################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AclI 1287 AACGTT AA^CGTT AflIII 1412 ACGTGT A^C[GA][CT]GT AspHI 430 GAGCAC G[AT]GC[AT]^C BanI 1777 GGCACC G^G[CT][GA]CC BanII 760 GGGCTC G[GA]GC[CT]^C BglI 1307 GCCTCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 1333 GAGACC GGTCTC(1/5) BsgI 460 GTGCAG GTGCAG(16/14) BsiHKAI 430 GAGCAC G[AT]GC[AT]^C BsiWI 1511 CGTACG C^GTACG BsmFI 2198 GTCCC GGGAC(10/14) BsrFI 1892 GCCGGT [GA]^CCGG[CT] BstAPI 594 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1591 GGTCACC G^GT[ACGT]ACC BstPI 1591 GGTCACC G^GT[ACGT]ACC BstXI 1322 CCATAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1892 GCCGGT [GA]^CCGG[CT] EcoO65I 1591 GGTCACC G^GT[ACGT]ACC EcoT22I 295 ATGCAT ATGCA^T HapII 1893 CCGG C^CGG HindIII 1561 AAGCTT A^AGCTT HpaII 1893 CCGG C^CGG MspI 1893 CCGG C^CGG NsiI 295 ATGCAT ATGCA^T PpuMI 263 AGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 1287 AACGTT AA^CGTT SapI 697 GAAGAGC GCTCTTC(1/4) SexAI 1629 ACCTGGT A^CC[AT]GGT XbaI 378 TCTAGA T^CTAGA XcmI 1839 CCATTCTGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BglII 495 AGATCT A^GATCT 1301 AGATCT BmyI 430 GAGCAC G[AGT]GC[ACT]^C 760 GGGCTC BpmI 1232 CTGGAG CTGGAG(16/14) 1483 CTGGAG BseRI 336 GAGGAG GAGGAG(10/8) 838 GAGGAG Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C 760 GGGCTC BspMI 1461 ACCTGC ACCTGC(4/8) 48 GCAGGT BsrDI 1343 CATTGC GCAATG(2/0) 2218 GCAATG DraII 263 AGGTCCT [GA]G^G[ACGT]CC[CT] 372 GGGGCCT DraIII 433 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 1258 CACATGGTG EarI 331 GAAGAG CTCTTC(1/4) 697 GAAGAG EcoO109I 263 AGGTCCT [GA]G^G[ACGT]CC[CT] 372 GGGGCCT HincII 1416 GTCAAC GT[CT]^[GA]AC 1475 GTCAAC HindII 1416 GTCAAC GT[CT]^[GA]AC 1475 GTCAAC Ksp632I 331 GAAGAG CTCTTC(1/4) 697 GAAGAG MslI 876 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1827 CACCTGGGTG NdeI 860 CATATG CA^TATG 1546 CATATG PstI 350 CTGCAG CTGCA^G 592 CTGCAG SspI 342 AATATT AAT^ATT 1668 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaII BbvI BfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MunI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI