################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HapII HgaI HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 436 GACGTC GACGT^C AciI 209 CCGC CCGC(-3/-1) AcyI 436 GACGTC G[GA]^CG[CT]C AseI 1297 ATTAAT AT^TAAT AsnI 1297 ATTAAT AT^TAAT AspHI 891 GAGCTC G[AT]GC[AT]^C BamHI 1160 GGATCC G^GATCC BanII 891 GAGCTC G[GA]GC[CT]^C BciVI 1067 GGATAC GTATCC(6/5) BlpI 481 GCTGAGC GC^T[ACGT]AGC BmyI 891 GAGCTC G[AGT]GC[ACT]^C BpmI 896 CTGGAG CTGGAG(16/14) Bpu1102I 481 GCTGAGC GC^T[ACGT]AGC BsaAI 577 TACGTA [CT]AC^GT[GA] BsaBI 1512 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 436 GACGTC G[GA]^CG[CT]C BsaI 1042 GGTCTC GGTCTC(1/5) BsiHKAI 891 GAGCTC G[AT]GC[AT]^C BsmI 1278 GAATGC GAATGC(1/-1) Bsp1286I 891 GAGCTC G[AGT]GC[ACT]^C BspHI 150 TCATGA T^CATGA BspMI 849 ACCTGC ACCTGC(4/8) BsrBI 209 CCGCTC CCGCTC(-3/-3) BsrDI 1206 CATTGC GCAATG(2/0) Cac8I 477 GCTGGC GC[ACGT]^[ACGT]GC CelII 481 GCTGAGC GC^T[ACGT]AGC CfoI 1404 GCGC GCG^C DsaI 1155 CCATGG C^C[GA][CT]GG EarI 1287 GAAGAG CTCTTC(1/4) EcoNI 861 CCTGCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1340 GAATTC G^AATTC EcoT14I 1155 CCATGG C^C[AT][AT]GG EcoT22I 1280 ATGCAT ATGCA^T HhaI 1404 GCGC GCG^C Hin1I 436 GACGTC G[GA]^CG[CT]C HinP1I 1404 GCGC G^CGC Ksp632I 1287 GAAGAG CTCTTC(1/4) MamI 1512 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1232 CAGCTG C[AC]G^C[GT]G NcoI 1155 CCATGG C^CATGG NsiI 1280 ATGCAT ATGCA^T PflMI 1464 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1297 ATTAAT AT^TAAT PvuII 1232 CAGCTG CAG^CTG RcaI 150 TCATGA T^CATGA SacI 891 GAGCTC GAGCT^C SexAI 1450 ACCTGGT A^CC[AT]GGT SfcI 465 CTGTAG C^T[GA][CT]AG SnaBI 577 TACGTA TAC^GTA StyI 1155 CCATGG C^C[AT][AT]GG TfiI 1176 GATTC G^A[AT]TC Van91I 1464 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AocI 865 CCTAAGG CC^T[ACGT]AGG 1037 CCTTAGG BclI 606 TGATCA T^GATCA 1350 TGATCA Bsp1407I 506 TGTACA T^GTACA 885 TGTACA BsrGI 506 TGTACA T^GTACA 885 TGTACA BstBI 670 TTCGAA TT^CGAA 773 TTCGAA BstYI 989 AGATCC [GA]^GATC[CT] 1160 GGATCC Bsu36I 865 CCTAAGG CC^T[ACGT]AGG 1037 CCTTAGG Cfr13I 959 GGGCC G^G[ACGT]CC 984 GGCCC Eco81I 865 CCTAAGG CC^T[ACGT]AGG 1037 CCTTAGG FbaI 606 TGATCA T^GATCA 1350 TGATCA MflI 989 AGATCC [GA]^GATC[CT] 1160 GGATCC NspV 670 TTCGAA TT^CGAA 773 TTCGAA PleI 836 GACTC GAGTC(4/5) 424 GAGTC Sau96I 959 GGGCC G^G[ACGT]CC 984 GGCCC SfuI 670 TTCGAA TT^CGAA 773 TTCGAA SspBI 506 TGTACA T^GTACA 885 TGTACA SspI 351 AATATT AAT^ATT 675 AATATT TspRI 841 CACTG CA[GC]TG[ACGT][ACGT]^ 1358 CAGTG XhoII 989 AGATCC [GA]^GATC[CT] 1160 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BpuAI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I