(Circular) (Six-base) MAPSORT of: pFN21AE4506.seq Check: 9471 from: 1 to: 6292 pFN21AE4506 6292 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2445 4102 278 Size: 53 83 186 936 909 1657 2468 Fragments arranged by size: 2468 1657 936 909 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 1937 Size: 1698 4594 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2163 4355 5739 6007 1632 Size: 375 156 2192 1384 268 1917 Fragments arranged by size: 2192 1917 1384 375 268 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6292 AlwNI CAG_nnn'CTG Cuts at: 4035 5607 4035 Size: 1572 4720 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 2229 2266 2408 2791 3345 3398 3601 3827 Size: 736 37 142 383 554 53 203 226 Cuts at: 3827 3979 6088 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 736 554 383 226 203 152 142 53 37 AseI AT'TA_AT Cuts at: 160 1032 3303 4846 160 Size: 872 2271 1543 1606 Fragments arranged by size: 2271 1606 1543 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3617 1870 Size: 73 1674 4545 Fragments arranged by size: 4545 1674 73 BamHI G'GATC_C Cuts at: 3165 3622 3989 3165 Size: 457 367 5468 Fragments arranged by size: 5468 457 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3613 5068 618 Size: 325 217 627 1826 1455 1842 Fragments arranged by size: 1842 1826 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2900 3611 1391 Size: 1509 711 4072 Fragments arranged by size: 4072 1509 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2734 2889 3379 928 Size: 971 835 155 490 3841 Fragments arranged by size: 3841 971 835 490 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3066 4184 5394 3066 Size: 1118 1210 3964 Fragments arranged by size: 3964 1210 1118 BclI T'GATC_A Cuts at: 1548 2611 3355 1548 Size: 1063 744 4485 Fragments arranged by size: 4485 1063 744 BfrBI ATG'CAT Cuts at: 3287 3287 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 2487 3709 2487 Size: 1222 5070 Bme1580I G_kGCm'C Cuts at: 1790 4353 5509 1790 Size: 2563 1156 2573 Fragments arranged by size: 2573 2563 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2922 4952 1239 Size: 1683 2030 2579 Fragments arranged by size: 2579 2030 1683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4417 5282 5580 4417 Size: 865 298 5129 Fragments arranged by size: 5129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3053 4955 882 Size: 617 1554 1902 2219 Fragments arranged by size: 2219 1902 1554 617 BsaAI yAC'GTr Cuts at: 493 1204 2584 4083 493 Size: 711 1380 1499 2702 Fragments arranged by size: 2702 1499 1380 711 BsaBI GATnn'nnATC Cuts at: 3521 6286 3521 Size: 2765 3527 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2442 4099 4481 5116 Size: 53 83 186 936 909 1657 382 635 Cuts at: 5116 275 Size: 1451 Fragments arranged by size: 1657 1451 936 909 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3225 3255 1178 Size: 30 165 30 1822 30 4215 Fragments arranged by size: 4215 1822 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6292 BseYI C'CCAG_C Cuts at: 5495 5991 5495 Size: 496 5796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5719 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2900 4353 4438 5509 2900 Size: 1453 85 1071 3683 Fragments arranged by size: 3683 1453 1071 85 BsmI GAATG_Cn' Cuts at: 3289 3803 3896 3289 Size: 514 93 5685 Fragments arranged by size: 5685 514 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2900 3611 4353 4438 5509 1391 Size: 399 1110 711 742 85 1071 2174 Fragments arranged by size: 2174 1110 1071 742 711 399 85 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 2155 4181 5911 2155 Size: 2026 1730 2536 Fragments arranged by size: 2536 2026 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2863 3647 844 Size: 2019 784 3489 Fragments arranged by size: 3489 2019 784 BsrBI CCG'CTC Cuts at: 1394 1823 2216 4179 1394 Size: 429 393 1963 3507 Fragments arranged by size: 3507 1963 429 393 BsrDI GCAATG_nn' Cuts at: 65 1248 3210 4787 4961 65 Size: 1183 1962 1577 174 1396 Fragments arranged by size: 1962 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4936 5123 1700 Size: 110 3126 187 2869 Fragments arranged by size: 3126 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2511 2890 96 Size: 2415 379 3498 Fragments arranged by size: 3498 2415 379 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 2676 2779 5096 2676 Size: 103 2317 3872 Fragments arranged by