(Circular) (Six-base) MAPSORT of: pFN21AE5519.seq Check: 7128 from: 1 to: 6100 pFN21AE5519 6100 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3910 278 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2537 3443 1937 Size: 600 906 4594 Fragments arranged by size: 4594 906 600 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2437 2558 4163 5547 5815 1632 Size: 805 121 1605 1384 268 1917 Fragments arranged by size: 1917 1605 1384 805 268 121 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 3144 4829 3144 Size: 1685 4415 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AlwNI CAG_nnn'CTG Cuts at: 2903 3843 5415 2903 Size: 940 1572 3588 Fragments arranged by size: 3588 1572 940 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 2178 2202 2209 2378 2430 2511 2618 3409 Size: 685 24 7 169 52 81 107 791 Cuts at: 3409 3635 3787 5896 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 791 685 226 169 152 107 81 52 24 7 AseI AT'TA_AT Cuts at: 160 1032 2319 2556 4654 160 Size: 872 1287 237 2098 1606 Fragments arranged by size: 2098 1606 1287 872 237 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 3425 1870 Size: 73 1482 4545 Fragments arranged by size: 4545 1482 73 BamHI G'GATC_C Cuts at: 3158 3430 3797 3158 Size: 272 367 5461 Fragments arranged by size: 5461 367 272 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3421 4876 618 Size: 325 217 627 1634 1455 1842 Fragments arranged by size: 1842 1634 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3419 1391 Size: 2028 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2068 3270 928 Size: 971 169 1202 3758 Fragments arranged by size: 3758 1202 971 169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2895 2929 4291 4325 1903 Size: 34 958 34 1362 34 3678 Fragments arranged by size: 3678 1362 958 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2627 3291 3992 5202 2627 Size: 664 701 1210 3525 Fragments arranged by size: 3525 1210 701 664 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BfrBI ATG'CAT Cuts at: 2847 2847 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 4161 5317 1790 Size: 2371 1156 2573 Fragments arranged by size: 2573 2371 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3831 4789 442 Size: 416 53 83 399 24 2414 958 1753 Fragments arranged by size: 2414 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2722 4760 1239 Size: 1483 2038 2579 Fragments arranged by size: 2579 2038 1483 Bpu10I CC'TnA_GC Cuts at: 2801 2801 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2446 4225 5090 5388 2446 Size: 1779 865 298 3158 Fragments arranged by size: 3158 1779 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3152 4763 882 Size: 617 1653 1611 2219 Fragments arranged by size: 2219 1653 1611 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3907 4289 4924 275 Size: 53 83 186 936 2374 382 635 1451 Fragments arranged by size: 2374 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2041 2071 1178 Size: 30 165 30 638 30 5207 Fragments arranged by size: 5207 638 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6100 BseYI C'CCAG_C Cuts at: 5303 5799 5303 Size: 496 5604 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5527 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4161 4246 5317 4161 Size: 85 1071 4944 Fragments arranged by size: 4944 1071 85 BsmI GAATG_Cn' Cuts at: 2847 3611 3704 2847 Size: 764 93 5243 Fragments arranged by size: 5243 764 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3419 4161 4246 5317 1391 Size: 399 1629 742 85 1071 2174 Fragments arranged by size: 2174 1629 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 2182 2781 3989 5719 2182 Size: 599 1208 1730 2563 Fragments arranged by size: 2563 1730 1208 599 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3455 844 Size: 2611 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2213 3309 4595 4769 65 Size: 1183 965 1096 1286 174 1396 Fragments arranged by size: 1396 1286 1183 1096 965 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4744 4931 1700 Size: 110 2934 187 2869 Fragments arranged by size: 2934 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2903 794 Size: 2109 3991 BstBI TT'CG_AA Cuts at: 2509 4904 2509 Size: 2395 3705 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstYI r'GATC_y Cuts at: 1972 3004 3158 3278 3430 3797 4182 4199 5651 Size: 1032 154 120 152 367 385 17 1452 Cuts at: 5651 5737 5749 6095 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 154 152 120 86 17 12 BstZ17I GTA'TAC Cuts at: 2538 2538 Size: 6100 Bsu36I CC'TnA_GG Cuts at: 2979 2979 Size: 6100 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 513 Size: 552 939 588 4021 Fragments arranged by size: 4021 939 588 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3014 487 Size: 683 56 1788 3573 Fragments arranged by size: 3573 1788 683 56 BtsI GCAGTG_nn' Cuts at: 2904 3616 4410 4430 2904 Size: 712 794 20 4574 Fragments arranged by size: 4574 794 712 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2254 2289 529 Size: 35 1690 35 4340 Fragments arranged by size: 4340 1690 35 35 DraI TTT'AAA Cuts at: 2376 3404 2376 Size: 1028 5072 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065 Size: 54 1425 50 275 18 1717 889 1629 Cuts at: 6065 8 Size: 43 Fragments arranged by size: 1717 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 4030 1353 Size: 680 1997 3423 Fragments arranged by size: 3423 1997 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 2558 2722 4163 4760 5547 5815 Size: 393 805 121 164 1441 597 787 268 Cuts at: 5815 1239 Size: 1524 Fragments arranged by size: 1524 1441 805 787 597 393 268 164 121 EcoO109I rG'GnC_Cy Cuts at: 2009 2009 Size: 6100 EcoRI G'AATT_C Cuts at: 2209 3409 3787 2209 Size: 1200 378 4522 Fragments arranged by size: 4522 1200 378 EcoRV GAT'ATC Cuts at: 2194 2194 Size: 6100 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 5247 1678 Size: 3569 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2476 2508 2695 2727 3130 Size: 32 135 32 904 32 187 32 403 Cuts at: 3130 3162 4741 4773 4815 4847 1373 Size: 32 1579 32 42 32 2626 Fragments arranged by size: 2626 1579 904 403 187 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3690 669 Size: 988 281 1506 246 3079 Fragments arranged by size: 3079 1506 988 281 246 HindIII A'AGCT_T Cuts at: 748 2019 748 Size: 1271 4829 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 2958 3699 2958 Size: 741 5359 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2404 5214 5398 1305 Size: 1099 2810 184 2007 Fragments arranged by size: 2810 2007 1099 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4278 Fragments arranged by size: 4278 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 4058 4417 4576 518 Size: 648 1230 1662 359 159 2042 Fragments arranged by size: 2042 1662 1230 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3516 4193 5341 5586 1606 Size: 1910 677 1148 245 2120 Fragments arranged by size: 2120 1910 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2592 513 Size: 552 939 588 4021 Fragments arranged by size: 4021 939 588 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NsiI A_TGCA'T Cuts at: 2849 2849 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 3458 5003 1132 Size: 97 2229 1545 2229 Fragments arranged by size: 2229 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PfoI T'CCnGG_A Cuts at: 3154 3154 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2009 2009 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PstI C_TGCA'G Cuts at: 830 3272 3452 830 Size: 2442 180 3478 Fragments arranged by size: 3478 2442 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SexAI A'CCwGG_T Cuts at: 2357 2357 Size: 6100 SfcI C'TryA_G Cuts at: 826 1045 2928 3268 3448 4583 5264 5455 826 Size: 219 1883 340 180 1135 681 191 1471 Fragments arranged by size: 1883 1471 1135 681 340 219 191 180 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2461 4204 5105 5367 820 Size: 197 926 518 1743 901 262 1553 Fragments arranged by size: 1743 1553 926 901 518 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 3184 3339 4024 5 Size: 47 3132 155 685 2081 Fragments arranged by size: 3132 2081 685 155 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 3539 513 Size: 552 939 588 947 3074 Fragments arranged by size: 3074 947 939 588 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2860 4127 4144 4297 4482 4927 325 Size: 1255 1280 1267 17 153 185 445 1498 Fragments arranged by size: 1498 1280 1267 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2954 4346 6058 Size: 274 80 33 51 1443 977 1392 1712 Cuts at: 6058 96 Size: 138 Fragments arranged by size: 1712 1443 1392 977 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3154 3186 6060 6092 3154 Size: 32 2874 32 3162 Fragments arranged by size: 3162 2874 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3332 3436 3332 Size: 104 5996 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 4229 4229 Size: 6100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3908 276 Size: 53 83 186 936 2374 2468 Fragments arranged by size: 2468 2374 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI