################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MaeII MamI MluI MroI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Alw44I 1497 GTGCAC G^TGCAC Aor51HI 1701 AGCGCT AGC^GCT ApaLI 1497 GTGCAC G^TGCAC Asp700I 1968 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 1158 TGGCCA TGG^CCA BanII 490 GAGCTC G[GA]GC[CT]^C BbsI 706 GAAGAC GAAGAC(2/6) BglI 409 GCCTTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 706 GAAGAC GAAGAC(2/6) BsaWI 1399 ACCGGA [AT]^CCGG[AT] BsgI 1275 CTGCAC GTGCAG(16/14) BsiEI 1397 CGACCG CG[GA][CT]^CG BsmFI 1035 GTCCC GGGAC(10/14) BspMI 1661 ACCTGC ACCTGC(4/8) BsrDI 1188 CATTGC GCAATG(2/0) BstAPI 510 GCATCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1129 TTCGAA TT^CGAA DraI 783 TTTAAA TTT^AAA DraII 323 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco47III 1701 AGCGCT AGC^GCT EcoNI 1542 CCTCATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 323 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 1584 GATATC GAT^ATC FauI 345 GCGGG CCCGC(4/6) HgaI 88 GCGTC GACGC(5/10) HincII 733 GTTGAC GT[CT]^[GA]AC HindII 733 GTTGAC GT[CT]^[GA]AC MfeI 581 CAATTG C^AATTG MluNI 1158 TGGCCA TGG^CCA MscI 1158 TGGCCA TGG^CCA MunI 581 CAATTG C^AATTG NspI 588 GCATGT [GA]CATG^[CT] NspV 1129 TTCGAA TT^CGAA PflMI 1161 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 323 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 1398 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 305 CAGCTG CAG^CTG SacI 490 GAGCTC GAGCT^C SapI 1756 GAAGAGC GCTCTTC(1/4) SbfI 522 CCTGCAGG CCTGCA^GG SexAI 153 ACCAGGT A^CC[AT]GGT SfuI 1129 TTCGAA TT^CGAA Sse8387I 522 CCTGCAGG CCTGCA^GG StuI 281 AGGCCT AGG^CCT Van91I 1161 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1752 TCTAGA T^CTAGA XcmI 1580 CCAAGATATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1968 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 292 GTAC GT^AC 1171 GTAC AlwNI 79 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 526 CAGGAGCTG AvaII 324 GGTCC G^G[AT]CC 1034 GGTCC BamHI 1517 GGATCC G^GATCC 1538 GGATCC BanI 48 GGCGCC G^G[CT][GA]CC 1456 GGCGCC BbeI 48 GGCGCC GGCGC^C 1456 GGCGCC BfaI 161 CTAG C^TAG 1753 CTAG BpmI 31 CTGGAG CTGGAG(16/14) 94 CTGGAG BsaI 1002 GGTCTC GGTCTC(1/5) 1024 GGTCTC BsmBI 89 CGTCTC CGTCTC(1/5) 1393 GAGACG Cfr13I 324 GGTCC G^G[ACGT]CC 1034 GGTCC EaeI 41 CGGCCA [CT]^GGCC[GA] 1158 TGGCCA EarI 634 GAAGAG CTCTTC(1/4) 1756 GAAGAG EcoRI 298 GAATTC G^AATTC 945 GAATTC HapII 52 CCGG C^CGG 1400 CCGG HpaII 52 CCGG C^CGG 1400 CCGG KasI 48 GGCGCC G^GCGCC 1456 GGCGCC Ksp632I 634 GAAGAG CTCTTC(1/4) 1756 GAAGAG MaeI 161 CTAG C^TAG 1753 CTAG MspI 52 CCGG C^CGG 1400 CCGG NarI 48 GGCGCC GG^CGCC 1456 GGCGCC PstI 523 CTGCAG CTGCA^G 1384 CTGCAG RsaI 292 GTAC GT^AC 1171 GTAC Sau96I 324 GGTCC G^G[ACGT]CC 1034 GGTCC SfaNI 1324 GATGC GCATC(5/9) 510 GCATC SfoI 48 GGCGCC GGC^GCC 1456 GGCGCC SmlI 328 CTTGAG C^T[CT][GA]AG 455 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AcyI AluI AlwI ApoI AspHI BbvI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI DdeI DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI Hin1I HinP1I HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NcoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII