################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AlwNI 694 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1069 CCTGAGG CC^T[ACGT]AGG AvaI 835 CTCGGG C^[CT]CG[GA]G BamHI 751 GGATCC G^GATCC BbvCI 1551 CCTCAGC CCTCAGC(-5/-2) BcnI 1518 CCCGG CC^[GC]GG BlpI 1092 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1092 GCTGAGC GC^T[ACGT]AGC BseRI 1217 GAGGAG GAGGAG(10/8) BsmFI 530 GTCCC GGGAC(10/14) BsoBI 835 CTCGGG C^[CT]CG[GA]G BspHI 597 TCATGA T^CATGA Bst1107I 429 GTATAC GTA^TAC BstAPI 817 GCAATGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 785 CCAATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 751 GGATCC [GA]^GATC[CT] BstZ17I 429 GTATAC GTA^TAC Bsu36I 1069 CCTGAGG CC^T[ACGT]AGG CelII 1092 GCTGAGC GC^T[ACGT]AGC CfoI 303 GCGC GCG^C DraII 695 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 781 GACACCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 14 CCACGG C^C[GA][CT]GG EaeI 11 CGGCCA [CT]^GGCC[GA] Eco81I 1069 CCTGAGG CC^T[ACGT]AGG EcoO109I 695 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 1141 GAATTC G^AATTC EcoT22I 1241 ATGCAT ATGCA^T HapII 1519 CCGG C^CGG HhaI 303 GCGC GCG^C HinP1I 303 GCGC G^CGC HincII 322 GTTGAC GT[CT]^[GA]AC HindII 322 GTTGAC GT[CT]^[GA]AC HpaII 1519 CCGG C^CGG HphI 438 GGTGA GGTGA(8/7) MflI 751 GGATCC [GA]^GATC[CT] MslI 1485 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1025 CAGCTG C[AC]G^C[GT]G MspI 1519 CCGG C^CGG NciI 1518 CCCGG CC^[GC]GG NheI 813 GCTAGC G^CTAGC NsiI 1241 ATGCAT ATGCA^T NspI 155 GCATGT [GA]CATG^[CT] PflMI 1613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 590 CTGCAG CTGCA^G PvuII 1025 CAGCTG CAG^CTG RcaI 597 TCATGA T^CATGA SfcI 590 CTGCAG C^T[GA][CT]AG Van91I 1613 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 751 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 103 GTCTAC GT^[AC][GT]AC 429 GTATAC Asp700I 879 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 928 GAAGCCCTTC AspHI 144 GAGCAC G[AT]GC[AT]^C 1095 GAGCAC BbsI 830 GAAGAC GAAGAC(2/6) 1534 GTCTTC BglI 697 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1489 GCCATGCTGGC BpuAI 830 GAAGAC GAAGAC(2/6) 1534 GTCTTC BsaAI 187 TACGTG [CT]AC^GT[GA] 1302 TACGTG BsiHKAI 144 GAGCAC G[AT]GC[AT]^C 1095 GAGCAC Bsp1407I 797 TGTACA T^GTACA 1375 TGTACA BsrDI 666 CATTGC GCAATG(2/0) 817 GCAATG BsrGI 797 TGTACA T^GTACA 1375 TGTACA EcoT14I 605 CCTTGG C^C[AT][AT]GG 1337 CCTTGG HgaI 725 GACGC GACGC(5/10) 528 GCGTC NdeI 950 CATATG CA^TATG 1238 CATATG PleI 514 GACTC GAGTC(4/5) 833 GACTC SmlI 34 CTCAAG C^T[CT][GA]AG 141 CTTGAG SspBI 797 TGTACA T^GTACA 1375 TGTACA StyI 605 CCTTGG C^C[AT][AT]GG 1337 CCTTGG Tsp45I 439 GTGAC ^GT[GC]AC 512 GTGAC XcmI 14 CCACGGGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1609 CCATCCAGTCTGTGG XmnI 879 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 928 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanI BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HindIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI