Restriction map for
pFN21AE6723
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspI AvaI AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI HindIII HpaI KspI
MfeI MluI MroI MunI NaeI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 949 GGTACC G^GTACC
AccII 370 CGCG CG^CG
AhdI 1630 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1462 CCTGAGG CC^T[ACGT]AGG
ApaI 125 GGGCCC GGGCC^C
Asp718I 949 GGTACC G^GTACC
AspEI 1630 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1569 GTGCTC G[AT]GC[AT]^C
AviII 1985 TGCGCA TGC^GCA
BbeI 1770 GGCGCC GGCGC^C
BfaI 1537 CTAG C^TAG
BlpI 1271 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1271 GCTCAGC GC^T[ACGT]AGC
BsaBI 1725 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1569 GTGCTC G[AT]GC[AT]^C
BsmI 628 GAATGC GAATGC(1/-1)
BspHI 1070 TCATGA T^CATGA
BsrDI 891 CATTGC GCAATG(2/0)
BssSI 1393 CTCGTG CACGAG(-5/-1)
BstAPI 2013 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 370 CGCG CG^CG
Bsu36I 1462 CCTGAGG CC^T[ACGT]AGG
CelII 1271 GCTCAGC GC^T[ACGT]AGC
DraII 589 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1480 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1630 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1462 CCTGAGG CC^T[ACGT]AGG
EcoO109I 589 AGGGCCT [GA]G^G[ACGT]CC[CT]
FspI 1985 TGCGCA TGC^GCA
KasI 1770 GGCGCC G^GCGCC
KpnI 949 GGTACC GGTAC^C
MaeI 1537 CTAG C^TAG
MamI 1725 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 370 CGCG CG^CG
NarI 1770 GGCGCC GG^CGCC
NdeI 881 CATATG CA^TATG
RcaI 1070 TCATGA T^CATGA
SapI 442 GAAGAGC GCTCTTC(1/4)
ScaI 908 AGTACT AGT^ACT
SfoI 1770 GGCGCC GGC^GCC
SphI 1088 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 92 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
1295 CAGACCCTG
BalI 782 TGGCCA TGG^CCA
1223 TGGCCA
BanI 949 GGTACC G^G[CT][GA]CC
1770 GGCGCC
BanII 125 GGGCCC G[GA]GC[CT]^C
445 GAGCCC
BbrPI 1174 CACGTG CAC^GTG
1900 CACGTG
BbsI 436 GAAGAC GAAGAC(2/6)
1663 GTCTTC
BglII 686 AGATCT A^GATCT
1697 AGATCT
BpmI 1584 CTCCAG CTGGAG(16/14)
163 CTGGAG
BpuAI 436 GAAGAC GAAGAC(2/6)
1663 GTCTTC
BsaAI 1174 CACGTG [CT]AC^GT[GA]
1900 CACGTG
BsrBI 961 CCGCTC CCGCTC(-3/-3)
859 GAGCGG
HaeII 992 AGCGCC [GA]GCGC^[CT]
1770 GGCGCC
HgaI 1609 GACGC GACGC(5/10)
1154 GCGTC
HincII 168 GTTGAC GT[CT]^[GA]AC
868 GTCAAC
HindII 168 GTTGAC GT[CT]^[GA]AC
868 GTCAAC
MluNI 782 TGGCCA TGG^CCA
1223 TGGCCA
MscI 782 TGGCCA TGG^CCA
1223 TGGCCA
PflMI 674 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1866 CCAGCCTGTGG
PmaCI 1174 CACGTG CAC^GTG
1900 CACGTG
PmlI 1174 CACGTG CAC^GTG
1900 CACGTG
Van91I 674 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1866 CCAGCCTGTGG
XcmI 1482 CCAAGTGGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1925 CCATCATCATTGTGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AvaII BbvI BcnI BmyI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII