(Linear) (Six-base) MAPSORT of: KIEE4506.seq Check: 4198 from: 1 to: 1587 KIEE4506.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AatII G_ACGT'C Cuts at: 0 440 1587 Size: 440 1147 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 158 1587 Size: 2 156 1429 Fragments arranged by size: 1429 156 2 ApoI r'AATT_y Cuts at: 0 224 261 403 786 1340 1393 1587 Size: 224 37 142 383 554 53 194 Fragments arranged by size: 554 383 224 194 142 53 37 AseI AT'TA_AT Cuts at: 0 1298 1587 Size: 1298 289 BamHI G'GATC_C Cuts at: 0 1160 1587 Size: 1160 427 BanII G_rGCy'C Cuts at: 0 895 1587 Size: 895 692 BbsI GAAGACnn'nnnn_ Cuts at: 0 729 884 1374 1587 Size: 729 155 490 213 Fragments arranged by size: 729 490 213 155 BciVI GTATCCnnnnn_n' Cuts at: 0 1061 1587 Size: 1061 526 BclI T'GATC_A Cuts at: 0 606 1350 1587 Size: 606 744 237 Fragments arranged by size: 744 606 237 BfrBI ATG'CAT Cuts at: 0 1282 1587 Size: 1282 305 BlpI GC'TnA_GC Cuts at: 0 482 1587 Size: 482 1105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 917 1587 Size: 917 670 BsaI GGTCTCn'nnnn_ Cuts at: 0 1048 1587 Size: 1048 539 BsaAI yAC'GTr Cuts at: 0 579 1587 Size: 579 1008 BsaBI GATnn'nnATC Cuts at: 0 1516 1587 Size: 1516 71 BsaHI Gr'CG_yC Cuts at: 0 437 1587 Size: 437 1150 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1220 1250 1587 Size: 1220 30 337 Fragments arranged by size: 1220 337 30 BsiHKAI G_wGCw'C Cuts at: 0 895 1587 Size: 895 692 BsmI GAATG_Cn' Cuts at: 0 1284 1587 Size: 1284 303 Bsp1286I G_dGCh'C Cuts at: 0 895 1587 Size: 895 692 BspHI T'CATG_A Cuts at: 0 150 1587 Size: 150 1437 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 858 1587 Size: 858 729 BsrBI CCG'CTC Cuts at: 0 211 1587 Size: 211 1376 BsrDI GCAATG_nn' Cuts at: 0 1205 1587 Size: 1205 382 BsrGI T'GTAC_A Cuts at: 0 506 885 1587 Size: 506 379 702 Fragments arranged by size: 702 506 379 BstBI TT'CG_AA Cuts at: 0 671 774 1587 Size: 671 103 813 Fragments arranged by size: 813 671 103 BstYI r'GATC_y Cuts at: 0 989 1160 1587 Size: 989 171 427 Fragments arranged by size: 989 427 171 Bsu36I CC'TnA_GG Cuts at: 0 866 1038 1587 Size: 866 172 549 Fragments arranged by size: 866 549 172 BtgI C'CryG_G Cuts at: 0 1155 1587 Size: 1155 432 BtsI GCAGTG_nn' Cuts at: 0 840 1587 Size: 840 747 EarI CTCTTCn'nnn_ Cuts at: 0 1282 1587 Size: 1282 305 EcoICRI GAG'CTC Cuts at: 0 893 1587 Size: 893 694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 158 917 1587 Size: 2 156 759 670 Fragments arranged by size: 759 670 156 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 865 1587 Size: 865 722 EcoRI G'AATT_C Cuts at: 0 1340 1587 Size: 1340 247 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 422 454 1587 Size: 422 32 1133 Fragments arranged by size: 1133 422 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 236 810 1587 Size: 236 574 777 Fragments arranged by size: 777 574 236 MspA1I CmG'CkG Cuts at: 0 1234 1587 Size: 1234 353 NcoI C'CATG_G Cuts at: 0 1155 1587 Size: 1155 432 NsiI A_TGCA'T Cuts at: 0 1284 1587 Size: 1284 303 PflMI CCAn_nnn'nTGG Cuts at: 0 1470 1587 Size: 1470 117 PfoI T'CCnGG_A Cuts at: 0 125 781 1587 Size: 125 656 806 Fragments arranged by size: 806 656 125 PsiI TTA'TAA Cuts at: 0 1114 1587 Size: 1114 473 PvuII CAG'CTG Cuts at: 0 1234 1587 Size: 1234 353 SacI G_AGCT'C Cuts at: 0 895 1587 Size: 895 692 SexAI A'CCwGG_T Cuts at: 0 1450 1587 Size: 1450 137 SfcI C'TryA_G Cuts at: 0 465 1587 Size: 465 1122 SnaBI TAC'GTA Cuts at: 0 579 1587 Size: 579 1008 SspI AAT'ATT Cuts at: 0 353 677 1587 Size: 353 324 910 Fragments arranged by size: 910 353 324 StyI C'CwwG_G Cuts at: 0 1155 1587 Size: 1155 432 TatI w'GTAC_w Cuts at: 0 162 333 417 506 885 1264 1587 Size: 162 171 84 89 379 379 323 Fragments arranged by size: 379 379 323 171 162 89 84 ZraI GAC'GTC Cuts at: 0 438 1587 Size: 438 1149 Enzymes that do cut: AatII AcuI ApoI AseI BamHI BanII BbsI BciVI BclI BfrBI BlpI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstBI BstYI Bsu36I BtgI BtsI EarI EcoICRI Eco57MI EcoNI EcoRI Hin4I MmeI MspA1I NcoI NsiI PflMI PfoI PsiI PvuII SacI SexAI SfcI SnaBI SspI StyI TatI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI