(Circular) (Six-base) MAPSORT of: pFN21AE4515.seq Check: 9907 from: 1 to: 6346 pFN21AE4515 6346 bp With 182 enzymes: SgfI * March 28, 2012 14:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4156 278 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 2435 3689 1937 Size: 172 326 1254 4594 Fragments arranged by size: 4594 1254 326 172 Acc65I G'GTAC_C Cuts at: 3667 3667 Size: 6346 AclI AA'CG_TT Cuts at: 1563 4473 4846 1563 Size: 2910 373 3063 Fragments arranged by size: 3063 2910 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2673 2697 2726 3236 4409 5793 6061 Size: 399 642 24 29 510 1173 1384 268 Cuts at: 6061 1632 Size: 1917 Fragments arranged by size: 1917 1384 1173 642 510 399 268 29 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6149 AflIII A'CryG_T Cuts at: 1203 5245 6222 1203 Size: 4042 977 1327 Fragments arranged by size: 4042 1327 977 AgeI A'CCGG_T Cuts at: 5177 5177 Size: 6346 AhdI GACnn_n'nnGTC Cuts at: 5075 5075 Size: 6346 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6346 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3037 1184 Size: 1853 4493 AlwNI CAG_nnn'CTG Cuts at: 2704 4089 5661 2704 Size: 1385 1572 3389 Fragments arranged by size: 3389 1572 1385 ApaLI G'TGCA_C Cuts at: 4403 5559 4403 Size: 1156 5190 ApoI r'AATT_y Cuts at: 1493 2404 3065 3146 3655 3881 4033 6142 1493 Size: 911 661 81 509 226 152 2109 1697 Fragments arranged by size: 2109 1697 911 661 509 226 152 81 AseI AT'TA_AT Cuts at: 160 1032 4900 160 Size: 872 3868 1606 Fragments arranged by size: 3868 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 AvaI C'yCGr_G Cuts at: 1870 1943 2840 3671 1870 Size: 73 897 831 4545 Fragments arranged by size: 4545 897 831 73 BamHI G'GATC_C Cuts at: 2756 3676 4043 2756 Size: 920 367 5059 Fragments arranged by size: 5059 920 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3354 3627 3667 5122 Size: 325 217 627 335 1232 273 40 1455 Cuts at: 5122 618 Size: 1842 Fragments arranged by size: 1842 1455 1232 627 335 325 273 217 40 BanII G_rGCy'C Cuts at: 1391 3665 1391 Size: 2274 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2842 3532 928 Size: 971 943 690 3742 Fragments arranged by size: 3742 971 943 690 BbvCI CC'TCA_GC Cuts at: 3557 3557 Size: 6346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4537 4571 1903 Size: 34 2600 34 3678 Fragments arranged by size: 3678 2600 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4238 5448 4238 Size: 1210 5136 BclI T'GATC_A Cuts at: 1548 1548 Size: 6346 BfrBI ATG'CAT Cuts at: 3248 3248 Size: 6346 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 3500 4957 Size: 107 122 71 631 605 1036 792 1457 Cuts at: 4957 136 Size: 1525 Fragments arranged by size: 1525 1457 1036 792 631 605 122 107 71 BglII A'GATC_T Cuts at: 6341 6341 Size: 6346 BlpI GC'TnA_GC Cuts at: 3098 3763 3098 Size: 665 5681 Bme1580I G_kGCm'C Cuts at: 1790 3357 4407 5563 1790 Size: 1567 1050 1156 2573 Fragments arranged by size: 2573 1567 1156 1050 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6346 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2755 4077 5035 Size: 416 53 83 399 24 1338 1322 958 Cuts at: 5035 442 Size: 1753 Fragments arranged by size: 1753 1338 1322 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2822 1056 Size: 1766 4580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2584 2733 5006 1239 Size: 812 533 149 2273 2579 Fragments arranged by size: 2579 2273 812 533 149 Bpu10I CC'TnA_GC Cuts at: 3557 3557 Size: 6346 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2024 2167 4471 5336 5634 2024 Size: 143 2304 865 298 2736 Fragments arranged by size: 2736 2304 865 298 143 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5009 882 Size: 617 3510 2219 Fragments arranged by size: 3510 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2194 3309 4137 493 Size: 711 990 1115 828 2702 Fragments arranged by size: 2702 1115 990 828 711 BsaBI GATnn'nnATC Cuts at: 6340 6340 Size: 6346 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4153 4535 5170 275 Size: 53 83 186 936 2620 382 635 1451 Fragments arranged by size: 2620 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4779 5177 5451 5598 6009 4779 Size: 398 274 147 411 5116 Fragments arranged by size: 5116 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6121 Fragments arranged by size: 6121 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3237 1186 Size: 2051 4295 BseYI C'CCAG_C Cuts at: 5549 6045 5549 Size: 496 5850 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5773 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3796 4557 5585 1540 Size: 275 186 1795 761 1028 2301 Fragments arranged by size: 2301 1795 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2153 3104 4407 4492 5563 2153 Size: 951 1303 85 1071 2936 Fragments arranged by size: 2936 1303 1071 951 85 BsmI GAATG_Cn' Cuts at: 2384 3121 3248 3857 3950 2384 Size: 737 127 609 93 4780 Fragments arranged by size: 4780 737 609 127 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 3104 3357 3665 4407 4492 5563 Size: 399 363 951 253 308 742 85 1071 Cuts at: 5563 1391 Size: 2174 Fragments arranged by size: 2174 1071 951 742 399 363 308 253 85 BspEI T'CCGG_A Cuts at: 6009 6009 Size: 6346 BspHI T'CATG_A Cuts at: 2602 4235 5965 2602 Size: 1633 1730 2983 Fragments arranged by size: 2983 1730 1633 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3701 844 Size: 2857 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4233 1394 Size: 429 2410 3507 Fragments arranged by size: 3507 2410 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2670 2829 4841 5015 65 Size: 1183 1422 159 2012 174 1396 Fragments arranged by size: 2012 1422 1396 1183 174 159 BsrFI r'CCGG_y Cuts at: 1700 1810 4990 5177 1700 Size: 110 3180 187 2869 Fragments arranged by size: 3180 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2802 3380 96 Size: 2706 578 3062 Fragments arranged by size: 3062 2706 578 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6346 BssSI C'ACGA_G Cuts at: 4406 5418 4406 Size: 1012 5334 BstAPI GCAn_nnn'nTGC Cuts at: 794 2828 794 Size: 2034 4312 BstBI TT'CG_AA Cuts at: 5150 5150 Size: 6346 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6346 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2797 Size: 6346 BstYI r'GATC_y Cuts at: 1972 2756 3676 4043 4428 4445 5897 5983 5995 Size: 784 920 367 385 17 1452 86 12 Cuts at: 5995 6341 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 920 784 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2436 2436 Size: 6346 Bsu36I CC'TnA_GG Cuts at: 3075 3075 Size: 6346 BtgI C'CryG_G Cuts at: 513 1065 2004 2019 513 Size: 552 939 15 4840 Fragments arranged by size: 4840 939 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5607 Fragments arranged by size: 5607 683 56 BtsI GCAGTG_nn' Cuts at: 2865 3862 4656 4676 2865 Size: 997 794 20 4535 Fragments arranged by size: 4535 997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6311 DraI TTT'AAA Cuts at: 3650 3650 Size: 6346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2792 5353 809 Size: 507 1476 2561 1802 Fragments arranged by size: 2561 1802 1476 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 3793 4682 Size: 54 1425 50 275 18 186 1777 889 Cuts at: 4682 6311 8 Size: 1629 43 Fragments arranged by size: 1777 1629 1425 889 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3793 1537 Size: 275 1981 4090 Fragments arranged by size: 4090 1981 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2344 2649 3212 3266 4276 1353 Size: 991 305 563 54 1010 3423 Fragments arranged by size: 3423 1010 991 563 305 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4933 5307 5453 387 Size: 1064 138 3344 374 146 1280 Fragments arranged by size: 3344 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2051 2584 2673 2697 2726 2733 Size: 393 399 20 533 89 24 29 7 Cuts at: 2733 3236 4409 5006 5793 6061 1239 Size: 503 1173 597 787 268 1524 Fragments arranged by size: 1524 1173 787 597 533 503 399 393 268 89 29 24 20 7 EcoO109I rG'GnC_Cy Cuts at: 2701 2701 Size: 6346 EcoRI G'AATT_C Cuts at: 3146 3655 4033 3146 Size: 509 378 5459 Fragments arranged by size: 5459 509 378 FspI TGC'GCA Cuts at: 4852 4852 Size: 6346 HaeII r_GCGC'y Cuts at: 1678 5493 1678 Size: 3815 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 3053 3068 3085 Size: 32 135 32 749 32 700 15 17 Cuts at: 3085 3100 3322 3354 4987 5019 5061 5093 1373 Size: 15 222 32 1633 32 42 32 2626 Fragments arranged by size: 2626 1633 749 700 222 135 42 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2329 3690 3936 669 Size: 