816 252 EcoRV GAT'ATC Cuts at: 0 1830 2121 Size: 1830 291 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 226 451 2121 Size: 226 225 1670 Fragments arranged by size: 1670 226 225 HaeII r_GCGC'y Cuts at: 0 1509 2121 Size: 1509 612 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 586 618 1780 1812 2121 Size: 586 32 1162 32 309 Fragments arranged by size: 1162 586 309 32 32 HincII GTy'rAC Cuts at: 0 2093 2121 Size: 2093 28 HindIII A'AGCT_T Cuts at: 0 1106 2121 Size: 1106 1015 MfeI C'AATT_G Cuts at: 0 346 2121 Size: 346 1775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 723 2121 Size: 723 1398 MscI TGG'CCA Cuts at: 0 1720 2121 Size: 1720 401 MslI CAynn'nnrTG Cuts at: 0 1294 1670 2121 Size: 1294 376 451 Fragments arranged by size: 1294 451 376 MspA1I CmG'CkG Cuts at: 0 1043 1692 2121 Size: 1043 649 429 Fragments arranged by size: 1043 649 429 NsiI A_TGCA'T Cuts at: 0 1061 1248 2121 Size: 1061 187 873 Fragments arranged by size: 1061 873 187 NspI r_CATG'y Cuts at: 0 140 1080 2121 Size: 140 940 1041 Fragments arranged by size: 1041 940 140 PpuMI rG'GwC_Cy Cuts at: 0 816 1869 2121 Size: 816 1053 252 Fragments arranged by size: 1053 816 252 PstI C_TGCA'G Cuts at: 0 393 2121 Size: 393 1728 PvuII CAG'CTG Cuts at: 0 1043 1692 2121 Size: 1043 649 429 Fragments arranged by size: 1043 649 429 SacI G_AGCT'C Cuts at: 0 1188 2121 Size: 1188 933 SfcI C'TryA_G Cuts at: 0 27 389 729 2121 Size: 27 362 340 1392 Fragments arranged by size: 1392 362 340 27 SmaI CCC'GGG Cuts at: 0 812 2121 Size: 812 1309 SmlI C'TyrA_G Cuts at: 0 1527 1864 1903 2121 Size: 1527 337 39 218 Fragments arranged by size: 1527 337 218 39 SspI AAT'ATT Cuts at: 0 488 1926 2121 Size: 488 1438 195 Fragments arranged by size: 1438 488 195 StyI C'CwwG_G Cuts at: 0 1017 1560 1592 2121 Size: 1017 543 32 529 Fragments arranged by size: 1017 543 529 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1018 2121 Size: 1018 1103 TatI w'GTAC_w Cuts at: 0 1174 2121 Size: 1174 947 XmaI C'CCGG_G Cuts at: 0 810 2121 Size: 810 1311 XmnI GAAnn'nnTTC Cuts at: 0 420 431 2121 Size: 420 11 1690 Fragments arranged by size: 1690 420 11 Enzymes that do cut: AccI AcuI AfeI AflII AlfI AlwNI ApaLI ApoI AseI AvaI BamHI BanII BbsI BciVI BfrBI BglII Bme1580I BpuEI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstXI BstYI BtgZI BtsI DraI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NsiI NspI PpuMI PstI PvuII SacI SfcI SmaI SmlI SspI StyI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AgeI AhdI AleI AloI ApaI AscI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI BglI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII DrdI EagI EarI EcoNI EcoRI FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI ZraI