(Circular) (Six-base) MAPSORT of: pFN21AE3672.seq Check: 9827 from: 1 to: 6352 pFN21AE3672 6352 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3695 1937 Size: 1758 4594 Acc65I G'GTAC_C Cuts at: 3673 3673 Size: 6352 AclI AA'CG_TT Cuts at: 1563 2694 4479 4852 1563 Size: 1131 1785 373 3063 Fragments arranged by size: 3063 1785 1131 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2164 2552 2864 3468 4415 5799 6067 1632 Size: 532 388 312 604 947 1384 268 1917 Fragments arranged by size: 1917 1384 947 604 532 388 312 268 AflII C'TTAA_G Cuts at: 820 1017 3287 820 Size: 197 2270 3885 Fragments arranged by size: 3885 2270 197 AflIII A'CryG_T Cuts at: 1203 5251 6228 1203 Size: 4048 977 1327 Fragments arranged by size: 4048 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6352 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 2297 Size: 32 6320 AlwNI CAG_nnn'CTG Cuts at: 2039 4095 5667 2039 Size: 2056 1572 2724 Fragments arranged by size: 2724 2056 1572 ApaLI G'TGCA_C Cuts at: 4409 5565 4409 Size: 1156 5196 ApoI r'AATT_y Cuts at: 1493 2525 2596 2630 2940 3298 3318 3661 3887 Size: 1032 71 34 310 358 20 343 226 Cuts at: 3887 4039 6148 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1032 358 343 310 226 152 71 34 20 AseI AT'TA_AT Cuts at: 160 1032 2667 4906 160 Size: 872 1635 2239 1606 Fragments arranged by size: 2239 1635 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 BamHI G'GATC_C Cuts at: 2495 3488 3682 4049 2495 Size: 993 194 367 4798 Fragments arranged by size: 4798 993 367 194 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3673 5128 618 Size: 325 217 627 1886 1455 1842 Fragments arranged by size: 1886 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3671 1391 Size: 2280 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 5279 Fragments arranged by size: 5279 971 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3346 3380 4543 4577 1903 Size: 34 1409 34 1163 34 3678 Fragments arranged by size: 3678 1409 1163 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2231 4244 5454 2231 Size: 2013 1210 3129 Fragments arranged by size: 3129 2013 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3377 4963 136 Size: 107 122 71 631 605 1705 1586 1525 Fragments arranged by size: 1705 1586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6347 6347 Size: 6352 BlpI GC'TnA_GC Cuts at: 3006 3769 3006 Size: 763 5589 Bme1580I G_kGCm'C Cuts at: 1790 4413 5569 1790 Size: 2623 1156 2573 Fragments arranged by size: 2623 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3024 4083 5041 Size: 416 53 83 399 24 1607 1059 958 Cuts at: 5041 442 Size: 1753 Fragments arranged by size: 1753 1607 1059 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2168 1056 Size: 1112 5240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2505 3096 5012 1239 Size: 1266 591 1916 2579 Fragments arranged by size: 2579 1916 1266 591 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4477 5342 5640 4477 Size: 865 298 5189 Fragments arranged by size: 5189 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2975 5015 882 Size: 617 1476 2040 2219 Fragments arranged by size: 2219 2040 1476 617 BsaAI yAC'GTr Cuts at: 493 1204 4143 493 Size: 711 2939 2702 Fragments arranged by size: 2939 2702 711 BsaBI GATnn'nnATC Cuts at: 6346 6346 Size: 6352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4159 4541 5176 275 Size: 53 83 186 936 2626 382 635 1451 Fragments arranged by size: 2626 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 5183 5457 5604 6015 4785 Size: 398 274 147 411 5122 Fragments arranged by size: 5122 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2368 2398 3383 3413 1178 Size: 30 165 30 965 30 985 30 4117 Fragments arranged by size: 4117 985 965 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2990 2993 1186 Size: 1804 3 4545 Fragments arranged by size: 4545 1804 3 BseYI C'CCAG_C Cuts at: 2227 2321 2344 5555 6051 2227 Size: 94 23 3211 496 2528 Fragments arranged by size: 3211 2528 496 94 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5779 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4563 5591 1540 Size: 275 186 1801 761 1028 2301 Fragments arranged by size: 2301 1801 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3591 4413 4498 5569 3591 Size: 822 85 1071 4374 Fragments arranged by size: 4374 1071 822 85 BsmI GAATG_Cn' Cuts at: 2393 2721 3117 3863 3956 2393 Size: 328 396 746 93 4789 Fragments arranged by size: 4789 746 396 328 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3591 3671 4413 4498 5569 1391 Size: 399 1801 80 742 85 1071 2174 Fragments arranged by size: 2174 1801 1071 742 399 85 80 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 3294 4241 5971 3294 Size: 947 1730 3675 Fragments arranged by size: 3675 1730 947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3707 844 Size: 2863 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4239 1394 Size: 429 2416 3507 Fragments arranged by size: 3507 2416 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4996 5183 1700 Size: 110 3186 187 2869 Fragments arranged by size: 3186 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2120 96 Size: 2024 4328 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 2204 2824 794 Size: 1410 620 4322 Fragments arranged by size: 4322 1410 620 BstBI TT'CG_AA Cuts at: 5156 5156 Size: 6352 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6352 BstYI r'GATC_y Cuts at: 1972 2495 3488 3682 4049 4434 4451 5903 5989 Size: 523 993 194 367 385 17 1452 86 Cuts at: 5989 6001 6347 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 993 523 385 367 346 194 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3045 513 Size: 552 1980 3820 Fragments arranged by size: 3820 1980 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5613 Fragments arranged by size: 5613 683 56 BtsI GCAGTG_nn' Cuts at: 2461 3868 4662 4682 2461 Size: 1407 794 20 4131 Fragments arranged by size: 4131 1407 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6352 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6317 DraI TTT'AAA Cuts at: 2601 3656 2601 Size: 1055 5297 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317 Size: 54 1425 50 275 18 1969 889 1629 Cuts at: 6317 8 Size: 43 Fragments arranged by size: 1969 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2752 4282 1353 Size: 1399 1530 3423 Fragments arranged by size: 3423 1530 1399 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2505 2552 2864 3096 3468 4415 Size: 393 532 341 47 312 232 372 947 Cuts at: 4415 5012 5799 6067 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 947 787 597 532 393 372 341 312 268 232 47 EcoRI G'AATT_C Cuts at: 2940 3298 3661 4039 2940 Size: 358 363 378 5253 Fragments arranged by size: 5253 378 363 358 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 5499 1678 Size: 3821 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2368 2400 3383 3415 4993 Size: 32 135 32 796 32 983 32 1578 Cuts at: 4993 5025 5067 5099 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1578 983 796 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3696 3942 669 Size: 988 281 1758 246 3079 Fragments arranged by size: 3079 1758 988 281 246 HindIII A'AGCT_T Cuts at: 748 2905 3265 3324 748 Size: 2157 360 59 3776 Fragments arranged by size: 3776 2157 360 59 HpaI GTT'AAC Cuts at: 3942 3942 Size: 6352 KpnI G_GTAC'C Cuts at: 3677 3677 Size: 6352 MfeI C'AATT_G Cuts at: 3951 3951 Size: 6352 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2811 5466 5650 1305 Size: 1506 2655 184 2007 Fragments arranged by size: 2655 2007 1506 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4530 Fragments arranged by size: 4530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2714 4310 4669 4828 518 Size: 648 984 564 1596 359 159 2042 Fragments arranged by size: 2042 1596 984 648 564 359 159 MspA1I CmG'CkG Cuts at: 1606 2227 3768 4445 5593 5838 1606 Size: 621 1541 677 1148 245 2120 Fragments arranged by size: 2120 1541 1148 677 621 245 NcoI C'CATG_G Cuts at: 513 1065 3045 513 Size: 552 1980 3820 Fragments arranged by size: 3820 1980 552 NdeI CA'TA_TG Cuts at: 387 2728 387 Size: 2341 4011 NheI G'CTAG_C Cuts at: 1052 2164 1052 Size: 1112 5240 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 2827 3653 3710 5255 1132 Size: 97 1598 826 57 1545 2229 Fragments arranged by size: 2229 1598 1545 826 97 57 PasI CC'CwG_GG Cuts at: 1348 3484 1348 Size: 2136 4216 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6352 PfoI T'CCnGG_A Cuts at: 2949 3215 2949 Size: 266 6086 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PstI C_TGCA'G Cuts at: 830 2456 3704 830 Size: 1626 1248 3478 Fragments arranged by size: 3478 1626 1248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 PvuII CAG'CTG Cuts at: 2227 2227 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SapI GCTCTTCn'nnn_ Cuts at: 2752 2752 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 4600 6312 4600 Size: 1712 4640 SexAI A'CCwGG_T Cuts at: 3507 3507 Size: 6352 SfcI C'TryA_G Cuts at: 826 1045 2452 3700 4835 5516 5707 826 Size: 219 1407 1248 1135 681 191 1471 Fragments arranged by size: 1471 1407 1248 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3287 4456 5357 5619 820 Size: 197 926 1344 1169 901 262 1553 Fragments arranged by size: 1553 1344 1169 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3710 1132 Size: 2578 3774 SspI AAT'ATT Cuts at: 5 52 4276 5 Size: 47 4224 2081 Fragments arranged by size: 4224 2081 47 StuI AGG'CCT Cuts at: 1517 3607 1517 Size: 2090 4262 StyI C'CwwG_G Cuts at: 513 1065 2612 3045 3546 3791 513 Size: 552 1547 433 501 245 3074 Fragments arranged by size: 3074 1547 552 501 433 245 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4379 4396 4549 4734 5179 325 Size: 1255 2799 17 153 185 445 1498 Fragments arranged by size: 2799 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2120 4598 6310 Size: 274 80 33 51 1443 143 2478 1712 Cuts at: 6310 96 Size: 138 Fragments arranged by size: 2478 1712 1443 274 143 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2229 2261 6312 6344 2229 Size: 32 4051 32 2237 Fragments arranged by size: 4051 2237 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3509 1316 Size: 2193 4159 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 4481 4481 Size: 6352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI