(Circular) (Six-base) MAPSORT of: pFN21AE6723.seq Check: 3015 from: 1 to: 6802 pFN21AE6723 6802 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4612 278 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4145 1937 Size: 2208 4594 Acc65I G'GTAC_C Cuts at: 2954 4123 2954 Size: 1169 5633 AclI AA'CG_TT Cuts at: 1563 4929 5302 1563 Size: 3366 373 3063 Fragments arranged by size: 3366 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2544 2569 3134 3458 4865 6249 6517 Size: 492 420 25 565 324 1407 1384 268 Cuts at: 6517 1632 Size: 1917 Fragments arranged by size: 1917 1407 1384 565 492 420 324 268 25 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6605 AflIII A'CryG_T Cuts at: 1203 3138 3534 3624 5701 6678 1203 Size: 1935 396 90 2077 977 1327 Fragments arranged by size: 2077 1935 1327 977 396 90 AgeI A'CCGG_T Cuts at: 5633 5633 Size: 6802 AhdI GACnn_n'nnGTC Cuts at: 3640 5531 3640 Size: 1891 4911 AleI CACnn'nnGTG Cuts at: 1166 3905 1166 Size: 2739 4063 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3116 1184 Size: 1932 4870 AlwNI CAG_nnn'CTG Cuts at: 2102 3305 4545 6117 2102 Size: 1203 1240 1572 2787 Fragments arranged by size: 2787 1572 1240 1203 ApaI G_GGCC'C Cuts at: 2134 2134 Size: 6802 ApaLI G'TGCA_C Cuts at: 4859 6015 4859 Size: 1156 5646 ApoI r'AATT_y Cuts at: 1493 4111 4337 4489 6598 1493 Size: 2618 226 152 2109 1697 Fragments arranged by size: 2618 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5356 160 Size: 872 4324 1606 Fragments arranged by size: 4324 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 AvaI C'yCGr_G Cuts at: 1870 1943 4127 1870 Size: 73 2184 4545 Fragments arranged by size: 4545 2184 73 BamHI G'GATC_C Cuts at: 4132 4499 4132 Size: 367 6435 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2954 3775 4123 5578 618 Size: 325 217 627 1167 821 348 1455 1842 Fragments arranged by size: 1842 1455 1167 821 627 348 325 217 BanII G_rGCy'C Cuts at: 1391 2134 2454 4121 1391 Size: 743 320 1667 4072 Fragments arranged by size: 4072 1667 743 320 BbeI G_GCGC'C Cuts at: 3779 3779 Size: 6802 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2448 3661 928 Size: 971 549 1213 4069 Fragments arranged by size: 4069 1213 971 549 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4993 5027 1903 Size: 34 3056 34 3678 Fragments arranged by size: 3678 3056 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4694 5904 4694 Size: 1210 5592 BclI T'GATC_A Cuts at: 1548 1548 Size: 6802 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5413 136 Size: 107 122 71 631 605 3741 1525 Fragments arranged by size: 3741 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2691 3702 6797 2691 Size: 1011 3095 2696 Fragments arranged by size: 3095 2696 1011 BlpI GC'TnA_GC Cuts at: 3277 4219 3277 Size: 942 5860 Bme1580I G_kGCm'C Cuts at: 1790 2134 3551 3960 4863 6019 1790 Size: 344 1417 409 903 1156 2573 Fragments arranged by size: 2573 1417 1156 903 409 344 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6802 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2756 4533 5491 Size: 416 53 83 399 24 1339 1777 958 Cuts at: 5491 442 Size: 1753 Fragments arranged by size: 1777 1753 1339 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6802 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2614 2614 Size: 6802 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 3574 5462 1239 Size: 950 1385 1888 2579 Fragments arranged by size: 2579 1888 1385 950 Bpu10I CC'TnA_GC Cuts at: 2544 2544 Size: 6802 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2738 2886 3227 3956 4927 5792 6090 2738 Size: 148 341 729 971 865 298 3450 Fragments arranged by size: 3450 971 865 729 341 298 148 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5465 882 Size: 617 3966 2219 Fragments arranged by size: 3966 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3181 3907 4593 493 Size: 711 1977 726 686 2702 Fragments arranged by size: 2702 1977 726 711 686 BsaBI GATnn'nnATC Cuts at: 3734 6796 3734 Size: 3062 3740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3159 3615 3776 4609 Size: 53 83 186 936 1626 456 161 833 Cuts at: 4609 4991 5626 275 Size: 382 635 1451 Fragments arranged by size: 1626 1451 936 833 635 456 382 186 161 83 53 BsaWI w'CCGG_w Cuts at: 5235 5633 5907 6054 6465 5235 Size: 398 274 147 411 5572 Fragments arranged by size: 5572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2632 2662 1178 Size: 30 165 30 1229 30 5318 Fragments arranged by size: 5318 1229 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2640 3569 1186 Size: 1024 430 929 4419 Fragments arranged by size: 4419 1024 929 430 BseYI C'CCAG_C Cuts at: 2501 3122 3258 6005 6501 2501 Size: 621 136 2747 496 2802 Fragments arranged by size: 2802 2747 621 496 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3467 3929 4046 1163 Size: 573 1731 462 117 3919 Fragments arranged by size: 3919 1731 573 462 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4252 5013 6041 1540 Size: 275 186 2251 761 1028 2301 Fragments arranged by size: 2301 2251 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3578 4863 4948 6019 3578 Size: 1285 85 1071 4361 Fragments arranged by size: 4361 1285 1071 85 BsmI GAATG_Cn' Cuts at: 2639 4313 4406 2639 Size: 1674 93 5035 Fragments arranged by size: 5035 1674 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 2454 3551 3578 3960 4121 4863 Size: 399 344 320 1097 27 382 161 742 Cuts at: 4863 4948 6019 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1097 1071 742 399 382 344 320 161 85 27 BspEI T'CCGG_A Cuts at: 6465 6465 Size: 6802 BspHI T'CATG_A Cuts at: 3075 4691 6421 3075 Size: 1616 1730 3456 Fragments arranged by size: 3456 1730 1616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2381 2615 3304 4157 844 Size: 1537 234 689 853 3489 Fragments arranged by size: 3489 1537 853 689 234 BsrBI CCG'CTC Cuts at: 1394 1823 2866 2968 4689 1394 Size: 429 1043 102 1721 3507 Fragments arranged by size: 3507 1721 1043 429 102 BsrDI GCAATG_nn' Cuts at: 65 1248 2895 5297 5471 65 Size: 1183 1647 2402 174 1396 Fragments arranged by size: 2402 1647 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5446 5633 1700 Size: 110 3636 187 2869 Fragments arranged by size: 3636 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6802 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6802 BssSI C'ACGA_G Cuts at: 3398 4862 5874 3398 Size: 1464 1012 4326 Fragments arranged by size: 4326 1464 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4024 794 Size: 3230 3572 BstBI TT'CG_AA Cuts at: 5606 5606 Size: 6802 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6802 BstYI r'GATC_y Cuts at: 1972 2691 3040 3702 4132 4499 4884 4901 6353 Size: 719 349 662 430 367 385 17 1452 Cuts at: 6353 6439 6451 6797 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 719 662 430 385 367 349 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3468 3468 Size: 6802 BtgI C'CryG_G Cuts at: 513 1065 3456 3756 3952 513 Size: 552 2391 300 196 3363 Fragments arranged by size: 3363 2391 552 300 196 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2391 3960 487 Size: 683 56 1165 1569 3329 Fragments arranged by size: 3329 1569 1165 683 56 BtsI GCAGTG_nn' Cuts at: 3009 3057 3501 4318 5112 5132 3009 Size: 48 444 817 794 20 4679 Fragments arranged by size: 4679 817 794 444 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6802 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6767 DraI TTT'AAA Cuts at: 4106 4106 Size: 6802 DraIII CAC_nnn'GTG Cuts at: 3490 3490 Size: 6802 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5809 809 Size: 507 4493 1802 Fragments arranged by size: 4493 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2787 3228 3882 Size: 54 1425 50 275 18 957 441 654 Cuts at: 3882 4249 5138 6767 8 Size: 367 889 1629 43 Fragments arranged by size: 1629 1425 957 889 654 441 367 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4249 1537 Size: 275 2437 4090 Fragments arranged by size: 4090 2437 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2442 3398 3790 4732 1353 Size: 722 367 956 392 942 3423 Fragments arranged by size: 3423 956 942 722 392 367 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5389 5763 5909 387 Size: 1064 138 3800 374 146 1280 Fragments arranged by size: 3800 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2189 2544 2569 3134 3458 3574 Size: 393 492 65 355 25 565 324 116 Cuts at: 3574 4865 5462 6249 6517 1239 Size: 1291 597 787 268 1524 Fragments arranged by size: 1524 1291 787 597 565 492 393 355 324 268 116 65 25 EcoO109I rG'GnC_Cy Cuts at: 2595 2595 Size: 6802 EcoRI G'AATT_C Cuts at: 4111 4489 4111 Size: 378 6424 FspI TGC'GCA Cuts at: 3992 5308 3992 Size: 1316 5486 HaeII r_GCGC'y Cuts at: 1678 3001 3779 5949 1678 Size: 1323 778 2170 2531 Fragments arranged by size: 2531 2170 1323 778 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2632 2664 2812 2844 3626 Size: 32 135 32 1060 32 148 32 782 Cuts at: 3626 3658 3734 3766 5443 5475 5517 5549 1373 Size: 32 76 32 1677 32 42 32 2626 Fragments arranged by size: 2626 1677 1060 782 148 135 76 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2175 2875 4146 4392 669 Size: 988 281 237 700 1271 246 3079 Fragments arranged by size: 3079 1271 988 700 281 246 237 HindIII A'AGCT_T Cuts at: 748 748 Size: 6802 HpaI GTT'AAC Cuts at: 4392 4392 Size: 6802 KasI G'GCGC_C Cuts at: 3775 3775 Size: 6802 KpnI G_GTAC'C Cuts at: 2958 4127 2958 Size: 1169 5633 MfeI C'AATT_G Cuts at: 4401 4401 Size: 6802 MluI A'CGCG_T Cuts at: 6678 6678 Size: 6802 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3711 5916 6100 1305 Size: 2406 2205 184 2007 Fragments arranged by size: 2406 2205 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2789 3230 10 Size: 54 1425 343 957 441 3582 Fragments arranged by size: 3582 1425 957 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3179 3423 3629 3905 3938 4760 5119 Size: 648 2013 244 206 276 33 822 359 Cuts at: 5119 5278 518 Size: 159 2042 Fragments arranged by size: 2042 2013 822 648 359 276 244 206 159 33 MspA1I CmG'CkG Cuts at: 1606 2167 2501 2510 3156 3481 4040 4218 4895 Size: 561 334 9 646 325 559 178 677 Cuts at: 4895 6043 6288 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 646 561 559 334 325 245 178 9 NarI GG'CG_CC Cuts at: 3776 3776 Size: 6802 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6250 NdeI CA'TA_TG Cuts at: 387 2887 387 Size: 2500 4302 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6802 NotI GC'GGCC_GC Cuts at: 4249 4249 Size: 6802 NspI r_CATG'y Cuts at: 1132 1229 3097 3142 3538 3628 4160 5705 1132 Size: 97 1868 45 396 90 532 1545 2229 Fragments arranged by size: 2229 1868 1545 532 396 97 90 45 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6802 PciI A'CATG_T Cuts at: 3138 3534 3624 5701 3138 Size: 396 90 2077 4239 Fragments arranged by size: 4239 2077 396 90 PflMI CCAn_nnn'nTGG Cuts at: 1108 2685 3877 1108 Size: 1577 1192 4033 Fragments arranged by size: 4033 1577 1192 PfoI T'CCnGG_A Cuts at: 2622 3644 2622 Size: 1022 5780 PmeI GTTT'AAAC Cuts at: 4106 4106 Size: 6802 PmlI CAC'GTG Cuts at: 3181 3907 3181 Size: 726 6076 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3125 3157 4871 4903 6384 6416 3125 Size: 32 1714 32 1481 32 3511 Fragments arranged by size: 3511 1714 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6802 PsiI TTA'TAA Cuts at: 4372 4372 Size: 6802 PspOMI G'GGCC_C Cuts at: 2130 2130 Size: 6802 PstI C_TGCA'G Cuts at: 830 2500 3275 3683 4154 830 Size: 1670 775 408 471 3478 Fragments arranged by size: 3478 1670 775 471 408 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6802 PvuII CAG'CTG Cuts at: 2167 2501 2510 3481 4040 2167 Size: 334 9 971 559 4929 Fragments arranged by size: 4929 971 559 334 9 SalI G'TCGA_C Cuts at: 1936 4144 1936 Size: 2208 4594 SapI GCTCTTCn'nnn_ Cuts at: 2442 2442 Size: 6802 SbfI CC_TGCA'GG Cuts at: 4154 4154 Size: 6802 ScaI AGT'ACT Cuts at: 2915 5050 6762 2915 Size: 2135 1712 2955 Fragments arranged by size: 2955 2135 1712 SexAI A'CCwGG_T Cuts at: 2946 3195 4074 2946 Size: 249 879 5674 Fragments arranged by size: 5674 879 249 SfcI C'TryA_G Cuts at: 826 1045 2496 3106 3271 3679 4150 5285 5966 Size: 219 1451 610 165 408 471 1135 681 Cuts at: 5966 6157 826 Size: 191 1471 Fragments arranged by size: 1471 1451 1135 681 610 471 408 219 191 165 SfoI GGC'GCC Cuts at: 3777 3777 Size: 6802 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6802 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6802 SmaI CCC'GGG Cuts at: 1872 4129 1872 Size: 2257 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 2901 3242 3971 4906 5807 Size: 197 926 774 184 341 729 935 901 Cuts at: 5807 6069 820 Size: 262 1553 Fragments arranged by size: 1553 935 926 901 774 729 341 262 197 184 SnaBI TAC'GTA Cuts at: 493 4593 493 Size: 4100 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6802 SphI G_CATG'C Cuts at: 1132 3097 4160 1132 Size: 1965 1063 3774 Fragments arranged by size: 3774 1965 1063 SspI AAT'ATT Cuts at: 5 52 4726 5 Size: 47 4674 2081 Fragments arranged by size: 4674 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6802 StyI C'CwwG_G Cuts at: 513 1065 2454 2553 3663 4241 513 Size: 552 1389 99 1110 578 3074 Fragments arranged by size: 3074 1389 1110 578 552 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 2333 3749 3917 3997 4829 4846 Size: 1255 612 141 1416 168 80 832 17 Cuts at: 4846 4999 5184 5629 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1416 1255 832 612 445 185 168 153 141 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2913 3033 3135 Size: 274 80 33 51 1443 936 120 102 Cuts at: 3135 5048 6760 96 Size: 1913 1712 138 Fragments arranged by size: 1913 1712 1443 936 274 138 120 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3448 3480 6762 6794 3448 Size: 32 3282 32 3456 Fragments arranged by size: 3456 3282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6802 XbaI T'CTAG_A Cuts at: 4138 4138 Size: 6802 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3494 3937 3494 Size: 443 6359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6802 XmaI C'CCGG_G Cuts at: 1870 4127 1870 Size: 2257 4545 XmnI GAAnn'nnTTC Cuts at: 4931 4931 Size: 6802 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4610 276 Size: 53 83 186 936 3076 2468 Fragments arranged by size: 3076 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI