Restriction map for
pFN21AB6233
(Linear) (Six-base) MAPSORT of: KIBB6233.seq Check: 5058 from: 1 to: 2244
KIBB6233.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 39 1470 2244
Size: 39 1431 774
Fragments arranged by size:
1431 774 39
AclI AA'CG_TT
Cuts at: 0 1288 2244
Size: 1288 956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 386 757 1289 1993 2244
Size: 253 133 371 532 704 251
Fragments arranged by size:
704 532 371 253 251 133
AflIII A'CryG_T
Cuts at: 0 1412 2244
Size: 1412 832
AleI CACnn'nnGTG
Cuts at: 0 1831 2244
Size: 1831 413
ApoI r'AATT_y
Cuts at: 0 86 277 508 1192 2244
Size: 86 191 231 684 1052
Fragments arranged by size:
1052 684 231 191 86
BanI G'GyrC_C
Cuts at: 0 1777 2244
Size: 1777 467
BanII G_rGCy'C
Cuts at: 0 764 2244
Size: 764 1480
BfrBI ATG'CAT
Cuts at: 0 297 2244
Size: 297 1947
BglI GCCn_nnn'nGGC
Cuts at: 0 1313 2244
Size: 1313 931
BglII A'GATC_T
Cuts at: 0 495 1301 2244
Size: 495 806 943
Fragments arranged by size:
943 806 495
BmrI ACTGGGnnnn_n'
Cuts at: 0 135 2244
Size: 135 2109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1356 2244
Size: 1356 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1504 2244
Size: 1253 251 740
Fragments arranged by size:
1253 740 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 1327 2244
Size: 1327 917
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 351 853 2244
Size: 351 502 1391
Fragments arranged by size:
1391 502 351
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 2244
Size: 481 1763
BsiHKAI G_wGCw'C
Cuts at: 0 434 2244
Size: 434 1810
BsiWI C'GTAC_G
Cuts at: 0 1511 2244
Size: 1511 733
Bsp1286I G_dGCh'C
Cuts at: 0 434 764 2244
Size: 434 330 1480
Fragments arranged by size:
1480 434 330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 1470 2244
Size: 39 1431 774
Fragments arranged by size:
1431 774 39
BsrDI GCAATG_nn'
Cuts at: 0 1342 2225 2244
Size: 1342 883 19
Fragments arranged by size:
1342 883 19
BsrFI r'CCGG_y
Cuts at: 0 1892 2244
Size: 1892 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 600 2244
Size: 600 1644
BstEII G'GTnAC_C
Cuts at: 0 1591 2244
Size: 1591 653
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1329 2244
Size: 1329 915
BstYI r'GATC_y
Cuts at: 0 495 1301 1674 2244
Size: 495 806 373 570
Fragments arranged by size:
806 570 495 373
BtgI C'CryG_G
Cuts at: 0 80 152 566 2244
Size: 80 72 414 1678
Fragments arranged by size:
1678 414 80 72
BtsI GCAGTG_nn'
Cuts at: 0 589 2244
Size: 589 1655
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2190 2225 2244
Size: 2190 35 19
Fragments arranged by size:
2190 35 19
DraIII CAC_nnn'GTG
Cuts at: 0 438 1263 2244
Size: 438 825 981
Fragments arranged by size:
981 825 438
EarI CTCTTCn'nnn_
Cuts at: 0 326 692 2244
Size: 326 366 1552
Fragments arranged by size:
1552 366 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 386 757 1253 1289 1504 1993 2244
Size: 253 133 371 496 36 215 489 251
Fragments arranged by size:
496 489 371 253 251 215 133 36
EcoO109I rG'GnC_Cy
Cuts at: 0 264 373 2244
Size: 264 109 1871
Fragments arranged by size:
1871 264 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 228 260 528 560 829 861
Size: 102 32 94 32 268 32 269 32
Cuts at: 861 2244
Size: 1383
Fragments arranged by size:
1383 269 268 102 94 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1418 1477 2244
Size: 1418 59 767
Fragments arranged by size:
1418 767 59
HindIII A'AGCT_T
Cuts at: 0 1561 2244
Size: 1561 683
MfeI C'AATT_G
Cuts at: 0 823 1085 2207 2244
Size: 823 262 1122 37
Fragments arranged by size:
1122 823 262 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 834 2244
Size: 45 789 1410
Fragments arranged by size:
1410 789 45
MslI CAynn'nnrTG
Cuts at: 0 880 1831 2244
Size: 880 951 413
Fragments arranged by size:
951 880 413
NcoI C'CATG_G
Cuts at: 0 80 152 566 2244
Size: 80 72 414 1678
Fragments arranged by size:
1678 414 80 72
NdeI CA'TA_TG
Cuts at: 0 861 1547 2244
Size: 861 686 697
Fragments arranged by size:
861 697 686
NsiI A_TGCA'T
Cuts at: 0 299 2244
Size: 299 1945
NspI r_CATG'y
Cuts at: 0 301 363 900 2244
Size: 301 62 537 1344
Fragments arranged by size:
1344 537 301 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1403 1437 1551 2244
Size: 1403 34 114 693
Fragments arranged by size:
1403 693 114 34
PpuMI rG'GwC_Cy
Cuts at: 0 264 2244
Size: 264 1980
PstI C_TGCA'G
Cuts at: 0 354 596 2244
Size: 354 242 1648
Fragments arranged by size:
1648 354 242
SapI GCTCTTCn'nnn_
Cuts at: 0 692 2244
Size: 692 1552
SexAI A'CCwGG_T
Cuts at: 0 1629 2244
Size: 1629 615
SfcI C'TryA_G
Cuts at: 0 350 516 592 1581 1871 2022 2244
Size: 350 166 76 989 290 151 222
Fragments arranged by size:
989 350 290 222 166 151 76
SspI AAT'ATT
Cuts at: 0 344 1670 2244
Size: 344 1326 574
Fragments arranged by size:
1326 574 344
StyI C'CwwG_G
Cuts at: 0 80 152 566 2244
Size: 80 72 414 1678
Fragments arranged by size:
1678 414 80 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1821 2244
Size: 1821 423
TatI w'GTAC_w
Cuts at: 0 1125 2244
Size: 1125 1119
XbaI T'CTAG_A
Cuts at: 0 378 2244
Size: 378 1866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1846 2244
Size: 1846 398
XmnI GAAnn'nnTTC
Cuts at: 0 1274 1955 2118 2244
Size: 1274 681 163 126
Fragments arranged by size:
1274 681 163 126
Enzymes that do cut:
AarI AclI AcuI AflIII AleI ApoI BanI BanII
BfrBI BglI BglII BmrI BplI BpmI BsaI BseRI
BsgI BsiHKAI BsiWI Bsp1286I BspMI BsrDI BsrFI BstAPI
BstEII BstXI BstYI BtgI BtsI CspCI DraIII EarI
Eco57MI EcoO109I Hin4I HincII HindIII MfeI MmeI MslI
NcoI NdeI NsiI NspI PflMI PpuMI PstI SapI
SexAI SfcI SspI StyI TaqII TatI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI ClaI DraI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI KpnI MluI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XhoI XmaI ZraI