################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII BalI BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AvaI 929 CCCGGG C^[CT]CG[GA]G AvrII 546 CCTAGG C^CTAGG BanII 14 GGGCTC G[GA]GC[CT]^C BbsI 900 GAAGAC GAAGAC(2/6) BbvCI 576 GCTGAGG CCTCAGC(-5/-2) BglII 198 AGATCT A^GATCT BlnI 546 CCTAGG C^CTAGG BpuAI 900 GAAGAC GAAGAC(2/6) BseRI 999 GAGGAG GAGGAG(10/8) BsoBI 929 CCCGGG C^[CT]CG[GA]G BsrBI 104 CCGCTC CCGCTC(-3/-3) BstYI 198 AGATCT [GA]^GATC[CT] DpnII 199 GATC ^GATC EaeI 38 TGGCCG [CT]^GGCC[GA] EcoT14I 546 CCTAGG C^C[AT][AT]GG HapII 930 CCGG C^CGG HincII 602 GTCGAC GT[CT]^[GA]AC HindII 602 GTCGAC GT[CT]^[GA]AC HindIII 1010 AAGCTT A^AGCTT HpaII 930 CCGG C^CGG MboI 199 GATC ^GATC MflI 198 AGATCT [GA]^GATC[CT] MspI 930 CCGG C^CGG NdeII 199 GATC ^GATC NspI 114 GCATGT [GA]CATG^[CT] PleI 1002 GAGTC GAGTC(4/5) PvuII 26 CAGCTG CAG^CTG SalI 602 GTCGAC G^TCGAC SapI 106 GCTCTTC GCTCTTC(1/4) Sau3AI 199 GATC ^GATC SmaI 929 CCCGGG CCC^GGG SmlI 408 CTTGAG C^T[CT][GA]AG SspI 425 AATATT AAT^ATT StyI 546 CCTAGG C^C[AT][AT]GG Tsp45I 1004 GTCAC ^GT[GC]AC Tsp509I 273 AATT ^AATT XbaI 226 TCTAGA T^CTAGA XcmI 56 CCATCTTTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 198 AGATCT [GA]^GATC[CT] XmaCI 929 CCCGGG C^CCGGG XmaI 929 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 602 GTCGAC GT^[AC][GT]AC 741 GTCTAC AspHI 7 GAGCAC G[AT]GC[AT]^C 634 GAGCAC BcnI 929 CCCGG CC^[GC]GG 930 CCGGG BlpI 4 GCTGAGC GC^T[ACGT]AGC 556 GCTCAGC Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC 556 GCTCAGC BsaI 99 GAGACC GGTCTC(1/5) 319 GGTCTC BsiHKAI 7 GAGCAC G[AT]GC[AT]^C 634 GAGCAC BsiYI 750 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1018 CCCATCACAGG BslI 750 CCTTTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1018 CCCATCACAGG BsmFI 284 GGGAC GGGAC(10/14) 1016 GTCCC Bsp1407I 525 TGTACA T^GTACA 855 TGTACA BsrGI 525 TGTACA T^GTACA 855 TGTACA Cac8I 582 GCTGGC GC[ACGT]^[ACGT]GC 652 GCCAGC CelII 4 GCTGAGC GC^T[ACGT]AGC 556 GCTCAGC EarI 107 CTCTTC CTCTTC(1/4) 415 GAAGAG FauI 103 CCCGC CCCGC(4/6) 449 CCCGC Ksp632I 107 CTCTTC CTCTTC(1/4) 415 GAAGAG MaeIII 974 GTTAC ^GT[ACGT]AC 1004 GTCAC NciI 929 CCCGG CC^[GC]GG 930 CCGGG SfaNI 660 GATGC GCATC(5/9) 561 GCATC SspBI 525 TGTACA T^GTACA 855 TGTACA TaqI 537 TCGA T^CGA 603 TCGA TfiI 136 GAATC G^A[AT]TC 877 GATTC TthHB8I 537 TCGA T^CGA 603 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BmyI BpmI BsaJI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboII MnlI MseI MspA1I MvaI MwoI NlaIII RsaI ScrFI Tru9I TseI TspRI