size: 3872 2317 103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstYI r'GATC_y Cuts at: 1972 2994 3165 3622 3989 4374 4391 5843 5929 Size: 1022 171 457 367 385 17 1452 86 Cuts at: 5929 5941 6287 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1022 457 385 367 346 171 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2871 3043 2871 Size: 172 6120 BtgI C'CryG_G Cuts at: 513 1065 3160 513 Size: 552 2095 3645 Fragments arranged by size: 3645 2095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 2845 3808 4602 4622 2845 Size: 963 794 20 4515 Fragments arranged by size: 4515 963 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3739 4628 6257 Size: 54 1425 50 275 18 1909 889 1629 Cuts at: 6257 8 Size: 43 Fragments arranged by size: 1909 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3287 4222 1353 Size: 1934 935 3423 Fragments arranged by size: 3423 1934 935 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2898 2898 Size: 6292 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2163 2922 4355 4952 5739 6007 Size: 393 375 156 759 1433 597 787 268 Cuts at: 6007 1239 Size: 1524 Fragments arranged by size: 1524 1433 787 759 597 393 375 268 156 EcoNI CCTnn'n_nnAGG Cuts at: 2870 2870 Size: 6292 EcoRI G'AATT_C Cuts at: 3345 3601 3979 3345 Size: 256 378 5658 Fragments arranged by size: 5658 378 256 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 5439 1678 Size: 3761 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2427 2459 4933 4965 5007 Size: 32 135 32 855 32 2474 32 42 Cuts at: 5007 5039 1373 Size: 32 2626 Fragments arranged by size: 2626 2474 855 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 2815 5406 5590 1305 Size: 936 574 2591 184 2007 Fragments arranged by size: 2591 2007 936 574 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4470 Fragments arranged by size: 4470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4250 4609 4768 518 Size: 648 843 2241 359 159 2042 Fragments arranged by size: 2241 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3239 3708 4385 5533 5778 1606 Size: 1633 469 677 1148 245 2120 Fragments arranged by size: 2120 1633 1148 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 3160 513 Size: 552 2095 3645 Fragments arranged by size: 3645 2095 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NsiI A_TGCA'T Cuts at: 3289 3289 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3650 5195 1132 Size: 97 2421 1545 2229 Fragments arranged by size: 2421 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 3475 1108 Size: 2367 3925 PfoI T'CCnGG_A Cuts at: 2130 2786 2130 Size: 656 5636 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3119 3862 3119 Size: 743 5549 PstI C_TGCA'G Cuts at: 830 3644 830 Size: 2814 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 3239 3239 Size: 6292 SacI G_AGCT'C Cuts at: 2900 2900 Size: 6292 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SexAI A'CCwGG_T Cuts at: 3455 3455 Size: 6292 SfcI C'TryA_G Cuts at: 826 1045 2470 3640 4775 5456 5647 826 Size: 219 1425 1170 1135 681 191 1471 Fragments arranged by size: 1471 1425 1170 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4396 5297 5559 820 Size: 197 926 2453 901 262 1553 Fragments arranged by size: 2453 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2584 4083 493 Size: 2091 1499 2702 Fragments arranged by size: 2702 2091 1499 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 2358 2682 4216 5 Size: 47 2306 324 1534 2081 Fragments arranged by size: 2306 2081 1534 324 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 3160 3731 513 Size: 552 2095 571 3074 Fragments arranged by size: 3074 2095 571 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4319 4336 4489 4674 5119 325 Size: 1255 2739 17 153 185 445 1498 Fragments arranged by size: 2739 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2167 2338 2422 Size: 274 80 33 51 1443 190 171 84 Cuts at: 2422 2511 2890 3269 4538 6250 96 Size: 89 379 379 1269 1712 138 Fragments arranged by size: 1712 1443 1269 379 379 274 190 171 138 89 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6252 6284 6252 Size: 32 6260 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 4421 4421 Size: 6292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2443 4100 276 Size: 53 83 186 936 909 1657 2468 Fragments arranged by size: 2468 1657 936 909 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI