################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BsaBI BsaHI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT14I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 369 TCCGGA T^CCGGA AhdI 1258 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 913 CCTAAGG CC^T[ACGT]AGG AspEI 1258 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1149 CCCGGG C^[CT]CG[GA]G BalI 1136 TGGCCA TGG^CCA BanI 454 GGCACC G^G[CT][GA]CC BbvCI 1339 CCTCAGC CCTCAGC(-5/-2) BbvI 1284 GCTGC GCAGC(8/12) BfaI 148 CTAG C^TAG BlpI 1154 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1154 GCTTAGC GC^T[ACGT]AGC BsaAI 1320 TACGTA [CT]AC^GT[GA] BsaI 171 GAGACC GGTCTC(1/5) BseAI 369 TCCGGA T^CCGGA BsoBI 1149 CCCGGG C^[CT]CG[GA]G Bsp1407I 464 TGTACA T^GTACA BspEI 369 TCCGGA T^CCGGA BspMI 773 ACCTGC ACCTGC(4/8) BsrDI 1164 CATTGC GCAATG(2/0) BsrGI 464 TGTACA T^GTACA BstXI 128 CCAGGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 913 CCTAAGG CC^T[ACGT]AGG CelII 1154 GCTTAGC GC^T[ACGT]AGC DraII 315 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 1136 TGGCCA [CT]^GGCC[GA] Eam1105I 1258 GACCGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 738 GAAGAG CTCTTC(1/4) Eco81I 913 CCTAAGG CC^T[ACGT]AGG EcoNI 909 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 315 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 211 GATATC GAT^ATC FauI 1281 CCCGC CCCGC(4/6) Fnu4HI 1284 GCTGC GC^[ACGT]GC ItaI 1284 GCTGC GC^[ACGT]GC Ksp632I 738 GAAGAG CTCTTC(1/4) MaeI 148 CTAG C^TAG MluNI 1136 TGGCCA TGG^CCA MroI 369 TCCGGA T^CCGGA MscI 1136 TGGCCA TGG^CCA MslI 414 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1282 CCGCTG C[AC]G^C[GT]G PflMI 246 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 315 AGGACCT [GA]G^G[AT]CC[CT] PshAI 80 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SmaI 1149 CCCGGG CCC^GGG SnaBI 1320 TACGTA TAC^GTA SspBI 464 TGTACA T^GTACA TseI 1284 GCTGC G^C[AT]GC Tsp45I 220 GTCAC ^GT[GC]AC Van91I 246 CCATGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1149 CCCGGG C^CCGGG XmaI 1149 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 673 GTAGAC GT^[AC][GT]AC 1309 GTCTAC AciI 1282 CCGC CCGC(-3/-1) 438 GCGG AcsI 307 AAATTT [GA]^AATT[CT] 547 AAATTT ApoI 307 AAATTT [GA]^AATT[CT] 547 AAATTT AspHI 54 GAGCAC G[AT]GC[AT]^C 868 GTGCTC BamHI 41 GGATCC G^GATCC 1074 GGATCC BbsI 204 GAAGAC GAAGAC(2/6) 121 GTCTTC BcnI 1149 CCCGG CC^[GC]GG 1150 CCGGG BmyI 54 GAGCAC G[AGT]GC[ACT]^C 868 GTGCTC BpuAI 204 GAAGAC GAAGAC(2/6) 121 GTCTTC BsaJI 1139 CCAGGG C^C[ACGT][ACGT]GG 1149 CCCGGG BsaWI 332 TCCGGT [AT]^CCGG[AT] 369 TCCGGA BsiHKAI 54 GAGCAC G[AT]GC[AT]^C 868 GTGCTC BsmAI 171 GAGAC GTCTC(1/5) 808 GTCTC Bsp1286I 54 GAGCAC G[AGT]GC[ACT]^C 868 GTGCTC BstYI 41 GGATCC [GA]^GATC[CT] 1074 GGATCC DraI 310 TTTAAA TTT^AAA 1326 TTTAAA MaeII 639 ACGT A^CGT 1321 ACGT MflI 41 GGATCC [GA]^GATC[CT] 1074 GGATCC NciI 1149 CCCGG CC^[GC]GG 1150 CCGGG TfiI 795 GATTC G^A[AT]TC 1295 GAATC XhoII 41 GGATCC [GA]^GATC[CT] 1074 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AfaI AluI AlwI AvaII BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT22I FokI HaeIII HapII HinfI HpaII HphI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV NsiI PleI RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I Tsp509I TspRI