988 281 391 1361 246 3079 Fragments arranged by size: 3079 1361 988 391 281 246 HindIII A'AGCT_T Cuts at: 748 2998 3187 3549 748 Size: 2250 189 362 3545 Fragments arranged by size: 3545 2250 362 189 HpaI GTT'AAC Cuts at: 3936 3936 Size: 6346 KpnI G_GTAC'C Cuts at: 3671 3671 Size: 6346 MfeI C'AATT_G Cuts at: 3945 3945 Size: 6346 MluI A'CGCG_T Cuts at: 6222 6222 Size: 6346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 5460 5644 1305 Size: 1062 3093 184 2007 Fragments arranged by size: 3093 2007 1062 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4524 Fragments arranged by size: 4524 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3494 4304 4663 4822 518 Size: 648 2328 810 359 159 2042 Fragments arranged by size: 2328 2042 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3032 3762 4439 5587 5832 1606 Size: 1426 730 677 1148 245 2120 Fragments arranged by size: 2120 1426 1148 730 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4855 Fragments arranged by size: 4855 939 552 NdeI CA'TA_TG Cuts at: 387 2956 3244 387 Size: 2569 288 3489 Fragments arranged by size: 3489 2569 288 NheI G'CTAG_C Cuts at: 1052 2818 1052 Size: 1766 4580 NotI GC'GGCC_GC Cuts at: 3793 3793 Size: 6346 NsiI A_TGCA'T Cuts at: 3250 3250 Size: 6346 NspI r_CATG'y Cuts at: 1132 1229 2164 3704 5249 1132 Size: 97 935 1540 1545 2229 Fragments arranged by size: 2229 1545 1540 935 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6346 PciI A'CATG_T Cuts at: 5245 5245 Size: 6346 PflMI CCAn_nnn'nTGG Cuts at: 1108 3624 1108 Size: 2516 3830 PfoI T'CCnGG_A Cuts at: 3522 3522 Size: 6346 PmeI GTTT'AAAC Cuts at: 3650 3650 Size: 6346 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4415 4447 5928 5960 4415 Size: 32 1481 32 4801 Fragments arranged by size: 4801 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6346 PsiI TTA'TAA Cuts at: 3916 3916 Size: 6346 PstI C_TGCA'G Cuts at: 830 2599 3698 830 Size: 1769 1099 3478 Fragments arranged by size: 3478 1769 1099 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6346 PvuII CAG'CTG Cuts at: 3032 3032 Size: 6346 SalI G'TCGA_C Cuts at: 1936 3688 1936 Size: 1752 4594 SbfI CC_TGCA'GG Cuts at: 3698 3698 Size: 6346 ScaI AGT'ACT Cuts at: 4594 6306 4594 Size: 1712 4634 SfcI C'TryA_G Cuts at: 826 1045 2595 3694 4829 5510 5701 826 Size: 219 1550 1099 1135 681 191 1471 Fragments arranged by size: 1550 1471 1135 1099 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6346 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6346 SmaI CCC'GGG Cuts at: 1872 3673 1872 Size: 1801 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2039 2146 4450 5351 5613 820 Size: 197 926 96 107 2304 901 262 1553 Fragments arranged by size: 2304 1553 926 901 262 197 107 96 SnaBI TAC'GTA Cuts at: 493 4137 493 Size: 3644 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6346 SphI G_CATG'C Cuts at: 1132 3704 1132 Size: 2572 3774 SspI AAT'ATT Cuts at: 5 52 4270 5 Size: 47 4218 2081 Fragments arranged by size: 4218 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6346 StyI C'CwwG_G Cuts at: 513 1065 2004 2610 3342 3785 513 Size: 552 939 606 732 443 3074 Fragments arranged by size: 3074 939 732 606 552 443 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2431 3411 4373 4390 4543 4728 5173 Size: 1255 851 980 962 17 153 185 445 Cuts at: 5173 325 Size: 1498 Fragments arranged by size: 1498 1255 980 962 851 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2802 3380 4592 Size: 274 80 33 51 1443 825 578 1212 Cuts at: 4592 6304 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1212 825 578 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3504 3536 6306 6338 3504 Size: 32 2770 32 3512 Fragments arranged by size: 3512 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6346 XbaI T'CTAG_A Cuts at: 3682 3682 Size: 6346 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 3621 2026 Size: 1595 4751 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6346 XmaI C'CCGG_G Cuts at: 1870 3671 1870 Size: 1801 4545 XmnI GAAnn'nnTTC Cuts at: 2888 2937 4475 2888 Size: 49 1538 4759 Fragments arranged by size: 4759 1538 49 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4154 276 Size: 53 83 186 936 2620 2468 Fragments arranged by size: 2620 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI